32 Questions
Which physical properties can be used to separate benzene, phenol, and aniline?
Molecular weight, boiling point, freezing point
What is the purpose of preparative chromatography?
To purify enough of a substance
What is the principle on which chromatography is usually based?
Partition of solute between two phases
Which technique is used when the mobile phase is a gas?
Gas chromatography
What distinguishes the reactivity of phenol and aniline towards another reagent?
-OH and NH2 functional groups
What is the purpose of analytical chromatography?
To determine the chemical composition of a sample
In liquid chromatography, what is the main factor responsible for the separation of a sample into its individual parts?
The interactions of the sample with the mobile and stationary phases
What is the most popular chromatography technique for separating a mixture in liquid chromatography?
Liquid solid column chromatography
In paper chromatography, what forms the stationary phase?
The aqueous component of the solvent binding to cellulose paper
What is the principle of paper chromatography?
Separating and identifying both colored and colorless mixtures
What is the main factor responsible for the separation of a sample in liquid solid column chromatography?
The interactions of the sample with the mobile and stationary phases
Which type of paper chromatography involves the solvent running up the paper by capillary action?
Ascending Paper Chromatography
What component of the solvent forms the mobile phase in paper chromatography?
The organic component migrating through the paper
Which factor plays a key role in determining the effluent concentration in a chromatogram?
The retention time or volume when a solute exits the injector and passes through the column and detector
What does liquid solid column chromatography feature as its mobile phase?
A liquid mobile phase slowly filtering down through a solid stationary phase
In liquid chromatography, what determines effluent concentration in a chromatogram?
The retention time or volume when a solute exits the injector and passes through the column and detector
Chromatography is a laboratory technique used for mixing substances together.
False
The principle of chromatography is based on the partition of solute between two phases.
True
Gas chromatography uses a liquid mobile phase.
False
Paper chromatography involves the solvent running down the paper by capillary action.
False
Analytical chromatography is used to purify enough of a substance.
False
Physical properties such as molecular weight and boiling point cannot be used to separate benzene, phenol, and aniline.
False
Liquid chromatography is a technique used to separate a sample into its individual parts, and this separation occurs based on the interactions of the sample with the mobile phase only.
False
In liquid solid column chromatography, the mobile phase slowly filters up through the solid stationary phase, bringing the separated components with it.
False
Ascending paper chromatography involves the solvent running down the paper by capillary action.
False
In paper chromatography, the aqueous component of the solvent forms the mobile phase.
False
Paper chromatography is a technique used to separate a sample into its individual parts based on the interactions of the sample with the stationary phase only.
False
The retention time or volume in chromatography is when a solute enters the injector and passes through the column and the detector.
True
Liquid solid column chromatography is classified based on the physical states of the mobile and stationary phases.
False
The solvent in paper chromatography binds to the cellulose paper to form the stationary phase.
True
Paper chromatography can be used to separate and identify both colored and colorless mixtures.
True
Liquid chromatography is a technique used to separate a sample into its individual parts based on the interactions of the sample with only the stationary phase.
False
Learn how to separate benzene, phenol, and aniline using chromatographic techniques based on their distinct physical and chemical properties. Understand the criteria for separation including molecular weight, boiling point, freezing point, crystallization, solubility, and density.
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