In Vitro Fertilization Overview
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Questions and Answers

What is the purpose of using pre-implantation embryo biopsies?

They are used to assess the embryo's genetic characteristics before transfer.

What are the risks associated with multiple births from IVF?

Multiple births increase the risks of early labor and low birth weight.

How does maternal age affect miscarriage rates in IVF?

Miscarriage rates rise with increasing maternal age in IVF pregnancies.

What complications can arise from the egg retrieval process in IVF?

<p>Possible complications include bleeding, inflammation, or damage to nearby organs.</p> Signup and view all the answers

What is ovarian hyperstimulation syndrome?

<p>It is a condition where the ovaries become swollen and painful due to fertility drugs.</p> Signup and view all the answers

What does ectopic pregnancy mean in the context of IVF?

<p>Ectopic pregnancy occurs when the fertilized egg implants outside the uterus.</p> Signup and view all the answers

Why are stem cells considered unspecialized cells?

<p>They have the potential to develop into many different specialized cell types.</p> Signup and view all the answers

What factor primarily influences the risk of birth defects, regardless of conception method?

<p>Maternal age is the main risk factor for the development of birth defects.</p> Signup and view all the answers

What are the two types of culture media used in embryo culture?

<p>Single culture medium and sequential culture medium.</p> Signup and view all the answers

Which components enhance embryonic growth and development in artificial culture media?

<p>Nucleotides, amino acids, vitamins, and cholesterol.</p> Signup and view all the answers

What criteria do embryologists use to grade embryos during selection?

<p>Number of cells, evenness of growth, and degree of fragmentation.</p> Signup and view all the answers

What morphological scoring system is used in embryo selection?

<p>A system that optimizes pregnancy rates based on embryo morphology.</p> Signup and view all the answers

When are embryos typically transferred after retrieval?

<p>Embryos are transferred 3 days after retrieval.</p> Signup and view all the answers

What is one advantage of transferring embryos at the blastocyst stage?

<p>It results in higher pregnancy rates.</p> Signup and view all the answers

What determines the number of embryos transferred to the patient's uterus?

<p>The number available, the age of the woman, and health factors.</p> Signup and view all the answers

How are the best embryos transferred to the uterus?

<p>Using a thin plastic catheter that goes through the cervix.</p> Signup and view all the answers

What are microbial pesticides and how do they control pests?

<p>Microbial pesticides consist of naturally occurring or genetically controlled microorganisms that control pests by producing toxins, causing diseases, or preventing other microorganisms.</p> Signup and view all the answers

Explain what Plant-Incorporated Protectants (PIPs) are.

<p>Plant-Incorporated Protectants (PIPs) are pesticides produced from genetic material added to the plant, enabling the plant to manufacture its pest control substance.</p> Signup and view all the answers

What role do biochemical pesticides play in pest control?

<p>Biochemical pesticides are naturally occurring substances that control pests through nontoxic mechanisms, such as disrupting mating with insect sex pheromones.</p> Signup and view all the answers

Identify a widely used microbial pesticide and its target pests.

<p>A widely used microbial pesticide is Bacillus thuringiensis (Bt), which targets caterpillars, some fly larvae, and some beetle larvae.</p> Signup and view all the answers

What is the mechanism of action for the strain Bt var.kurstaki?

<p>Bt var.kurstaki is toxic to lepidopteran larvae, such as butterflies, skippers, and moths.</p> Signup and view all the answers

Describe how the neem plant extract functions as a biopesticide.

<p>Neem extract disrupts the life cycle of pests and is effective against several insects like rice cutworm and tobacco caterpillar.</p> Signup and view all the answers

Discuss how man-made pheromones are utilized in pest management.

<p>Man-made pheromones disrupt insect mating by creating confusion during their search for mates or by attracting male insects to traps.</p> Signup and view all the answers

How do biochemical pesticides differ from traditional pesticides?

<p>Biochemical pesticides differ from traditional pesticides by using naturally occurring substances and nontoxic mechanisms for pest control.</p> Signup and view all the answers

What type of cells can be stimulated to form insulin-producing cells for transplantation?

<p>Human embryonic stem cells.</p> Signup and view all the answers

What is Parkinson's disease characterized by?

<p>Muscular weakness, rigidity, and a fine, slowly spreading tremor.</p> Signup and view all the answers

What type of neurons are lost in Parkinson's disease?

<p>Dopaminergic neurons.</p> Signup and view all the answers

What is a benefit of using stem cells in treating brain diseases?

<p>They can be developed into new neurons for transplantation.</p> Signup and view all the answers

What are biopesticides derived from?

<p>Natural materials like plants, bacteria, fungi, and minerals.</p> Signup and view all the answers

List two advantages of biopesticides over conventional pesticides.

<p>They pose fewer risks and break down more quickly, leading to less pollution.</p> Signup and view all the answers

What is a characteristic feature of biopesticides regarding their action against pests?

<p>They suppress rather than eliminate pest populations.</p> Signup and view all the answers

How do the application times of biopesticides differ from conventional pesticides?

<p>Biopesticides have relatively critical application times.</p> Signup and view all the answers

What is the purpose of using GnRH agonists in the lengthy protocol?

<p>GnRH agonists are used for down regulation of the pituitary ovarian axis.</p> Signup and view all the answers

How long does the injection of gonadotropins typically last in the short protocol?

<p>Typically, the injection lasts for about ten days.</p> Signup and view all the answers

What role does FSH play in the ovarian stimulation process?

<p>FSH is used for hyperstimulation of the ovaries to develop follicles.</p> Signup and view all the answers

What is the significance of monitoring oestradiol levels during stimulation?

<p>Monitoring oestradiol levels helps assess the development of ovarian follicles.</p> Signup and view all the answers

What surgical procedure is performed to remove eggs from the ovaries?

<p>The procedure performed is called follicular aspiration.</p> Signup and view all the answers

What hormone is injected to induce the final maturation of eggs?

<p>Human chorionic gonadotropin (hCG) is injected for final maturation.</p> Signup and view all the answers

What effect do GnRH antagonists have during the last days of stimulation?

<p>GnRH antagonists prevent spontaneous ovulation during stimulation.</p> Signup and view all the answers

What is the main goal of the ovary stimulation protocols?

<p>The main goal is to trigger the development of multiple follicles in the ovaries.</p> Signup and view all the answers

What is in vitro fertilization (IVF)?

<p>IVF is a type of assistive reproductive technology that involves retrieving eggs from a woman's ovaries and fertilizing them with sperm.</p> Signup and view all the answers

What is the purpose of the stimulation step in IVF?

<p>The stimulation step aims to induce super ovulation, allowing the production of multiple eggs during one menstrual cycle.</p> Signup and view all the answers

List the basic five steps involved in IVF.

<p>The five steps are: Stimulation, Egg retrieval, Insemination and fertilization, Embryo culture, and Embryo selection and embryo transfer.</p> Signup and view all the answers

What are fertility drugs and their role in IVF?

<p>Fertility drugs are medications prescribed to stimulate the ovaries to produce multiple eggs during a cycle.</p> Signup and view all the answers

On what day of the menstrual cycle is treatment generally started for IVF?

<p>Treatment cycles for IVF are generally started on the third day of menstruation.</p> Signup and view all the answers

What is an embryo in the context of IVF?

<p>An embryo is a fertilized egg that is transferred to a woman's uterus during the IVF process.</p> Signup and view all the answers

What conditions might make IVF advisable?

<p>IVF may be advisable in cases of tubal disease, unexplained infertility, endometriosis, and male factor infertility.</p> Signup and view all the answers

Why is the production of multiple eggs important in IVF?

<p>Multiple eggs increase the likelihood of developing viable embryos for successful implantation.</p> Signup and view all the answers

Study Notes

In Vitro Fertilization and Embryo Transfer

  • IVF is a type of assistive reproductive technology (ART)
  • It involves retrieving eggs from a woman's ovaries and fertilizing them with sperm
  • The fertilized egg, called an embryo, is then transferred to the woman's uterus
  • This procedure can assist with fertility issues or prevent genetic problems
  • It's beneficial for tubal disease, endometriosis, unexplained infertility, male factor infertility, and failed induction.

Steps of IVF

  • Step 1: Stimulation:

    • Also known as super ovulation
    • Requires multiple eggs for increased embryo viability
    • Fertility drugs (exogenous gonadotropins) stimulate multiple egg production
    • Treatment cycles usually start on the third day of menstruation.
    • Regular transvaginal ultrasounds and blood tests to monitor hormone levels are necessary
    • Two main protocols for stimulation: lengthy (GnRH agonist) and short (injectable gonadotropins)
  • Step 2: Egg Retrieval:

    • Also known as follicular aspiration
    • Minor surgical procedure to remove eggs
    • Final maturation is induced by hCG injection, which causes ovulation
    • Eggs are retrieved 24-36 hours after hCG injection
  • Step 3: Insemination and Fertilization:

    • Selection of the best quality embryos
    • Sperm washing to remove inactive cells and seminal fluids
    • Sperm incubation with the egg for fertilization
    • Intracytoplasmic sperm injection (ICSI) may be used in cases of low sperm count or motility
  • Step 4: Embryo Culture:

    • Embryos are cultured for 3 days after retrieval
    • Culture can be performed in artificial culture medium or autologous endometrial co-culture
    • Media for artificial culture typically contains glucose, pyruvate, and energy-supplying components
  • Step 5: Embryo Selection and Transfer:

    • Embryo selection based on cell number, growth, and fragmentation
    • High-quality embryos are chosen to maximize pregnancy rates
    • Transferred to the woman's uterus using a thin plastic catheter
    • Depends on number of embryos available, woman's age, and other factors.

Risks of IVF

  • Multiple Births: Increased risk of premature birth and low birth weight
  • Premature Birth and Low Body Weight: Babies born from IVF may be born earlier than normal
  • Ovarian Hyperstimulation Syndrome (OHSS): Ovaries swell and become painful due to fertility drugs
  • Miscarriage: Similar rates to natural pregnancies, especially in older women
  • Ectopic Pregnancy: Fertilized egg implants outside the uterus, which is not viable
  • Birth Defects: Further research needed to determine if IVF is associated with an increased risk

Stem Cell Technology

  • Stem Cells: Primitive, unspecialized cells that can develop into various specialized cell types
  • Stem Cell Applications: Heart disease, diabetes, arthritis, brain disease, eye diseases
  • Diabetes: Stem cell therapy may be used to normalize glucose and insulin levels in diabetic patients
  • Parkinson's Disease: Stem cells can be grown into new brain cells, replacing damaged cells, and used to test new drugs.

Biopesticides

  • Biopesticides: Derived from natural materials (plants, bacteria, fungi, viruses)
  • Types: Microbial and plant-incorporated protectants (PIPs)
  • Microbial Biopesticides: Naturally occurring microorganisms that control pests
  • PIPs: Plants genetically modified to produce pest-controlling substances
  • Biochemical Biopesticides: Naturally occurring substances that disrupt insect mating or control pests via pheromones.

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Description

This quiz covers the essentials of In Vitro Fertilization (IVF) and the steps involved in the process. Learn about the retrieval of eggs, fertilization, and embryo transfer, as well as the various fertility issues IVF can address. Test your understanding of the significant protocols used in IVF treatment.

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