31 Questions
What does the term 'chromatography' mean?
Color writing
What is the formula for calculating Retardation Factor (Rf) in chromatography?
Distance traveled by a component from origin / Distance traveled by mobile phase from origin
In chromatography, what does a small Retardation Factor (Rf) indicate?
Low solubility and high adsorption
What is the principle behind centrifugation?
Separation based on density
Which type of chromatography uses a stationary phase of gel?
Gel-permeation chromatography
What is the mobile phase in paper chromatography?
Water
What does a large Retardation Factor (Rf) in chromatography indicate?
High solubility and low adsorption
Chromatography is a separation technique based on the movement of molecules through a stationary phase and a mobile phase.
True
The Retardation Factor (Rf) in chromatography is small when solubility is small and adsorption is great.
True
Centrifugation separates molecules based on their molecular weight.
False
Paper chromatography uses a mobile phase of water and a stationary phase of paper.
True
Gel-permeation chromatography is also known as molecular sieve chromatography.
True
Spectrophotometry is a technique used in chromatography to detect and quantify separated components.
True
What type of chromatography uses a mobile phase of water and a stationary phase of paper?
paper chromatography
What does a large Retardation Factor (Rf) in chromatography indicate?
Great solubility and small adsorption
What is the principle behind centrifugation?
Separation based on density
What does a small Retardation Factor (Rf) in chromatography indicate?
Small solubility and great adsorption
What is the mobile phase in paper chromatography?
Water
A photometer is device that measures?
light intensity
What is the primary characteristic of anemia?
Low Hemoglobin levels
Which component of the blood is primarily affected in anemia?
Red Blood Cells
Which of the following best describes plasma?
The liquid, cell-free part of blood treated with anti-coagulants
What is the primary difference between plasma and serum?
Plasma is anti-coagulated while serum is clotted
What is the key characteristic that distinguishes serum from plasma?
Serum is the liquid part of blood after coagulation, devoid of clotting factors
What causes the suspended particles to collect as a pellet in the tube bottom during centrifugation?
Centrifugal force
What is the primary force responsible for separating molecules based on their molecular weight in a centrifuge?
Centrifugal force
What does the hematocrit value of 45% of total volume of centrifuged blood represent?
The combined volume of red cells and buffy coat
In a centrifuged blood sample, what does the 'buffy coat' mainly consist of?
White blood cells and platelets
What does a photometer primarily measure?
Light intensity
What is the hemoglobin concentration for females ?
14+ 2 g/dL
What property of a sample can be determined using a photometer?
Light absorption in specific wavelength
Learn about the basics of chromatography, a separation technique for molecules and components. Understand the principles, uses, and applications of chromatography in diagnostic tools and the separation of mixture components.
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