Podcast
Questions and Answers
What are the key nutrients required for bacteria to multiply quickly?
What are the key nutrients required for bacteria to multiply quickly?
Carbohydrates, proteins, minerals, and vitamins
Why is the maximum allowed temperature for bacterial growth in a school lab typically set at 25°C?
Why is the maximum allowed temperature for bacterial growth in a school lab typically set at 25°C?
Above this temperature, more harmful pathogens are likely to grow.
In what two forms can bacteria be grown for study?
In what two forms can bacteria be grown for study?
Nutrient broth solution or as colonies on an agar gel plate
Why is it vital to grow uncontaminated cultures of microorganisms in the lab?
Why is it vital to grow uncontaminated cultures of microorganisms in the lab?
What is the purpose of aseptic techniques in microbiology?
What is the purpose of aseptic techniques in microbiology?
How does temperature affect the growth rate of bacteria?
How does temperature affect the growth rate of bacteria?
What is the aim of investigating bacterial growth using agar plates and measuring zones of inhibition?
What is the aim of investigating bacterial growth using agar plates and measuring zones of inhibition?
What technique is used to place filter paper discs soaked in antiseptics/antibiotics onto agar plates containing bacteria?
What technique is used to place filter paper discs soaked in antiseptics/antibiotics onto agar plates containing bacteria?
Why is preventing contamination important in microbiology investigations?
Why is preventing contamination important in microbiology investigations?
Which bacterial cultures are commonly used in practicals involving investigating bacterial growth?
Which bacterial cultures are commonly used in practicals involving investigating bacterial growth?
What is the purpose of calculating the area of each zone of inhibition?
What is the purpose of calculating the area of each zone of inhibition?
What is the significance of using uncontaminated agar plates in the investigation of bacterial growth?
What is the significance of using uncontaminated agar plates in the investigation of bacterial growth?
Why is it important to only lift the lid of the petri dish a little when working with bacterial cultures?
Why is it important to only lift the lid of the petri dish a little when working with bacterial cultures?
Why is calculating the area of an inhibition zone a more accurate way of comparing the effect of different substances on bacterial growth?
Why is calculating the area of an inhibition zone a more accurate way of comparing the effect of different substances on bacterial growth?
What equation is used to calculate the area of an inhibition zone?
What equation is used to calculate the area of an inhibition zone?
Why is it more accurate to measure the diameter of an inhibition zone rather than the radius?
Why is it more accurate to measure the diameter of an inhibition zone rather than the radius?
What is the purpose of taking at least two measurements of the diameter of an inhibition zone?
What is the purpose of taking at least two measurements of the diameter of an inhibition zone?
Why is it necessary to half the diameter measurement before using the area equation to calculate the inhibition zone area?
Why is it necessary to half the diameter measurement before using the area equation to calculate the inhibition zone area?
