Chromatography

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Questions and Answers

What does the term 'chromatography' mean?

  • Density-based separation
  • Molecule separation
  • Mobile phase movement
  • Color writing (correct)

What is the formula for calculating Retardation Factor (Rf) in chromatography?

  • Distance traveled by a component from origin - Distance traveled by mobile phase from origin
  • Distance traveled by mobile phase from origin - Distance traveled by a component from origin
  • Distance traveled by a component from origin / Distance traveled by mobile phase from origin (correct)
  • Distance traveled by mobile phase from origin / Distance traveled by a component from origin

In chromatography, what does a small Retardation Factor (Rf) indicate?

  • High solubility and low adsorption
  • Low solubility and low adsorption
  • Low solubility and high adsorption (correct)
  • High solubility and high adsorption

What is the principle behind centrifugation?

<p>Separation based on density (B)</p> Signup and view all the answers

Which type of chromatography uses a stationary phase of gel?

<p>Gel-permeation chromatography (A)</p> Signup and view all the answers

What is the mobile phase in paper chromatography?

<p>Water (D)</p> Signup and view all the answers

What does a large Retardation Factor (Rf) in chromatography indicate?

<p>High solubility and low adsorption (D)</p> Signup and view all the answers

Chromatography is a separation technique based on the movement of molecules through a stationary phase and a mobile phase.

<p>True (A)</p> Signup and view all the answers

The Retardation Factor (Rf) in chromatography is small when solubility is small and adsorption is great.

<p>True (A)</p> Signup and view all the answers

Centrifugation separates molecules based on their molecular weight.

<p>False (B)</p> Signup and view all the answers

Paper chromatography uses a mobile phase of water and a stationary phase of paper.

<p>True (A)</p> Signup and view all the answers

Gel-permeation chromatography is also known as molecular sieve chromatography.

<p>True (A)</p> Signup and view all the answers

Spectrophotometry is a technique used in chromatography to detect and quantify separated components.

<p>True (A)</p> Signup and view all the answers

What type of chromatography uses a mobile phase of water and a stationary phase of paper?

<p>paper chromatography (D)</p> Signup and view all the answers

What does a large Retardation Factor (Rf) in chromatography indicate?

<p>Great solubility and small adsorption (B)</p> Signup and view all the answers

What is the principle behind centrifugation?

<p>Separation based on density (C)</p> Signup and view all the answers

What does a small Retardation Factor (Rf) in chromatography indicate?

<p>Small solubility and great adsorption (D)</p> Signup and view all the answers

What is the mobile phase in paper chromatography?

<p>Water (A)</p> Signup and view all the answers

A photometer is device that measures?

<p>light intensity (A)</p> Signup and view all the answers

What is the primary characteristic of anemia?

<p>Low Hemoglobin levels (B)</p> Signup and view all the answers

Which component of the blood is primarily affected in anemia?

<p>Red Blood Cells (C)</p> Signup and view all the answers

Which of the following best describes plasma?

<p>The liquid, cell-free part of blood treated with anti-coagulants (A)</p> Signup and view all the answers

What is the primary difference between plasma and serum?

<p>Plasma is anti-coagulated while serum is clotted (A)</p> Signup and view all the answers

What is the key characteristic that distinguishes serum from plasma?

<p>Serum is the liquid part of blood after coagulation, devoid of clotting factors (D)</p> Signup and view all the answers

What causes the suspended particles to collect as a pellet in the tube bottom during centrifugation?

<p>Centrifugal force (D)</p> Signup and view all the answers

What is the primary force responsible for separating molecules based on their molecular weight in a centrifuge?

<p>Centrifugal force (C)</p> Signup and view all the answers

What does the hematocrit value of 45% of total volume of centrifuged blood represent?

<p>The combined volume of red cells and buffy coat (A)</p> Signup and view all the answers

In a centrifuged blood sample, what does the 'buffy coat' mainly consist of?

<p>White blood cells and platelets (A)</p> Signup and view all the answers

What does a photometer primarily measure?

<p>Light intensity (A)</p> Signup and view all the answers

What is the hemoglobin concentration for females ?

<p>14+ 2 g/dL (B)</p> Signup and view all the answers

What property of a sample can be determined using a photometer?

<p>Light absorption in specific wavelength (C)</p> Signup and view all the answers

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