Podcast
Questions and Answers
What is the minimum fixation time for small tissue specimens?
What is the minimum fixation time for small tissue specimens?
- At least 24 hours
- At least 1 hour
- At least 12 hours
- At least 6 hours (correct)
Which of the following is NOT an essential clinical information requirement on a requisition?
Which of the following is NOT an essential clinical information requirement on a requisition?
- Surgical procedure details
- Patient history
- Billing information (correct)
- Specimen site
What should be done if a specimen's fixation time is less than the required duration for medium to large specimens?
What should be done if a specimen's fixation time is less than the required duration for medium to large specimens?
- Consider the specimen unusable
- Immediately process the specimen
- Move the specimen to the refrigerator
- Delay processing and inform anatomic pathology ROD (correct)
How long are tissue and fluid specimens stored after processing?
How long are tissue and fluid specimens stored after processing?
Who is permitted to load and operate the tissue processing machine?
Who is permitted to load and operate the tissue processing machine?
What is the consequence of insufficient fixation time for a specimen?
What is the consequence of insufficient fixation time for a specimen?
What is the recommended minimum fixation time for medium to large tissue specimens?
What is the recommended minimum fixation time for medium to large tissue specimens?
What is a consequence of a requisition lacking physician details?
What is a consequence of a requisition lacking physician details?
What type of water should be used to fill the tissue flotation bath on Wednesdays?
What type of water should be used to fill the tissue flotation bath on Wednesdays?
What is the minimum duration for which tissues are placed in Bucket 1 of the tissue processor?
What is the minimum duration for which tissues are placed in Bucket 1 of the tissue processor?
Which reagent is used in the first two buckets of tissue processing?
Which reagent is used in the first two buckets of tissue processing?
What is the correct temperature setting for the flotation bath during the morning routine?
What is the correct temperature setting for the flotation bath during the morning routine?
What is the recommended method to remove metallic sheen from Hematoxylin dye?
What is the recommended method to remove metallic sheen from Hematoxylin dye?
How long are tissues treated with Absolute Alcohol in buckets 5, 6, and 7?
How long are tissues treated with Absolute Alcohol in buckets 5, 6, and 7?
How should blocks be organized on the base of the oven after being unloaded from the tissue processor?
How should blocks be organized on the base of the oven after being unloaded from the tissue processor?
What is the purpose of using control blocks in tissue processing?
What is the purpose of using control blocks in tissue processing?
What is used as a clearing agent in the tissue processing procedure?
What is used as a clearing agent in the tissue processing procedure?
When filling the embedding mold with wax, what should be done once the wax slightly overflows?
When filling the embedding mold with wax, what should be done once the wax slightly overflows?
What must be done after the molds cool down in the freezer for ten minutes?
What must be done after the molds cool down in the freezer for ten minutes?
During the Hematoxylin and Eosin staining procedure, how long are tissues rinsed in tap water?
During the Hematoxylin and Eosin staining procedure, how long are tissues rinsed in tap water?
Which of the following steps is NOT part of the embedding procedure?
Which of the following steps is NOT part of the embedding procedure?
In the Papanicolau staining procedure, which alcohol concentration is used first?
In the Papanicolau staining procedure, which alcohol concentration is used first?
What is a critical step to ensure proper adhesion of the tissue block to the cassette during embedding?
What is a critical step to ensure proper adhesion of the tissue block to the cassette during embedding?
What is the duration for which tissues are exposed to Harris Hematoxylin during the Hematoxylin and Eosin staining?
What is the duration for which tissues are exposed to Harris Hematoxylin during the Hematoxylin and Eosin staining?
What is the purpose of ammonia water in the staining procedure?
What is the purpose of ammonia water in the staining procedure?
Which bucket of the tissue processor uses absolute alcohol for the maximum time?
Which bucket of the tissue processor uses absolute alcohol for the maximum time?
Flashcards
Inadequate clinical information
Inadequate clinical information
Incomplete or missing clinical information in a requisition form, such as patient history, surgical procedure details, or specimen site, which hinders proper interpretation.
Lack of physician or clinician details
Lack of physician or clinician details
An absence of information regarding the requesting physician or surgeon, potentially critical for special processing instructions.
Fixation Time for Small Specimens
Fixation Time for Small Specimens
Minimum fixation time for small tissue specimens (less than 1 cm) for adequate preservation.
Fixation Time for Medium to Large Specimens
Fixation Time for Medium to Large Specimens
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Dehydration, Clearing, and Infiltration
Dehydration, Clearing, and Infiltration
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Tissue Processing Machine
Tissue Processing Machine
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Fixation Protocols
Fixation Protocols
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Anatomic Pathology ROD
Anatomic Pathology ROD
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Tissue Processing
Tissue Processing
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Tissue Processor
Tissue Processor
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Embedding
Embedding
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Tissue Flotation Bath
Tissue Flotation Bath
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Sectioning
Sectioning
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Staining
Staining
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Pap's Stain
Pap's Stain
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H&E Stain
H&E Stain
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Tissue Processing Control Block
Tissue Processing Control Block
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Tissue Processor Bucket
Tissue Processor Bucket
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Dehydration
Dehydration
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Clearing
Clearing
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Infiltration
Infiltration
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Deparaffinization
Deparaffinization
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Xylene
Xylene
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Hematoxylin and Eosin (H&E) Staining
Hematoxylin and Eosin (H&E) Staining
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Papanicolaou (Pap) Staining
Papanicolaou (Pap) Staining
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Hematoxylin
Hematoxylin
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Study Notes
Histopathology and Cytopathology Interns Duties and Responsibilities
-
Morning Routine:
- Fill the tissue flotation bath with tap water (Monday-Tuesday-Thursday-Friday-Sunday) or distilled water (Wednesday-Saturday).
- Set the temperature to 45-50°C. Ensure the orange light is on.
- Fill the bath ¾ full, leaving ½ inch of space at the top.
- Plug in the hot plate and ensure the orange light is on.
- Check Pap's Stain and H&E stain reagent levels to ensure enough to submerge slides being stained.
- Fill Coplin jars ¾ full with stains.
- Fill staining jars slightly less than half full with stains.
- Remove metallic sheen from Hematoxylin dye (due to oxidation). Press carbon paper on the dye before filtering.
- Filter Hematoxylin and Eosin stains with flower filter paper.
- Always replace tap water containers.
- Set up embedding area, lay out scrap paper.
-
Tissue Embedding:
- Arrange blocks in order, based on accession number from the loading logbook, at the base of the oven.
- Double-check cassette and filter paper numbers; ask staff if inconsistencies arise.
- Place tissue blocks on the hot plate and embedding molds.
- Pour paraffin wax, ensuring tissues are close together but not overlapping.
- Freeze the bottom of the mold using ice blocks.
- Hold specimens in place with a teasing needle or forceps.
- Place the molds in the blood bank freezer for 10 minutes. (10 blocks at a time).
- Detach blocks from molds and trim the edges.
- Arrange blocks on an ice block in order for staff to start cutting.
-
Cutting:
- Pre-label slides with block arrangement per loading logbook. Double-check numbers.
- Allow water to drain from slides.
- Clean hot plate with paper blot surface until excess paraffin is absorbed.
- Ask staff on duty if the first 10 slides produced can proceed on the hotplate for 10 minutes.
-
Staining:
- Remove slide rack after 15 minutes in the oven.
- Proceed with staining procedure (Neoclear step 1).
- Ensure all slides are submerged in all staining stations.
- Do not let slides dry during staining.
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Description
This quiz covers the essential duties and responsibilities of interns in the histopathology and cytopathology settings. Participants will learn about the morning routine tasks, tissue embedding procedures, and proper maintenance of staining reagents. It's essential for those preparing for real-world applications in medical laboratories.
