In Vitro Fertilization Overview
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Questions and Answers

What is the purpose of using pre-implantation embryo biopsies?

They are used to assess the embryo's genetic characteristics before transfer.

What are the risks associated with multiple births from IVF?

Multiple births increase the risks of early labor and low birth weight.

How does maternal age affect miscarriage rates in IVF?

Miscarriage rates rise with increasing maternal age in IVF pregnancies.

What complications can arise from the egg retrieval process in IVF?

<p>Possible complications include bleeding, inflammation, or damage to nearby organs.</p> Signup and view all the answers

What is ovarian hyperstimulation syndrome?

<p>It is a condition where the ovaries become swollen and painful due to fertility drugs.</p> Signup and view all the answers

What does ectopic pregnancy mean in the context of IVF?

<p>Ectopic pregnancy occurs when the fertilized egg implants outside the uterus.</p> Signup and view all the answers

Why are stem cells considered unspecialized cells?

<p>They have the potential to develop into many different specialized cell types.</p> Signup and view all the answers

What factor primarily influences the risk of birth defects, regardless of conception method?

<p>Maternal age is the main risk factor for the development of birth defects.</p> Signup and view all the answers

What are the two types of culture media used in embryo culture?

<p>Single culture medium and sequential culture medium.</p> Signup and view all the answers

Which components enhance embryonic growth and development in artificial culture media?

<p>Nucleotides, amino acids, vitamins, and cholesterol.</p> Signup and view all the answers

What criteria do embryologists use to grade embryos during selection?

<p>Number of cells, evenness of growth, and degree of fragmentation.</p> Signup and view all the answers

What morphological scoring system is used in embryo selection?

<p>A system that optimizes pregnancy rates based on embryo morphology.</p> Signup and view all the answers

When are embryos typically transferred after retrieval?

<p>Embryos are transferred 3 days after retrieval.</p> Signup and view all the answers

What is one advantage of transferring embryos at the blastocyst stage?

<p>It results in higher pregnancy rates.</p> Signup and view all the answers

What determines the number of embryos transferred to the patient's uterus?

<p>The number available, the age of the woman, and health factors.</p> Signup and view all the answers

How are the best embryos transferred to the uterus?

<p>Using a thin plastic catheter that goes through the cervix.</p> Signup and view all the answers

What are microbial pesticides and how do they control pests?

<p>Microbial pesticides consist of naturally occurring or genetically controlled microorganisms that control pests by producing toxins, causing diseases, or preventing other microorganisms.</p> Signup and view all the answers

Explain what Plant-Incorporated Protectants (PIPs) are.

<p>Plant-Incorporated Protectants (PIPs) are pesticides produced from genetic material added to the plant, enabling the plant to manufacture its pest control substance.</p> Signup and view all the answers

What role do biochemical pesticides play in pest control?

<p>Biochemical pesticides are naturally occurring substances that control pests through nontoxic mechanisms, such as disrupting mating with insect sex pheromones.</p> Signup and view all the answers

Identify a widely used microbial pesticide and its target pests.

<p>A widely used microbial pesticide is Bacillus thuringiensis (Bt), which targets caterpillars, some fly larvae, and some beetle larvae.</p> Signup and view all the answers

What is the mechanism of action for the strain Bt var.kurstaki?

<p>Bt var.kurstaki is toxic to lepidopteran larvae, such as butterflies, skippers, and moths.</p> Signup and view all the answers

Describe how the neem plant extract functions as a biopesticide.

<p>Neem extract disrupts the life cycle of pests and is effective against several insects like rice cutworm and tobacco caterpillar.</p> Signup and view all the answers

Discuss how man-made pheromones are utilized in pest management.

<p>Man-made pheromones disrupt insect mating by creating confusion during their search for mates or by attracting male insects to traps.</p> Signup and view all the answers

How do biochemical pesticides differ from traditional pesticides?

<p>Biochemical pesticides differ from traditional pesticides by using naturally occurring substances and nontoxic mechanisms for pest control.</p> Signup and view all the answers

What type of cells can be stimulated to form insulin-producing cells for transplantation?

<p>Human embryonic stem cells.</p> Signup and view all the answers

What is Parkinson's disease characterized by?

<p>Muscular weakness, rigidity, and a fine, slowly spreading tremor.</p> Signup and view all the answers

What type of neurons are lost in Parkinson's disease?

<p>Dopaminergic neurons.</p> Signup and view all the answers

What is a benefit of using stem cells in treating brain diseases?

<p>They can be developed into new neurons for transplantation.</p> Signup and view all the answers

What are biopesticides derived from?

<p>Natural materials like plants, bacteria, fungi, and minerals.</p> Signup and view all the answers

List two advantages of biopesticides over conventional pesticides.

<p>They pose fewer risks and break down more quickly, leading to less pollution.</p> Signup and view all the answers

What is a characteristic feature of biopesticides regarding their action against pests?

<p>They suppress rather than eliminate pest populations.</p> Signup and view all the answers

How do the application times of biopesticides differ from conventional pesticides?

<p>Biopesticides have relatively critical application times.</p> Signup and view all the answers

What is the purpose of using GnRH agonists in the lengthy protocol?

<p>GnRH agonists are used for down regulation of the pituitary ovarian axis.</p> Signup and view all the answers

How long does the injection of gonadotropins typically last in the short protocol?

<p>Typically, the injection lasts for about ten days.</p> Signup and view all the answers

What role does FSH play in the ovarian stimulation process?

<p>FSH is used for hyperstimulation of the ovaries to develop follicles.</p> Signup and view all the answers

What is the significance of monitoring oestradiol levels during stimulation?

<p>Monitoring oestradiol levels helps assess the development of ovarian follicles.</p> Signup and view all the answers

What surgical procedure is performed to remove eggs from the ovaries?

<p>The procedure performed is called follicular aspiration.</p> Signup and view all the answers

What hormone is injected to induce the final maturation of eggs?

<p>Human chorionic gonadotropin (hCG) is injected for final maturation.</p> Signup and view all the answers

What effect do GnRH antagonists have during the last days of stimulation?

<p>GnRH antagonists prevent spontaneous ovulation during stimulation.</p> Signup and view all the answers

What is the main goal of the ovary stimulation protocols?

<p>The main goal is to trigger the development of multiple follicles in the ovaries.</p> Signup and view all the answers

What is in vitro fertilization (IVF)?

<p>IVF is a type of assistive reproductive technology that involves retrieving eggs from a woman's ovaries and fertilizing them with sperm.</p> Signup and view all the answers

What is the purpose of the stimulation step in IVF?

<p>The stimulation step aims to induce super ovulation, allowing the production of multiple eggs during one menstrual cycle.</p> Signup and view all the answers

List the basic five steps involved in IVF.

<p>The five steps are: Stimulation, Egg retrieval, Insemination and fertilization, Embryo culture, and Embryo selection and embryo transfer.</p> Signup and view all the answers

What are fertility drugs and their role in IVF?

<p>Fertility drugs are medications prescribed to stimulate the ovaries to produce multiple eggs during a cycle.</p> Signup and view all the answers

On what day of the menstrual cycle is treatment generally started for IVF?

<p>Treatment cycles for IVF are generally started on the third day of menstruation.</p> Signup and view all the answers

What is an embryo in the context of IVF?

<p>An embryo is a fertilized egg that is transferred to a woman's uterus during the IVF process.</p> Signup and view all the answers

What conditions might make IVF advisable?

<p>IVF may be advisable in cases of tubal disease, unexplained infertility, endometriosis, and male factor infertility.</p> Signup and view all the answers

Why is the production of multiple eggs important in IVF?

<p>Multiple eggs increase the likelihood of developing viable embryos for successful implantation.</p> Signup and view all the answers

Flashcards

In Vitro Fertilization (IVF)

A type of assisted reproductive technology where eggs are retrieved and fertilized with sperm outside the body.

Embryo

A fertilized egg.

Superovulation

Stimulating the ovaries to produce multiple eggs.

Egg retrieval

Collecting eggs from the woman's ovaries

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Fertility drugs

Medications that stimulate egg production.

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IVF Steps

Stimulation, egg retrieval, fertilization, embryo culture, embryo selection, and embryo transfer

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Assisted Reproductive Technology (ART)

Medical procedures to help people have children

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Embryo Transfer

Placing the fertilized embryo into the woman's uterus

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Fertility Medicine Stimulation Protocols

Methods to stimulate the ovaries for egg retrieval in fertility treatments.

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Lengthy Protocol

A fertility treatment protocol that suppresses the ovaries first, then stimulates them with hormones like FSH.

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Short Protocol

A fertility treatment protocol that skips the initial suppression phase and immediately stimulates the ovaries with hormones.

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GnRH Agonist

A medication that reduces the production of reproductive hormones, initially suppressing the ovaries, used in lengthy protocols.

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Follicle Stimulating Hormone (FSH)

A hormone used in fertility treatments to stimulate the growth of ovarian follicles that contain eggs.

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GnRH Antagonist

A drug that prevents the natural surge in LH, used to prevent premature ovulation during the final stimulation phase.

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Egg Retrieval (Follicular Aspiration)

Procedure to collect mature eggs from the ovaries.

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Human Chorionic Gonadotropin (hCG)

Hormone used to induce final egg maturation; mimics the role of LH.

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Sequential Culture Medium

Using different culture mediums at different stages of embryo development, for example, one medium for day 3, another for blastocyst stage.

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Dynamic Embryo Culture

Techniques that allow for fluid flow and embryo movement within the culture medium, mimicking natural conditions.

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Permeable Intrauterine Vessel

A new technology where embryos are encapsulated in a vessel that allows for nutrient and waste exchange, mimicking the natural environment.

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Embryo Grading

Evaluating embryos based on factors like number of cells, evenness of growth, and fragmentation.

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HLA-G

A protein found in embryos that can be considered a secondary parameter for selection, especially when evaluating embryos with similar morphological qualities.

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Blastocyst Transfer

Transferring embryos to the uterus at the blastocyst stage, a later stage of embryo development.

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Multiple Pregnancies

Risk associated with transferring multiple embryos, which clinics and regulatory bodies aim to minimize.

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Catheter

A thin plastic tube used to transfer embryos into the woman's uterus.

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Pre-implantation embryo biopsy

A procedure where cells are removed from a developing embryo before it implants in the uterus, allowing for genetic testing.

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Multiple births in IVF

The chance of having twins, triplets, or more when multiple embryos are transferred to the uterus during IVF.

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Ovarian hyperstimulation syndrome (OHSS)

A condition caused by fertility drugs that stimulates the ovaries, leading to swollen, painful ovaries.

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Miscarriage in IVF

The loss of a pregnancy before 20 weeks, occurring at similar rates in IVF and naturally conceived pregnancies.

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Complications of egg retrieval

Potential risks associated with the procedure to collect eggs from the ovaries, including bleeding, damage to organs, and complications from anesthesia.

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Ectopic pregnancy in IVF

A condition where the fertilized egg implants outside the uterus, typically in the fallopian tube, leading to a non-viable pregnancy.

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Stem cells (potential)

Cells with the ability to develop into many different cell types, considered the raw material for building various tissues and organs.

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Stem cell specialization

The process where stem cells differentiate and become specialized cells with specific functions, like muscle cells or blood cells.

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Stem Cell Therapy for Diabetes

Using stem cells grown in a lab to create insulin-producing cells, which are then transplanted into a diabetic patient to help regulate blood sugar levels.

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Parkinson's Disease Cause

A chronic neurological disorder caused by the loss of dopamine-producing neurons in the brain, leading to tremors, rigidity, and movement problems.

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Stem Cell Therapy for Parkinson's

Treatment for Parkinson's disease where stem cells are grown into dopamine-producing neurons and transplanted into the brain to replace the damaged cells.

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Umbilical Cord Stem Cells

Stem cells present in the umbilical cord after birth, capable of repairing damaged cells.

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Biopesticides

Pesticides derived from natural sources like plants, bacteria, fungi, and minerals, offering a safer alternative to synthetic chemicals.

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Biopesticides Benefits

Biopesticides provide effective pest control, promote human and environmental safety, and minimize pollution.

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Biopesticides vs. Conventional

Biopesticides are generally more targeted and specific in their action, decompose faster, and have lower environmental impact than conventional chemical pesticides.

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Biopesticides Characteristics

Biopesticides act slowly, have specific application times, and suppress rather than eliminate pests, with limited persistence and a short shelf life.

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Microbial Pesticides

Pesticides containing microorganisms, like bacteria, fungi, or viruses, that can kill or suppress targeted pests.

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Plant-Incorporated-Protectants (PIPs)

Pesticides produced by plants genetically modified to contain pest-killing substances.

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Biochemical Pesticides

Pesticides derived from naturally occurring substances that control pests by non-toxic mechanisms, such as interfering with mating or attracting pests to traps.

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Bacillus thuringiensis (Bt)

A soil-dwelling bacterium that produces proteins toxic to certain insect larvae.

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Bt var. kurstaki

A specific strain of Bacillus thuringiensis toxic to lepidopteran (butterfly, skipper, and moth) larvae.

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Bt var. aizawai

A specific strain of Bacillus thuringiensis toxic to wax moth larvae.

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Bt var. israelensis

A specific strain of Bacillus thuringiensis toxic to mosquito, midge, fungus gnats, and blackfly larvae.

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Study Notes

In Vitro Fertilization and Embryo Transfer

  • IVF is a type of assistive reproductive technology (ART)
  • It involves retrieving eggs from a woman's ovaries and fertilizing them with sperm
  • The fertilized egg, called an embryo, is then transferred to the woman's uterus
  • This procedure can assist with fertility issues or prevent genetic problems
  • It's beneficial for tubal disease, endometriosis, unexplained infertility, male factor infertility, and failed induction.

Steps of IVF

  • Step 1: Stimulation:

    • Also known as super ovulation
    • Requires multiple eggs for increased embryo viability
    • Fertility drugs (exogenous gonadotropins) stimulate multiple egg production
    • Treatment cycles usually start on the third day of menstruation.
    • Regular transvaginal ultrasounds and blood tests to monitor hormone levels are necessary
    • Two main protocols for stimulation: lengthy (GnRH agonist) and short (injectable gonadotropins)
  • Step 2: Egg Retrieval:

    • Also known as follicular aspiration
    • Minor surgical procedure to remove eggs
    • Final maturation is induced by hCG injection, which causes ovulation
    • Eggs are retrieved 24-36 hours after hCG injection
  • Step 3: Insemination and Fertilization:

    • Selection of the best quality embryos
    • Sperm washing to remove inactive cells and seminal fluids
    • Sperm incubation with the egg for fertilization
    • Intracytoplasmic sperm injection (ICSI) may be used in cases of low sperm count or motility
  • Step 4: Embryo Culture:

    • Embryos are cultured for 3 days after retrieval
    • Culture can be performed in artificial culture medium or autologous endometrial co-culture
    • Media for artificial culture typically contains glucose, pyruvate, and energy-supplying components
  • Step 5: Embryo Selection and Transfer:

    • Embryo selection based on cell number, growth, and fragmentation
    • High-quality embryos are chosen to maximize pregnancy rates
    • Transferred to the woman's uterus using a thin plastic catheter
    • Depends on number of embryos available, woman's age, and other factors.

Risks of IVF

  • Multiple Births: Increased risk of premature birth and low birth weight
  • Premature Birth and Low Body Weight: Babies born from IVF may be born earlier than normal
  • Ovarian Hyperstimulation Syndrome (OHSS): Ovaries swell and become painful due to fertility drugs
  • Miscarriage: Similar rates to natural pregnancies, especially in older women
  • Ectopic Pregnancy: Fertilized egg implants outside the uterus, which is not viable
  • Birth Defects: Further research needed to determine if IVF is associated with an increased risk

Stem Cell Technology

  • Stem Cells: Primitive, unspecialized cells that can develop into various specialized cell types
  • Stem Cell Applications: Heart disease, diabetes, arthritis, brain disease, eye diseases
  • Diabetes: Stem cell therapy may be used to normalize glucose and insulin levels in diabetic patients
  • Parkinson's Disease: Stem cells can be grown into new brain cells, replacing damaged cells, and used to test new drugs.

Biopesticides

  • Biopesticides: Derived from natural materials (plants, bacteria, fungi, viruses)
  • Types: Microbial and plant-incorporated protectants (PIPs)
  • Microbial Biopesticides: Naturally occurring microorganisms that control pests
  • PIPs: Plants genetically modified to produce pest-controlling substances
  • Biochemical Biopesticides: Naturally occurring substances that disrupt insect mating or control pests via pheromones.

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Description

This quiz covers the essentials of In Vitro Fertilization (IVF) and the steps involved in the process. Learn about the retrieval of eggs, fertilization, and embryo transfer, as well as the various fertility issues IVF can address. Test your understanding of the significant protocols used in IVF treatment.

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