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Questions and Answers
What type of light is used to excite tryptophan for fluorescence?
What type of light is used to excite tryptophan for fluorescence?
- UV-vis light (correct)
- Infrared light
- Visible light
- X-ray light
What change is commonly observed in denatured (unfolded) proteins' fluorescence spectrum?
What change is commonly observed in denatured (unfolded) proteins' fluorescence spectrum?
- No shift
- Blue shift
- Fluorescence quenching
- Red shift (correct)
What amino acid gives fluorescence in addition to tryptophan?
What amino acid gives fluorescence in addition to tryptophan?
- Leucine
- Cysteine
- Glycine
- Tyrosine (correct)
How can protein denaturation be monitored?
How can protein denaturation be monitored?
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What is the range for observing the signal when monitoring structural changes using tryptophan fluorescence?
What is the range for observing the signal when monitoring structural changes using tryptophan fluorescence?
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What is the main amino acid responsible for fluorescence in polypeptides?
What is the main amino acid responsible for fluorescence in polypeptides?
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What type of change is commonly observed in denatured (unfolded) proteins' fluorescence spectrum?
What type of change is commonly observed in denatured (unfolded) proteins' fluorescence spectrum?
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How can protein denaturation be monitored?
How can protein denaturation be monitored?
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What is the range for exciting tryptophan to observe fluorescence?
What is the range for exciting tryptophan to observe fluorescence?
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What is the Tryptophan Emission Spectrum sensitive to?
What is the Tryptophan Emission Spectrum sensitive to?
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