Introduction to Chromatography: The Science of Separation

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Questions and Answers

What did Mikhail Tswett use to separate plant pigments in the first true chromatography?

  • Alumina
  • Silica gel
  • Activated charcoal
  • Finely divided chalk (calcium carbonate CaCO3) (correct)

What does the term 'chromatography' mean?

  • Component Identification
  • Quantitative Analysis
  • Color Writing (correct)
  • Mixture Separation

Which components can be separated using chromatography technique?

  • Sugars, salts, acids, bases
  • Pigments, dyes, amino acids, vitamins, polymers (correct)
  • Metals, alloys, ceramics, minerals
  • Gases, liquids, solids, plasma

What is the primary purpose of chromatography?

<p>Separating mixtures into their components for analysis and purification (A)</p> Signup and view all the answers

In which year was liquid chromatography first invented and named by Mikhail Tswett?

<p>1903 (B)</p> Signup and view all the answers

What is the mobile phase in gas chromatography?

<p>Gas (D)</p> Signup and view all the answers

In liquid solid chromatography, what is the nature of the stationary phase?

<p>Solid (B)</p> Signup and view all the answers

What is the nature of the stationary phase in liquid liquid chromatography?

<p>Liquid (D)</p> Signup and view all the answers

What type of chromatography uses a planar flat plate for the stationary phase?

<p>Planar chromatography (A)</p> Signup and view all the answers

What type of chromatography involves a chemically and permanently held stationary phase to a porous solid?

<p>Bonded phase chromatography (BPC) (A)</p> Signup and view all the answers

Flashcards

Chromatography

The process of separating mixtures into their individual components.

What did Tswett use in his first chromatography?

Finely divided chalk (calcium carbonate CaCO3) was used as the stationary phase in Tswett's first chromatography experiment.

Mobile phase in Gas Chromatography

The mobile phase in gas chromatography is a gas, typically an inert gas like helium or nitrogen.

What are the phases in chromatography?

The mobile phase is a liquid or gas. The stationary phase is a solid or liquid.

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Stationary phase in Liquid-Solid Chromatography

The stationary phase in liquid-solid chromatography is a solid material, often silica gel or alumina.

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Stationary phase in Liquid-Liquid Chromatography

The stationary phase in liquid-liquid chromatography is a liquid, usually a liquid phase that is immiscible with the mobile phase.

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Planar Chromatography

Planar chromatography involves a flat stationary phase, like a thin layer of silica gel on a plate or a sheet of paper.

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Bonded Phase Chromatography

In bonded phase chromatography, the stationary phase is chemically bonded to the surface of a porous solid, creating a stable, non-removable, and selective surface.

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What is the purpose of chromatography?

Chromatography is used to separate and purify substances based on their different affinities for the mobile and stationary phases.

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What substances can be separated by chromatography?

Examples of substances that can be separated by chromatography include pigments, dyes, amino acids, vitamins, and polymers.

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