Intrinsic Fluorescence of Polypeptides Quiz
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Questions and Answers

What type of light is used to excite tryptophan for fluorescence?

  • UV-vis light (correct)
  • Infrared light
  • Visible light
  • X-ray light

    • What change is commonly observed in denatured (unfolded) proteins' fluorescence spectrum?

  • No shift
  • Blue shift
  • Fluorescence quenching
  • Red shift (correct)

    • What amino acid gives fluorescence in addition to tryptophan?

  • Leucine
  • Cysteine
  • Glycine
  • Tyrosine (correct)

    • How can protein denaturation be monitored?

    <p>Via the tryptophan emission peak</p> Signup and view all the answers

    What is the range for observing the signal when monitoring structural changes using tryptophan fluorescence?

    <p>300-350 nm</p> Signup and view all the answers

    What is the main amino acid responsible for fluorescence in polypeptides?

    <p>Tyrosine</p> Signup and view all the answers

    What type of change is commonly observed in denatured (unfolded) proteins' fluorescence spectrum?

    <p>Red shift</p> Signup and view all the answers

    How can protein denaturation be monitored?

    <p>Via the Tryptophan Emission Peak</p> Signup and view all the answers

    What is the range for exciting tryptophan to observe fluorescence?

    <p>280-290 nm</p> Signup and view all the answers

    What is the Tryptophan Emission Spectrum sensitive to?

    <p>Its environment</p> Signup and view all the answers

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