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Interpreting Hemolysis Test Results

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Questions and Answers

What is the likely cause of incompatible results with post-transfusion samples but compatible with pre-transfusion samples?

Presence of antibodies in the recipient's blood
Intrinsic red cell defect
Bacterial contamination of the donor unit
Anamnestic antibody response (correct)

What is the next step in investigation if both crossmatches are compatible but there is strong clinical evidence of acute immune hemolytic reaction?

Stop the transfusion immediately
Perform antibody detection tests using more sensitive techniques (correct)
Administer steroids to the patient
Repeat the crossmatch

What is the purpose of examining post-transfusion urine for the presence of free hemoglobin or hemosiderin?

To diagnose acute kidney injury
To detect hemolysis (correct)
To assess blood transfusion reaction
To monitor kidney function

Why is it important to measure serum haptoglobin in both pre and post transfusion samples?

To assess hemolysis (A) Signup and view all the answers

What is the purpose of testing the donor unit for the corresponding antigen?

To identify the antigen responsible for the reaction (B) Signup and view all the answers

What is indicated by a negative hemolysis test and a negative DAT?

An acute immune hemolytic reaction has NOT occurred (D) Signup and view all the answers

What is the next step in investigation if there is a discrepancy in patient/donor identification?

Search appropriate records and notify physician-in-charge (D) Signup and view all the answers

What is the interpretation of a positive hemolysis test and/or a positive DAT?

Suggests the presence of coating antibodies in circulating cells (D) Signup and view all the answers

What is the purpose of repeating ABO/RH typing and antibody screening in further investigation?

To identify errors in labeling and/or identification (C) Signup and view all the answers

What is the result of incompatible crossmatch with both pre and post transfusion samples against the sample of the donor?

Error during pre-transfusion testing (D) Signup and view all the answers

What is the significance of antibody complement-coated cells being rapidly destroyed?

It indicates that an acute immune hemolytic reaction has NOT occurred (B) Signup and view all the answers

What is the primary purpose of the autocontrol test in an investigation of incompatible crossmatch?

To determine if the problem is due to either blood group isoantibody or nonspecific reaction (C) Signup and view all the answers

What is the potential cause of a positive reaction in the saline at 37°C stage of investigation?

Irregular antibody, autoagglutinin, or rouleaux formation (A) Signup and view all the answers

What is the importance of grading donor reactions in the investigation of suspected hemolytic transfusion reaction?

To determine if the reactions of incompatible blood are all of the same strength (C) Signup and view all the answers

What is the purpose of rechecking the Rh type in the investigation of incompatible crossmatch?

To confirm the correct selection of blood unit (A) Signup and view all the answers

What is the significance of a positive DAT result in the investigation of suspected hemolytic transfusion reaction?

It indicates the presence of irregular antibody (D) Signup and view all the answers

What is the primary step in the investigation of incompatible crossmatch?

Verifying the patient's identity (C) Signup and view all the answers

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