Chromatography
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Questions and Answers

What does the term 'chromatography' mean?

  • Density-based separation
  • Molecule separation
  • Mobile phase movement
  • Color writing (correct)

    • What is the formula for calculating Retardation Factor (Rf) in chromatography?

  • Distance traveled by a component from origin - Distance traveled by mobile phase from origin
  • Distance traveled by mobile phase from origin - Distance traveled by a component from origin
  • Distance traveled by a component from origin / Distance traveled by mobile phase from origin (correct)
  • Distance traveled by mobile phase from origin / Distance traveled by a component from origin

    • In chromatography, what does a small Retardation Factor (Rf) indicate?

  • High solubility and low adsorption
  • Low solubility and low adsorption
  • Low solubility and high adsorption (correct)
  • High solubility and high adsorption

    • What is the principle behind centrifugation?

    <p>Separation based on density</p> Signup and view all the answers

    Which type of chromatography uses a stationary phase of gel?

    <p>Gel-permeation chromatography</p> Signup and view all the answers

    What is the mobile phase in paper chromatography?

    <p>Water</p> Signup and view all the answers

    What does a large Retardation Factor (Rf) in chromatography indicate?

    <p>High solubility and low adsorption</p> Signup and view all the answers

    Chromatography is a separation technique based on the movement of molecules through a stationary phase and a mobile phase.

    <p>True</p> Signup and view all the answers

    The Retardation Factor (Rf) in chromatography is small when solubility is small and adsorption is great.

    <p>True</p> Signup and view all the answers

    Centrifugation separates molecules based on their molecular weight.

    <p>False</p> Signup and view all the answers

    Paper chromatography uses a mobile phase of water and a stationary phase of paper.

    <p>True</p> Signup and view all the answers

    Gel-permeation chromatography is also known as molecular sieve chromatography.

    <p>True</p> Signup and view all the answers

    Spectrophotometry is a technique used in chromatography to detect and quantify separated components.

    <p>True</p> Signup and view all the answers

    What type of chromatography uses a mobile phase of water and a stationary phase of paper?

    <p>paper chromatography</p> Signup and view all the answers

    What does a large Retardation Factor (Rf) in chromatography indicate?

    <p>Great solubility and small adsorption</p> Signup and view all the answers

    What is the principle behind centrifugation?

    <p>Separation based on density</p> Signup and view all the answers

    What does a small Retardation Factor (Rf) in chromatography indicate?

    <p>Small solubility and great adsorption</p> Signup and view all the answers

    What is the mobile phase in paper chromatography?

    <p>Water</p> Signup and view all the answers

    A photometer is device that measures?

    <p>light intensity</p> Signup and view all the answers

    What is the primary characteristic of anemia?

    <p>Low Hemoglobin levels</p> Signup and view all the answers

    Which component of the blood is primarily affected in anemia?

    <p>Red Blood Cells</p> Signup and view all the answers

    Which of the following best describes plasma?

    <p>The liquid, cell-free part of blood treated with anti-coagulants</p> Signup and view all the answers

    What is the primary difference between plasma and serum?

    <p>Plasma is anti-coagulated while serum is clotted</p> Signup and view all the answers

    What is the key characteristic that distinguishes serum from plasma?

    <p>Serum is the liquid part of blood after coagulation, devoid of clotting factors</p> Signup and view all the answers

    What causes the suspended particles to collect as a pellet in the tube bottom during centrifugation?

    <p>Centrifugal force</p> Signup and view all the answers

    What is the primary force responsible for separating molecules based on their molecular weight in a centrifuge?

    <p>Centrifugal force</p> Signup and view all the answers

    What does the hematocrit value of 45% of total volume of centrifuged blood represent?

    <p>The combined volume of red cells and buffy coat</p> Signup and view all the answers

    In a centrifuged blood sample, what does the 'buffy coat' mainly consist of?

    <p>White blood cells and platelets</p> Signup and view all the answers

    What does a photometer primarily measure?

    <p>Light intensity</p> Signup and view all the answers

    What is the hemoglobin concentration for females ?

    <p>14+ 2 g/dL</p> Signup and view all the answers

    What property of a sample can be determined using a photometer?

    <p>Light absorption in specific wavelength</p> Signup and view all the answers

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