Intravital Staining Techniques

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Questions and Answers

What is the main purpose of the supravital method?

To stain dead cells

To examine living cells (correct)

To remove tissue from the body

To produce a permanent stain

The regressive staining method intensifies the color of specific cell structures at the expense of obscuring details.

True

What is the best vital dye mentioned in the content?

Neutral Red

Methylene Blue N is used to demonstrate ____________.

reticulocytes Signup and view all the answers

Match the following dyes with their specific usage:

Neutral Red = Best vital dye Janus Green = For mitochondria Methylene Blue N = Demonstrates reticulocytes Cyanine = General staining Signup and view all the answers

Which of the following statements about the dye uptake is true?

Dyes stay in the tissue once taken up. Signup and view all the answers

What is the primary purpose of Trypan Blue in vital staining?

To stain only the cytoplasm of living cells Signup and view all the answers

The nucleus is generally resistant to vital stains like Trypan Blue.

True Signup and view all the answers

What must be done to a 1 gram concentration of Trypan Blue for use?

Dissolve it in 100 ml of sterile distilled water. Signup and view all the answers

The use of specific dyes that differentiate particular substances based on color is known as __________ staining.

metachromatic Signup and view all the answers

Match the following techniques or materials with their definitions:

Vital Staining = Selective staining of living cell constituents Supravital Staining = Staining that occurs after cell death Metachromatic Staining = Dyes giving different colors to specific substances Chromotrope = Chemical that alters dye color through polymerization Signup and view all the answers

Which of the following statements about Trypan Blue is true?

It is toxic to living cells if not used immediately. Signup and view all the answers

What does it indicate if nuclear structures are demonstrated during vital staining?

Permeability of the membrane to the dye, indicating cell death. Signup and view all the answers

Supravital staining is performed by injecting the dye into the living organism.

True Signup and view all the answers

What is the primary challenge associated with progressive staining?

It can lead to obscured results due to diffusion. Signup and view all the answers

Progressive staining is more favored than regressive staining.

False Signup and view all the answers

Name one common dye used in progressive staining.

Janus Green B Signup and view all the answers

Intravital staining involves injecting staining solutions into parts of a ______ body.

animal Signup and view all the answers

Match the following dyes with their specific usage in staining:

Lithium = Common dye used for staining Carmine = Common dye used for staining India Ink = Common dye used for vital staining Janus Green B = Demonstrates Kupffer cells Signup and view all the answers

What method involves staining tissue elements in a predetermined sequence?

Progressive staining Signup and view all the answers

Study Notes

Supravital Method

Involves staining living cells extracted from an animal's body.
Staining is performed until the desired intensity is achieved.
Demonstrates reticulocytes using Methylene Blue N.
Common dyes:
- Neutral Red - considered the best vital dye.
- Janus Green - utilized for staining mitochondria.

Intravital Method

Involves injecting staining solutions directly into specific parts of an animal's body.
Demonstrates Kupffer cells in the liver with intravenous injection of Janus Green B.
Common dyes include:
- Lithium.
- Carmine.
- India Ink.
- Trypan Blue.

Progressive Staining

Dyes are applied in a chronological sequence.
Less favored due to challenges in achieving strong staining without affecting surrounding tissues.
Results can be diffused and obscure due to insufficient intense staining.

Vital Staining

Selective staining targeting living cell constituents, primarily the cytoplasm.
The nucleus is generally resistant to vital stains, indicating cell viability.
If nuclear structures are visible during vital staining, it suggests cell membrane permeability to the dye, indicating cell death prior to staining.
Stains living cells via direct dye injection at various body sites, administered intravenously, intraperitoneally, or subcutaneously.

Metachromatic Staining

Utilizes specific dyes to differentiate substances by providing a distinct color from that of the dye itself.
Involves the substance Chromotrope, found in certain tissues.
Chromotrope has the ability to polymerize dye molecules, altering the dye's color.
Present in Babes Ernst granules of C.

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Description

Explore the methods of intravital staining used to visualize specific cells within tissue sections. This quiz focuses on the progressive staining technique and its application in demonstrating liver Kupffer cells through intravenous dye administration.