Intern Duties in Histopathology

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Questions and Answers

What is the minimum fixation time for small tissue specimens?

At least 24 hours

At least 1 hour

At least 12 hours

At least 6 hours (correct)

Which of the following is NOT an essential clinical information requirement on a requisition?

Surgical procedure details

Patient history

Billing information (correct)

Specimen site

What should be done if a specimen's fixation time is less than the required duration for medium to large specimens?

Consider the specimen unusable

Immediately process the specimen

Move the specimen to the refrigerator

Delay processing and inform anatomic pathology ROD (correct)

How long are tissue and fluid specimens stored after processing?

One month or until after the surgery audit Signup and view all the answers

Who is permitted to load and operate the tissue processing machine?

Only histopathology rotating staff Signup and view all the answers

What is the consequence of insufficient fixation time for a specimen?

Processing must be delayed, and the ROD must be informed Signup and view all the answers

What is the recommended minimum fixation time for medium to large tissue specimens?

At least 12 hours Signup and view all the answers

What is a consequence of a requisition lacking physician details?

It may lead to delays in processing due to special instructions Signup and view all the answers

What type of water should be used to fill the tissue flotation bath on Wednesdays?

Distilled water Signup and view all the answers

What is the minimum duration for which tissues are placed in Bucket 1 of the tissue processor?

1 hour Signup and view all the answers

Which reagent is used in the first two buckets of tissue processing?

10% Neutral Buffered Formalin Signup and view all the answers

What is the correct temperature setting for the flotation bath during the morning routine?

45-50℃ Signup and view all the answers

What is the recommended method to remove metallic sheen from Hematoxylin dye?

Pressing carbon paper onto the staining rack Signup and view all the answers

How long are tissues treated with Absolute Alcohol in buckets 5, 6, and 7?

1 hour Signup and view all the answers

How should blocks be organized on the base of the oven after being unloaded from the tissue processor?

By increasing accession number Signup and view all the answers

What is the purpose of using control blocks in tissue processing?

To assess tissue processing after reagent change Signup and view all the answers

What is used as a clearing agent in the tissue processing procedure?

Xylene Signup and view all the answers

When filling the embedding mold with wax, what should be done once the wax slightly overflows?

Proceed to place the mold on ice immediately Signup and view all the answers

What must be done after the molds cool down in the freezer for ten minutes?

Detach the block and trim the mold edge Signup and view all the answers

During the Hematoxylin and Eosin staining procedure, how long are tissues rinsed in tap water?

2 changes Signup and view all the answers

Which of the following steps is NOT part of the embedding procedure?

Apply a heat treatment at 70℃ before embedding Signup and view all the answers

In the Papanicolau staining procedure, which alcohol concentration is used first?

95% alcohol Signup and view all the answers

What is a critical step to ensure proper adhesion of the tissue block to the cassette during embedding?

Ensuring tissues are close but not overlapping Signup and view all the answers

What is the duration for which tissues are exposed to Harris Hematoxylin during the Hematoxylin and Eosin staining?

2 minutes Signup and view all the answers

What is the purpose of ammonia water in the staining procedure?

To enhance staining intensity Signup and view all the answers

Which bucket of the tissue processor uses absolute alcohol for the maximum time?

Bucket 7 Signup and view all the answers

Signup and view all the answers

Study Notes

Histopathology and Cytopathology Interns Duties and Responsibilities

Morning Routine:
- Fill the tissue flotation bath with tap water (Monday-Tuesday-Thursday-Friday-Sunday) or distilled water (Wednesday-Saturday).
- Set the temperature to 45-50°C. Ensure the orange light is on.
- Fill the bath ¾ full, leaving ½ inch of space at the top.
- Plug in the hot plate and ensure the orange light is on.
- Check Pap's Stain and H&E stain reagent levels to ensure enough to submerge slides being stained.
- Fill Coplin jars ¾ full with stains.
- Fill staining jars slightly less than half full with stains.
- Remove metallic sheen from Hematoxylin dye (due to oxidation). Press carbon paper on the dye before filtering.
- Filter Hematoxylin and Eosin stains with flower filter paper.
- Always replace tap water containers.
- Set up embedding area, lay out scrap paper.
Tissue Embedding:
- Arrange blocks in order, based on accession number from the loading logbook, at the base of the oven.
- Double-check cassette and filter paper numbers; ask staff if inconsistencies arise.
- Place tissue blocks on the hot plate and embedding molds.
- Pour paraffin wax, ensuring tissues are close together but not overlapping.
- Freeze the bottom of the mold using ice blocks.
- Hold specimens in place with a teasing needle or forceps.
- Place the molds in the blood bank freezer for 10 minutes. (10 blocks at a time).
- Detach blocks from molds and trim the edges.
- Arrange blocks on an ice block in order for staff to start cutting.
Cutting:
- Pre-label slides with block arrangement per loading logbook. Double-check numbers.
- Allow water to drain from slides.
- Clean hot plate with paper blot surface until excess paraffin is absorbed.
- Ask staff on duty if the first 10 slides produced can proceed on the hotplate for 10 minutes.
Staining:
- Remove slide rack after 15 minutes in the oven.
- Proceed with staining procedure (Neoclear step 1).
- Ensure all slides are submerged in all staining stations.
- Do not let slides dry during staining.

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Description

This quiz covers the essential duties and responsibilities of interns in the histopathology and cytopathology settings. Participants will learn about the morning routine tasks, tissue embedding procedures, and proper maintenance of staining reagents. It's essential for those preparing for real-world applications in medical laboratories.