Intro to Microbiology: Scope and Microbes

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Questions and Answers

Which of the following is NOT a typical characteristic of bacteria?

  • Reproduction through asexual methods
  • Presence of membrane-bound nucleus (correct)
  • Unicellular organization
  • Prokaryotic cell structure

Which of the following best describes the role of pseudopodia in protozoa?

  • Propelling the organism through the environment.
  • Removing waste products from the cell.
  • Cell extensions of amoebas that flow for locomotion. (correct)
  • Converting carbon dioxide to oxygen.

Which of the following is a primary difference between bacteria and archaea?

  • Archaea reproduce sexually, while bacteria reproduce asexually.
  • Bacteria have cell walls containing peptidoglycan, while archaea do not. (correct)
  • Bacteria are typically found in extreme environements, while archaea are not.
  • Archaea are eukaryotic, while bacteria are prokaryotic.

How did Ignaz Semmelweis contribute to the prevention of infection and disease?

<p>He discovered the disease-fighting power of handwashing. (B)</p> Signup and view all the answers

Which of the following statements accurately describes viruses?

<p>They consist of a DNA or RNA genome enclosed in a protein coat. (C)</p> Signup and view all the answers

Which of the following properties is commonly associated with prokaryotic cells?

<p>lack of membrane-enclosed organelles (D)</p> Signup and view all the answers

Which cellular structure is responsible for the characteristic acid-fastness in Mycobacterium species?

<p>The presence of mycolic acid in the cell wall (C)</p> Signup and view all the answers

Which term refers to the ability of a bacterial cell to move or propel itself through its environment?

<p>Motility (D)</p> Signup and view all the answers

Based on their function, which cellular part is considered the 'Mitochondria of Prokaryotic Cells'?

<p>cytoplasmic membrane (D)</p> Signup and view all the answers

Under which of the following biosafety levels would work involving agents that can cause serious or lethal disease through respiratory transmission typically be conducted?

<p>BSL-3 (D)</p> Signup and view all the answers

Which statement best explains the purpose of Koch's postulates?

<p>They are a set of criteria used to establish a causative link between a microorganism and a disease. (B)</p> Signup and view all the answers

What is the primary reason acid is used in the acid-fast staining procedure?

<p>To decolorize non-acid-fast bacteria. (D)</p> Signup and view all the answers

Which of the following methods is best suited for sterilizing heat-sensitive liquids?

<p>Filtration (A)</p> Signup and view all the answers

In the Gram stain procedure, what is the function of safranin?

<p>It serves as a counterstain to stain Gram-negative bacteria. (B)</p> Signup and view all the answers

If a bacterium is described as capnophilic, what environmental condition does it require for optimal growth?

<p>Increased carbon dioxide levels (A)</p> Signup and view all the answers

What is the main purpose of using indicator organisms in autoclaves?

<p>To verify effective sterilization. (A)</p> Signup and view all the answers

How temperature is related to microorganism classification?

<p>Microorganisms are classified as psychrophilic, mesophilic, or thermophilic based on temperature. (A)</p> Signup and view all the answers

Which of the following statements describes the primary component and purpose of bacterial capsules?

<p>The protective layer which protects bacteria against phagocytosis. (D)</p> Signup and view all the answers

What characteristic is tested for in an acid-fast stain?

<p>mycolic acids (D)</p> Signup and view all the answers

What is the role of an inoculating loop in microbiology?

<p>To transfer microorganisms from one medium to another. (B)</p> Signup and view all the answers

During bacterial conjugation, what structure is essential for transferring genetic material between cells?

<p>pili (B)</p> Signup and view all the answers

During which growth phase in a bacterial population does sporulation typically occur?

<p>Stationary phase (B)</p> Signup and view all the answers

What type of relationship exists when Lactobacillus acidophilus produces beneficial outcomes to the host, in the vagina?

<p>Mutualism (B)</p> Signup and view all the answers

Which method can indicate the success for pasturization?

<p>phosphatase (A)</p> Signup and view all the answers

Based on what basis are parasitic bacteria categorized?

<p>use of tissue damage (C)</p> Signup and view all the answers

Aside from a disease symptom, what does 'pili' contribute

<p>adhering bacterial cells to the host (C)</p> Signup and view all the answers

What happens with 2 step or serial dilutions

<p>The organism has created new spores (C)</p> Signup and view all the answers

An organism, that in the process becomes injured, best describes

<p>under the appropriate environmental state (B)</p> Signup and view all the answers

What should be implemented to increase the results of an acid fast result?

<p>add mordant (B)</p> Signup and view all the answers

A species that is not requiring oxygen at all can best be known as

<p>strict anaerobes (C)</p> Signup and view all the answers

When an infection doesn't affect a person, and no noticable signs, what is the best descriptive word

<p>a subclinical stage (C)</p> Signup and view all the answers

Which of the statements best describes 'incidence'?

<p>measures new cases. (C)</p> Signup and view all the answers

When the autoclave doesn't kill organisms, such as the priam, a protein what does that mean for the machines efficiency?

<p>not intended for the machine (D)</p> Signup and view all the answers

What indicates is one with a 'good' set of steps and sterilization at a specific test temp. for sterilization?

<p>Indicator at this point (C)</p> Signup and view all the answers

Which item would most often be a target by UV lamps?

<p>a working safety cabinet (B)</p> Signup and view all the answers

What indicates the O angtigen of the Salmonellae

<p>somatic cell (D)</p> Signup and view all the answers

Which of the following is not a way to demonstrate motility of microbes?

<p>Fluorescent microscope (D)</p> Signup and view all the answers

What does it mean to be an ‘obligate aerobe’?

<p>needs O2 always (D)</p> Signup and view all the answers

Microbial Growth that has changed, What type of consistency does this have?

<p>liquid (A)</p> Signup and view all the answers

Contribution of Microorganism to Cheese Products & Process depends on:

<p>the source of milk and the type (A)</p> Signup and view all the answers

If a BSL rating has caused 'a' number of problems because of transmissibility what should the BSL level be?

<p>BSL4 (A)</p> Signup and view all the answers

What is typically observed with a Mannitol results/effect when testing a 'selective differential media'?

<p>either is and is not dependent on the reactions going (A)</p> Signup and view all the answers

Which test does a color pink indicated on after there as a fast reaction involved in bacteria in some test?

<p>PPM rapid (A)</p> Signup and view all the answers

How often does one preform autoclave indicators check?

<p>every Week (A)</p> Signup and view all the answers

What is used in seroyping

<p>Vi Ag (C)</p> Signup and view all the answers

Flashcards

Microbiology

Study of living things too small to be seen without magnification

Microorganisms/Microbes

Microscopic organisms

Protozoa

Single-celled eukaryotes similar to animals in nutrient needs and cellular structure

Pseudopodia

Cell extensions of some protozoa that flow in the direction of travel

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Cilia

Numerous, short, hair-like protrusions that propel organisms through environment

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Flagella

Extensions of a cell that are fewer, longer, and more whip-like than cilia

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Algae

Unicellular or multicellular organisms that uses photosynthesis to create oxygen and energy

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Fungi

Eukaryotic organisms that obtains food from other organisms and possesses cell walls

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Molds

Multicellular fungi with hyphae that reproduce by sexual and asexual spores

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Yeast

Unicellular reproduce asexually by budding, some reproduce sexual spores

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Bacteria

Prokaryotes, unicellular, and lack nuclei. Cell walls usually contain peptidoglycan

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Louis Pasteur

Developed the Germ theory of disease. Developed vaccines against anthrax, rabies, and chicken cholera

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Robert Koch

Studied causative agents of disease, discovered Bacillus Anthracis, Mycobacterium Tuberculosis, and Vibrio Cholerae

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Binomial Nomenclature

The generic (GENUS) name followed by the species name.

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Taxonomy

Naming, Classifying, and Identifying Microorganisms

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Microbial nomenclature

Naming microorganisms. GENUS and Species.

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Prokaryotes

No nucleus. Instead, a nucleoid contains genetic material. Have 70s ribosomes, haploid.

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Capsule

Outer protective layer of the cell wall.

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Cell Wall

Defines the shape of the bacterial cell. Point of anchorage for flagella. Target for antibiotics.

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Cell Membrane

Transports nutrients. Metabolic energy production.

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Exotoxin

Is excreted by living bacteria and it does not require cell death for release, metabolic product of bacteria.

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Endotoxin

It requires cell death for release (cell wall disintegration), Composition: Polysaccharide and Lipids. effect =“Systemic.”Toxicity Low

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Exotoxin

Smaller. Usually present in a gram (+)is excreted by living bacteria and it does not require cell death for release

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Endotoxin

Size of the cell (usually produced by gram (-) organisms)

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Pathogenicity

Ability of a microbe to produce disease in a susceptible individual

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Virulence

Relative ability of a microorganism to cause disease or the degree of pathogenicity.

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Pili or Fimbriae

Hair-like structures for attachment

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Endospores

Resting cell, highly resistant to desiccation, heat, and chemical agents. Bacterial genera with spores: Bacillus and Clostridium

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Flagella

Organ for locomotion or motility. H antigen.

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Gram Positive (+)

Impermeable to alcohol, thick peptidoglycan With teichoic acid and exotoxin.

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Gram Negative (-)

Permeable to alcohol, thin peptidoglycan Has LPS (Lipopolysaccharide) - important in evading host defenses

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Incidence

The number of people in a population who develop a disease during a particular time period indicator of the spread of disease.

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Prevalence

Number of people in a population who have a disease at or during specified time; accounts old and new cases.

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Acute Disease

Develops rapidly but last only a short time (e.g., Influenza)

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Chronic Disease

Develops slowly (e.g., Tuberculosis)

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Incubation Period

Interval between initial infection and first appearance of any signs or symptoms

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Period of Illness

Shows signs and symtoms, E.g., Fever and chills, swollen lymph nodes, Gl disturbance - increase in WBC's

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Endotoxin

Usually produced by group (-)organisms. requires cell death for release (cell wall disintegration)

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Pasturization

Use to lower total bacterial count by 95-99% This method cannot eliminate bacterial endospores.

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Sterilization

removal of all microbial life

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Sanitization

Lowest the microbial count on eating of a sanitizing utensils

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Bacteriostasis

An antimicrobial that inhibits bacterial growth but does not kill the bacteria

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Bactericidal

An antimicrobial that kills a microorganism

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Wet (Moist) Heat

Autoclave acts by coagulating or denaturing enzymes and proteins

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Chemical Sterlization

Ethylene oxide (Gas Sterilant) - most commonly used chemical sterilant, used in gaseous form of sterilizing heat-sensitive objects.

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Ionizing Radiation

Uses short wavelength but high energy gamma rays.

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Antisepsis

Removes pathogens from living tissue

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Study Notes

Scopes of Microbiology

  • Microbiology is the study of living organisms too small to be seen without magnification.
  • Microorganisms can be seen through a microscope.

Microorganisms (Microbes)

  • These microscopic organisms are commonly called "germs, viruses, agents," but not all cause disease; usefulness and necessity to human life are also characteristics.
  • Normal flora is good bacteria.
  • Pathogens are bad bacteria.
  • Microbes are studied by microbiologists.

Different Types of Microorganisms

  • Bacteria are a type of microorganism.
  • Fungi are microorganisms.
  • Viruses are microorganisms.
  • Protozoa are microorganisms like parasites.

Classifying Microbes

  • Carolus Linnaeus created a system for grouping similar organisms.
  • Anton van Leeuwenhoek classified microorganisms into six categories: Fungi (mycology), Protozoa (protozoology), Algae (phycology), Bacteria (bacteriology), Archaea, and Small Animals.

Fungi

  • Eukaryotic organisms obtain food from other organisms and have cell walls.
  • Molds are multicellular fungi with hyphae.
  • Molds reproduce through sexual and asexual spores and can infect humans.
  • Yeasts are unicellular fungi.
  • Yeast reproduce asexually by budding and sometimes sexually.

Protozoa

  • Single-celled eukaryotes are similar to animals in nutrient needs and cellular structure.
  • Protozoa commonly live freely in water and some live in animal hosts.
  • Most protozoa reproduce asexually, but some reproduce sexually.
  • Many protozoa are capable of locomotion using pseudopodia, cilia, or flagella.
  • Pseudopodia are cell extensions of amoebas that flow in the direction of travel.
  • Amoebas can cause diarrhea.
  • Cilia are short, hair-like protrusions that propel organisms through the environment.
  • Flagella are extensions of a cell that are fewer, longer, and more whip-like than cilia.

Algae

  • Algae can be unicellular or multicellular.
  • Photosynthetic algae create oxygen and energy from carbon dioxide, sunlight, and water.
  • Algae is classified by pigmentation, storage products, and cell wall composition.
  • Examples of algae include pond scum, seaweeds, and giant kelp.

Bacteria and Archaea

  • Bacteria are prokaryotic microorganisms that are unicellular and lack nuclei.
  • Bacteria are much smaller than eukaryotes.
  • Bacteria are found where there is adequate moisture, and some thrive in extreme environments.
  • Bacteria typically reproduce asexually.
  • Bacteria cell walls contain peptidoglycan, though some lack cell walls.
  • Most bacteria are not disease-causing and can be beneficial.
  • Archaea have cell walls composed of polymers other than peptidoglycan.
  • Three major bacterial shapes exist.
    • Spiral
    • Bacilli
    • Cocci

Contributions of Louis Pasteur

  • Invented the Germ Theory of Disease.
  • Observed that bacteria, viruses, fungi, and protozoa all lead to many diseases
  • Vaccines for rabies and anthrax

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