Introduction to Biochemical Engineering

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Questions and Answers

What best describes the primary focus of biochemical engineering?

  • Focusing exclusively on plant biology
  • Developing computer models for biochemical reactions
  • Executing biological processes on an industrial scale (correct)
  • Creating solely chemical processes

Which of the following is NOT a component utilized in bioprocessing?

  • Animal cells
  • Microbial cells
  • Plant cells
  • Physical chemicals (correct)

Bioprocessing plays a crucial role in which of the following industries?

  • Pharmaceutical (correct)
  • Transportation
  • Finance
  • Telecommunications

What is one potential application of bioprocessing in waste management?

<p>Producing alcohol and biofuels (A)</p> Signup and view all the answers

How has the field of biochemical engineering changed in recent years?

<p>It has gained increasing relevance due to biotechnology (B)</p> Signup and view all the answers

Which statement about bioprocessing is accurate?

<p>It can create useful products while also managing waste. (C)</p> Signup and view all the answers

Which of the following describes an example of bioprocessing application?

<p>Production of therapeutic chemicals (A)</p> Signup and view all the answers

What do biochemical engineers primarily provide between biology and chemical engineering?

<p>A practical link (B)</p> Signup and view all the answers

What does modern biotechnology allow the manipulation of?

<p>Genetic material of individual cells (B)</p> Signup and view all the answers

What is the main purpose of cell fusion in biotechnology?

<p>To combine desirable characteristics from different cell types (A)</p> Signup and view all the answers

What is a primary application of hybridoma technology?

<p>Producing useful antibodies (C)</p> Signup and view all the answers

Which statement best describes bioprocessing technologies?

<p>It requires a large-scale process to be economically efficient. (B)</p> Signup and view all the answers

Which of the following is NOT a characteristic of modern biotechnology?

<p>Only involves traditional farming methods (D)</p> Signup and view all the answers

What is the main benefit of biosynthetic insulin over earlier sources of insulin?

<p>It is derived from genetically engineered bacteria. (A)</p> Signup and view all the answers

What was the source of insulin before the development of biosynthetic versions?

<p>Pancreas from pigs and cattle. (D)</p> Signup and view all the answers

Which of the following processes utilizes biofuels derived from superorganisms?

<p>Removal of pollutants from water. (A)</p> Signup and view all the answers

Which of the following is a key feature of functional foods?

<p>They promote health beyond basic nutrition. (D)</p> Signup and view all the answers

What advancement allowed for the first approval of human insulin as a genetically engineered product?

<p>Splicing of the rat insulin gene into E. coli. (C)</p> Signup and view all the answers

What significant risk is associated with individuals suffering from Type 1 diabetes?

<p>Poor chance of normal life expectancy. (C)</p> Signup and view all the answers

How do computers based on biological molecules differ from traditional silicon-based computers?

<p>They utilize DNA rather than silicon chips. (B)</p> Signup and view all the answers

What role do fermented foods and beverages play in health?

<p>They can contribute to gut health and digestion. (C)</p> Signup and view all the answers

What role do raw materials play in biological processes?

<p>They provide nutrients and energy sources. (C)</p> Signup and view all the answers

What is considered an advantage of biological processes over traditional chemical processes?

<p>Mild reaction conditions. (A)</p> Signup and view all the answers

Which of the following is NOT a component of a bioreactor?

<p>Separation processes (A)</p> Signup and view all the answers

What is one of the process development sciences utilized in fermentation?

<p>Microbiology (C)</p> Signup and view all the answers

In the context of fermentation, which statement accurately describes the process?

<p>It involves the enzymatic transformation of organic compounds. (A)</p> Signup and view all the answers

What is a disadvantage associated with biological processes?

<p>Potential for environmental contamination. (C)</p> Signup and view all the answers

Which option correctly reflects the nature of enzyme-catalyzed reactions in biological processes?

<p>They have a high degree of specificity. (A)</p> Signup and view all the answers

What defines the concept of 'fermentation' in modern industry?

<p>The enzymatic transformation of organic compounds in a controlled environment. (D)</p> Signup and view all the answers

What does the variable kxE0 relate to in enzyme kinetics?

<p>The maximum velocity of enzymatic reaction, Vmax (D)</p> Signup and view all the answers

In a Continuous Stirred-Tank Reactor (CSTR), what happens to dCs/dt at steady-state?

<p>It approaches zero. (A)</p> Signup and view all the answers

Which condition is NOT an assumption of a batch reactor?

<p>The system reaches equilibrium quickly. (A)</p> Signup and view all the answers

Which plot can be used to analyze the change of CS over time in a batch reactor?

<p>(CS0-CS)/ln(CS0/CS) vs. t (A)</p> Signup and view all the answers

How is the initial reaction rate related to enzyme activity?

<p>It should be computed in terms of how much enzyme was used. (A)</p> Signup and view all the answers

What do the kinetic parameters in enzyme kinetics refer to?

<p>They reflect the rate of product formation. (B)</p> Signup and view all the answers

What characteristic of an ideal reactor is highlighted in the content regarding a packed bed reactor?

<p>It can effectively mimic multi-staged reactors. (D)</p> Signup and view all the answers

What is the initial step to set up the rate equation in enzyme kinetics?

<p>Determine the rate limiting step. (B)</p> Signup and view all the answers

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Study Notes

Introduction to Biochemical Engineering

  • It involves conducting large-scale biological processes
  • Bridging biological sciences with chemical engineering
  • Growing importance due to recent developments in biotechnology

Bioprocessing Applications

  • Its applications are essential in industries such as food, chemicals, and pharmaceuticals
  • It leverages microbial, animal, and plant cells and their components to produce new products
  • Also includes destroying harmful waste
  • Key applications include: pharmaceuticals and medicines, organ growth, food production, bio-based computers, and industrial processes like pollution clean-up

Biotechnology

  • Traditional biotechnology refers to the use of living organisms or their components to produce or modify products, often associated with improving crops and livestock.
  • Modern biotechnology includes:
    • Recombinant DNA technology: manipulating genetic material to develop organisms that produce new products
    • Cell fusion technology: combining cells to create hybrid cells with desirable characteristics

Bioprocessing Technologies

  • Large-scale bioprocesses require a technologically feasible and economically efficient design
  • Key components include:
    • Biomass (nutrients and energy sources)
    • Medium (liquid mixture of growth factors)
    • Cell Culture: pure strain (microorganisms, animal or plant cells, or enzyme catalysts)
    • Bioreactor/Fermenter: controls fermentation with components such as optimal design, operation, and control
    • Product Recovery and Purification: ensures maximum purity with minimal cost
  • Development and design rely on:
    • Basic sciences: microbiology, biochemistry, molecular biology, genetics
    • Reaction kinetics
    • Process control
    • Separation processes

Biological Processes

  • Their key advantages include:
    • Mild reaction conditions
    • Specificity
    • Effectiveness
    • Renewable resources
  • Their key disadvantages include:
    • Complex product mixtures
    • Dilute aqueous environments
    • Contamination
    • Variability

Fermentation

  • A process for production of alcohol or lactic acid from glucose (traditional definition)
  • Originally referred to the metabolism of an organic compound under anaerobic conditions, now encompasses both aerobic and anaerobic large-scale culture of organisms

Enzyme Reactor with Simple Kinetics

  • Assumes well-mixed batch reactor contents
  • Steady-state Plug-Flow Reactor (PFR) properties are constant with time
  • Three reactor types:
    • Batch Reactor
    • Steady-State Plug-Flow Reactor (PFR)
    • Continuous Stirred-Tank Reactor (CSTR)

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