Introduction to DNA: Structure and Function

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Questions and Answers

Which of the following best describes the primary function of DNA?

  • Catalyzing metabolic reactions within the cell.
  • Storing and transmitting genetic information. (correct)
  • Facilitating the transport of molecules across cell membranes.
  • Providing immediate energy for cellular activities.

What is the relationship between a DNA segment, a gene, and a trait?

  • A gene codes for a DNA segment, which directly expresses a trait.
  • A trait codes for a DNA segment, which in turn forms a gene.
  • A DNA segment codes for a gene, which influences a specific trait. (correct)
  • A trait is composed of multiple genes, each controlling a different DNA segment.

Who are credited with establishing the structure of DNA, with crucial support from Rosalind Franklin's X-ray images?

  • Linus Pauling and Erwin Chargaff
  • Gregor Mendel and Thomas Hunt Morgan
  • James Watson and Francis Crick (correct)
  • Rosalind Franklin and Maurice Wilkins

What is the repeating subunit that forms a DNA molecule?

<p>Nucleotide (A)</p> Signup and view all the answers

Which components are included in a single nucleotide?

<p>A phosphate group, a deoxyribose sugar, and a nitrogenous base. (D)</p> Signup and view all the answers

According to the base-pair rule, which of the following pairings occurs in DNA?

<p>Adenine pairs with Thymine, Guanine pairs with Cytosine. (D)</p> Signup and view all the answers

If one strand of DNA has the sequence 5'-A-T-T-C-G-A-3', what is the sequence of the complementary strand?

<p>3'-T-A-A-G-C-T-5' (B)</p> Signup and view all the answers

Before cell division, DNA undergoes replication. What is the outcome of this process?

<p>Two DNA molecules, each containing one original and one new strand. (D)</p> Signup and view all the answers

How does RNA differ structurally from DNA?

<p>RNA contains ribose sugar, is single-stranded, and contains uracil. (B)</p> Signup and view all the answers

During transcription, what type of molecule is produced?

<p>mRNA (C)</p> Signup and view all the answers

If a DNA sequence is 5'-T-A-C-A-G-T-3', what would be the corresponding mRNA sequence produced during transcription?

<p>3'-A-U-G-U-C-A-5' (D)</p> Signup and view all the answers

What is the primary role of ribosomes in protein synthesis?

<p>Translating mRNA into a protein. (C)</p> Signup and view all the answers

How does mRNA direct protein synthesis?

<p>It dictates the sequence of amino acids for the protein. (C)</p> Signup and view all the answers

In the central dogma of molecular biology, what is the correct flow of genetic information?

<p>DNA → RNA → Protein (C)</p> Signup and view all the answers

What is a codon and what does it specify?

<p>A sequence of three nucleotides on mRNA that specifies an amino acid. (C)</p> Signup and view all the answers

During translation, a particular mRNA codon is 'AUG'. What amino acid does this codon specify, and what role does it play in protein synthesis?

<p>Methionine; signals the start of translation. (B)</p> Signup and view all the answers

Which process describes how mRNA is used to synthesize proteins?

<p>Translation (B)</p> Signup and view all the answers

Where do transcription and translation occur in eukaryotic cells?

<p>Transcription in the nucleus, translation in the cytoplasm. (C)</p> Signup and view all the answers

What is the consequence of a change (mutation) in a single nucleotide within a coding region of DNA?

<p>The shape of the protein will change and the protein will not function properly. (B)</p> Signup and view all the answers

A mutation in a gene results in a change in the amino acid sequence of the encoded protein. If the original codon was 'GGU' (Specifying Glycine), and the mutated codon is 'GGU', what most likely happened?

<p>A silent or synonymous mutation occurred. (A)</p> Signup and view all the answers

What is the process by which DNA makes a copy of itself during cell division?

<p>Replication (B)</p> Signup and view all the answers

In DNA replication, which of the following describes the role of the 'old' or original strands?

<p>They serve as a template for the synthesis of new DNA strands. (D)</p> Signup and view all the answers

Given the DNA template strand 3'-TTCAGTCGT-5', what would be the corresponding mRNA sequence after transcription?

<p>5'-AAGUCAGCA-3' (C)</p> Signup and view all the answers

Sickle cell anemia is caused by a mutation that affects the shape and function of hemoglobin. Which of the following best describes the nature of this mutation?

<p>A change in a single nucleotide leads to a different amino acid in the hemoglobin protein. (A)</p> Signup and view all the answers

Flashcards

What does DNA stand for?

Deoxyribonucleic Acid, found in all cells and packed tightly within chromosomes in the nucleus.

What are genes?

Segments of DNA that code for traits.

What is DNA?

A molecule with a double helix structure established by Watson and Crick.

What are nucleotides?

Repeating subunits that compose DNA.

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What are the 3 parts of a nucleotide?

Phosphate, deoxyribose sugar, and a base (A, T, G, C).

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What is the Base-Pair Rule?

Adenine pairs with Thymine (A-T); Guanine pairs with Cytosine (G-C).

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What is DNA Replication?

The process by which DNA makes a copy of itself during cell division.

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What is the difference between DNA and RNA?

DNA-Double helix, deoxyribose sugar, found in the nucleus. RNA-Single strand, ribose sugar, contains Uracil base.

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What is Transcription?

DNA is made into mRNA.

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What is the function of messenger RNA(mRNA)?

mRNA leaves the nucleus and takes the code to ribosomes.

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What is a codon?

Every 3 bases on mRNA; specifies an amino acid.

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What is Translation?

The message on mRNA is used to make protein.

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What can a change to a single nucleotide do?

A change (mutation) in a single nucleotide can lead to wrong amino acid & wrong protein shape.

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Study Notes

  • DNA is the blueprint of life
  • DNA stands for deoxyribonucleic acid.
  • Genes are segments of DNA that code for traits.

DNA Location & Packaging

  • DNA is found in all cells.
  • It is packed tightly within chromosomes inside the nucleus.

DNA Traits

  • Small changes in DNA can lead to big changes in traits.
  • Some traits, like curly coats in dogs, are easily seen.
  • Other traits, like the ability to taste chemicals such as PTC, cannot be seen.

Discovering DNA Structure

  • James Watson and Francis Crick established the structure of DNA.
  • Rosalind Franklin's X-ray images of DNA were crucial for determining its shape.

DNA Composition

  • DNA has repeating subunits called nucleotides.
  • Nucleotides have three parts: a phosphate group, deoxyribose (sugar), and a base.
  • The bases are adenine (A), thymine (T), guanine (G), and cytosine (C).

Base-Pair Rule

  • Adenine (A) always pairs with thymine (T).
  • Guanine (G) always pairs with cytosine (C).
  • The sides of the DNA ladder consist of phosphate and sugar
  • Hydrogen bonds hold the sides of the DNA ladder together.

DNA Replication

  • DNA replicates via a process where it makes a copy of itself during cell division.
  • The double helix unzips, and new bases (A, T, G, C) are added.
  • Two new strands are created, each containing half of the original strand.

DNA vs. RNA

  • DNA is a double helix found in the nucleus, containing deoxyribose.
  • RNA is a single strand with three forms (mRNA, tRNA, rRNA), containing ribose and uracil.

Transcription

  • DNA is made into mRNA.
  • RNA has uracil instead of thymine.
  • mRNA leaves the nucleus and takes the code to ribosomes

Translation

  • The message on mRNA is used to make protein.
  • The shapes and composition of a protein determine its functionality

Quick Recap

  • DNA is made into mRNA via transcription, which occurs in the nucleus.
  • mRNA carries the code to ribosomes for translation, and amino acids form a protein.

Codons

  • Each set of 3 bases on mRNA makes a codon.
  • A codon corresponds to one amino acid.

How RNA Makes Protein

  • RNA instructs ribosomes on what protein to build.
  • Every 3 letters (AAA) specifies a single amino acid.
  • Proteins are made of many amino acids.

Sickle Cell Disease

  • Sickle cell disease results from a change (mutation) in a single nucleotide.
  • This mutation leads to the wrong amino acid being produced, resulting in a protein with the wrong shape.

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