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Questions and Answers
What is the purpose of using pre-implantation embryo biopsies?
What is the purpose of using pre-implantation embryo biopsies?
They are used to assess the embryo's genetic characteristics before transfer.
What are the risks associated with multiple births from IVF?
What are the risks associated with multiple births from IVF?
Multiple births increase the risks of early labor and low birth weight.
How does maternal age affect miscarriage rates in IVF?
How does maternal age affect miscarriage rates in IVF?
Miscarriage rates rise with increasing maternal age in IVF pregnancies.
What complications can arise from the egg retrieval process in IVF?
What complications can arise from the egg retrieval process in IVF?
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What is ovarian hyperstimulation syndrome?
What is ovarian hyperstimulation syndrome?
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What does ectopic pregnancy mean in the context of IVF?
What does ectopic pregnancy mean in the context of IVF?
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Why are stem cells considered unspecialized cells?
Why are stem cells considered unspecialized cells?
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What factor primarily influences the risk of birth defects, regardless of conception method?
What factor primarily influences the risk of birth defects, regardless of conception method?
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What are the two types of culture media used in embryo culture?
What are the two types of culture media used in embryo culture?
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Which components enhance embryonic growth and development in artificial culture media?
Which components enhance embryonic growth and development in artificial culture media?
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What criteria do embryologists use to grade embryos during selection?
What criteria do embryologists use to grade embryos during selection?
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What morphological scoring system is used in embryo selection?
What morphological scoring system is used in embryo selection?
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When are embryos typically transferred after retrieval?
When are embryos typically transferred after retrieval?
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What is one advantage of transferring embryos at the blastocyst stage?
What is one advantage of transferring embryos at the blastocyst stage?
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What determines the number of embryos transferred to the patient's uterus?
What determines the number of embryos transferred to the patient's uterus?
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How are the best embryos transferred to the uterus?
How are the best embryos transferred to the uterus?
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What are microbial pesticides and how do they control pests?
What are microbial pesticides and how do they control pests?
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Explain what Plant-Incorporated Protectants (PIPs) are.
Explain what Plant-Incorporated Protectants (PIPs) are.
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What role do biochemical pesticides play in pest control?
What role do biochemical pesticides play in pest control?
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Identify a widely used microbial pesticide and its target pests.
Identify a widely used microbial pesticide and its target pests.
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What is the mechanism of action for the strain Bt var.kurstaki?
What is the mechanism of action for the strain Bt var.kurstaki?
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Describe how the neem plant extract functions as a biopesticide.
Describe how the neem plant extract functions as a biopesticide.
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Discuss how man-made pheromones are utilized in pest management.
Discuss how man-made pheromones are utilized in pest management.
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How do biochemical pesticides differ from traditional pesticides?
How do biochemical pesticides differ from traditional pesticides?
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What type of cells can be stimulated to form insulin-producing cells for transplantation?
What type of cells can be stimulated to form insulin-producing cells for transplantation?
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What is Parkinson's disease characterized by?
What is Parkinson's disease characterized by?
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What type of neurons are lost in Parkinson's disease?
What type of neurons are lost in Parkinson's disease?
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What is a benefit of using stem cells in treating brain diseases?
What is a benefit of using stem cells in treating brain diseases?
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What are biopesticides derived from?
What are biopesticides derived from?
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List two advantages of biopesticides over conventional pesticides.
List two advantages of biopesticides over conventional pesticides.
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What is a characteristic feature of biopesticides regarding their action against pests?
What is a characteristic feature of biopesticides regarding their action against pests?
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How do the application times of biopesticides differ from conventional pesticides?
How do the application times of biopesticides differ from conventional pesticides?
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What is the purpose of using GnRH agonists in the lengthy protocol?
What is the purpose of using GnRH agonists in the lengthy protocol?
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How long does the injection of gonadotropins typically last in the short protocol?
How long does the injection of gonadotropins typically last in the short protocol?
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What role does FSH play in the ovarian stimulation process?
What role does FSH play in the ovarian stimulation process?
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What is the significance of monitoring oestradiol levels during stimulation?
What is the significance of monitoring oestradiol levels during stimulation?
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What surgical procedure is performed to remove eggs from the ovaries?
What surgical procedure is performed to remove eggs from the ovaries?
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What hormone is injected to induce the final maturation of eggs?
What hormone is injected to induce the final maturation of eggs?
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What effect do GnRH antagonists have during the last days of stimulation?
What effect do GnRH antagonists have during the last days of stimulation?
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What is the main goal of the ovary stimulation protocols?
What is the main goal of the ovary stimulation protocols?
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What is in vitro fertilization (IVF)?
What is in vitro fertilization (IVF)?
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What is the purpose of the stimulation step in IVF?
What is the purpose of the stimulation step in IVF?
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List the basic five steps involved in IVF.
List the basic five steps involved in IVF.
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What are fertility drugs and their role in IVF?
What are fertility drugs and their role in IVF?
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On what day of the menstrual cycle is treatment generally started for IVF?
On what day of the menstrual cycle is treatment generally started for IVF?
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What is an embryo in the context of IVF?
What is an embryo in the context of IVF?
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What conditions might make IVF advisable?
What conditions might make IVF advisable?
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Why is the production of multiple eggs important in IVF?
Why is the production of multiple eggs important in IVF?
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Study Notes
In Vitro Fertilization and Embryo Transfer
- IVF is a type of assistive reproductive technology (ART)
- It involves retrieving eggs from a woman's ovaries and fertilizing them with sperm
- The fertilized egg, called an embryo, is then transferred to the woman's uterus
- This procedure can assist with fertility issues or prevent genetic problems
- It's beneficial for tubal disease, endometriosis, unexplained infertility, male factor infertility, and failed induction.
Steps of IVF
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Step 1: Stimulation:
- Also known as super ovulation
- Requires multiple eggs for increased embryo viability
- Fertility drugs (exogenous gonadotropins) stimulate multiple egg production
- Treatment cycles usually start on the third day of menstruation.
- Regular transvaginal ultrasounds and blood tests to monitor hormone levels are necessary
- Two main protocols for stimulation: lengthy (GnRH agonist) and short (injectable gonadotropins)
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Step 2: Egg Retrieval:
- Also known as follicular aspiration
- Minor surgical procedure to remove eggs
- Final maturation is induced by hCG injection, which causes ovulation
- Eggs are retrieved 24-36 hours after hCG injection
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Step 3: Insemination and Fertilization:
- Selection of the best quality embryos
- Sperm washing to remove inactive cells and seminal fluids
- Sperm incubation with the egg for fertilization
- Intracytoplasmic sperm injection (ICSI) may be used in cases of low sperm count or motility
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Step 4: Embryo Culture:
- Embryos are cultured for 3 days after retrieval
- Culture can be performed in artificial culture medium or autologous endometrial co-culture
- Media for artificial culture typically contains glucose, pyruvate, and energy-supplying components
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Step 5: Embryo Selection and Transfer:
- Embryo selection based on cell number, growth, and fragmentation
- High-quality embryos are chosen to maximize pregnancy rates
- Transferred to the woman's uterus using a thin plastic catheter
- Depends on number of embryos available, woman's age, and other factors.
Risks of IVF
- Multiple Births: Increased risk of premature birth and low birth weight
- Premature Birth and Low Body Weight: Babies born from IVF may be born earlier than normal
- Ovarian Hyperstimulation Syndrome (OHSS): Ovaries swell and become painful due to fertility drugs
- Miscarriage: Similar rates to natural pregnancies, especially in older women
- Ectopic Pregnancy: Fertilized egg implants outside the uterus, which is not viable
- Birth Defects: Further research needed to determine if IVF is associated with an increased risk
Stem Cell Technology
- Stem Cells: Primitive, unspecialized cells that can develop into various specialized cell types
- Stem Cell Applications: Heart disease, diabetes, arthritis, brain disease, eye diseases
- Diabetes: Stem cell therapy may be used to normalize glucose and insulin levels in diabetic patients
- Parkinson's Disease: Stem cells can be grown into new brain cells, replacing damaged cells, and used to test new drugs.
Biopesticides
- Biopesticides: Derived from natural materials (plants, bacteria, fungi, viruses)
- Types: Microbial and plant-incorporated protectants (PIPs)
- Microbial Biopesticides: Naturally occurring microorganisms that control pests
- PIPs: Plants genetically modified to produce pest-controlling substances
- Biochemical Biopesticides: Naturally occurring substances that disrupt insect mating or control pests via pheromones.
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Description
This quiz covers the essentials of In Vitro Fertilization (IVF) and the steps involved in the process. Learn about the retrieval of eggs, fertilization, and embryo transfer, as well as the various fertility issues IVF can address. Test your understanding of the significant protocols used in IVF treatment.