Intestinal and Extraintestinal Amebas & Flagellates
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Questions and Answers

______ spp. cysts are identified using cultured, Hematoxylin, and Eosin stains.

Acanthamoeba

______ trophozoites are cultured and stained with trichrome and Pap’s stain.

Entamoeba gingivalis

The cysts of Giardia intestinalis can be identified using Wet Mount (WM) with Iodine, ______, Trichrome, and Wet Mount under Differential Interference Contrast (DIC).

Trichrome

Chilomastix mesnili cysts can be diagnosed using Wet Mount (WM) with Iodine and ______.

<p>Trichrome</p> Signup and view all the answers

______ cysts are diagnosable via Iron-hematoxylin and Trichrome staining.

<p>E.hominis</p> Signup and view all the answers

Retortamonas intestinalis cysts are exclusively identifiable using ______ staining techniques.

<p>Trichrome</p> Signup and view all the answers

While most intestinal ameba trophozoites are stained using trichrome, the first trophozoite of ______ is an exception.

<p>E.histolytica</p> Signup and view all the answers

______ trophozoites are identified through culture methods.

<p>Naegleria fowleri</p> Signup and view all the answers

Flashcards

Concentration Wet Mount (CWM)

A method for identifying parasites, involving concentration and staining, used for organisms like E.Histolytica and E.Coli.

Trichrome Stain

Staining technique used to differentiate cellular components, aiding in parasite identification for organisms like E.Histolytica and E.Coli.

Wet Mount with Iodine

A staining method used for visualizing parasites in fecal samples, aiding identification. Used for organisms like E.Nana and I.Butschlii.

Culturing Amoebas

Method used to grow and study Naegleria fowleri and Acanthamoeba spp.

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Hematoxylin and Eosin (H&E)

A permanent staining method used to examine cells and tissues, often used alongside culturing techniques for organisms like Acanthamoeba spp.

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Pap's Stain

A stain used in cytology to study cells, useful for identifying organisms like Entamoeba gingivalis.

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Iron-Hematoxylin Stain

A staining technique that uses iron to bind to certain cellular structures, enhancing their visibility under a microscope; useful in identifying organisms like E.hominis.

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WM under DIC

A microscopy technique that enhances contrast in transparent specimens without staining; useful for observing organisms like G.intestinalis.

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Study Notes

  • The images provided show cysts and trophozoites of intestinal and extraintestinal amebas and flagellates, stained with various methods for identification.

Cysts of Intestinal Amebas

  • Includes images of E. histolytica, E. hartmanni, E. coli, E. polecki, E. nana, and I. butschlii.
  • Staining methods used are concentrated wet mount, wet mount with iodine, trichrome, and CWM with iodine.

Trophozoites of Intestinal Amebas

  • Contains images of E. histolytica, E. hartmanni, E. coli, E. polecki, E. nana, and I. butschlii.
  • Staining methods are CWM with iodine and trichrome.
  • All are stained with trichrome except the first troph of E. histolytica.

Cysts of Extraintestinal Amebas

  • Features images of Naegleria fowleri and Acanthamoeba spp.
  • Staining methods are culturing and hematoxylin and eosin.

Trophozoites of Extraintestinal Amebas

  • Encompasses images of Entamoeba gingivalis, Naegleria fowleri, and Acanthamoeba spp.
  • Staining methods are culturing, trichrome, and Pap's stain.

Cysts of Intestinal Flagellates

  • Includes images of G. intestinalis, C. mesnili, E. hominis, and R. intestinalis.
  • Staining methods are wet mount with iodine, iron-hematoxylin, trichrome, and wet mount under DIC (differential interference contrast).

Trophozoites of Intestinal Flagellates

  • Contains images of G. intestinalis, C. mesnili, D. fragilis, T. hominis, E hominis, and R. intestinalis.
  • Staining Methods include wet mount with iodine, trichrome, Kohn stain, and Giemsa.

Trophozoites of Extraintestinal Flagellates

  • Includes images of T. tenax and T. vaginalis.
  • Staining Method is Giemsa.

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Description

Images of cysts and trophozoites of intestinal and extraintestinal amebas and flagellates, stained with various methods for identification. Includes E. histolytica, E. hartmanni, E. coli, E. polecki, E. nana, I. butschlii, Naegleria fowleri and Acanthamoeba spp.

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