Immunology and Blood Types Quiz
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Questions and Answers

Which antibody class is primarily responsible for allergic reactions?

  • IgE (correct)
  • IgD
  • IgG
  • IgA
  • Which of the following is NOT a type of hypersensitivity reaction?

  • Delayed-type
  • Cell-mediated (correct)
  • Cytotoxic
  • Immune Complex
  • Which blood group system is primarily responsible for the development of hemolytic disease of the newborn (HDN)?

  • Kell
  • ABO
  • MNS
  • Rh (correct)
  • What laboratory test is used to detect the presence of antibodies against red blood cells in a patient's serum?

    <p>Indirect Coombs (C)</p> Signup and view all the answers

    Which type of blood product is indicated for patients with severe thrombocytopenia?

    <p>Platelets (D)</p> Signup and view all the answers

    A patient with CD4 count below 200 is diagnosed with:

    <p>AIDS (D)</p> Signup and view all the answers

    Which of the following blood types contains anti-A and anti-B antibodies?

    <p>Type O (D)</p> Signup and view all the answers

    What is the function of the CD4 protein on the surface of immune cells?

    <p>Signaling CD8 to activate cytotoxic T cells (C)</p> Signup and view all the answers

    A patient with O- blood type requires a transfusion. What blood types could be safely transfused?

    <p>O-, A-, B-, AB- (B)</p> Signup and view all the answers

    Which of the following is NOT a typical finding in a complete blood count (CBC) for a patient with HIV?

    <p>Thrombocytosis (B)</p> Signup and view all the answers

    Which of the following statements is TRUE about the Rh factor?

    <p>The Rh factor is a protein found on the surface of red blood cells and its presence or absence is determined by a single gene. (D)</p> Signup and view all the answers

    Which of the following tests is used to confirm the presence of HIV and distinguish its type?

    <p>HIV differentiation immunoassay (C)</p> Signup and view all the answers

    What is the primary purpose of the CD4 test in HIV management?

    <p>To monitor treatment effectiveness (A)</p> Signup and view all the answers

    A patient with AB+ blood type requires a transfusion. Which of the following blood types can be safely transfused?

    <p>All of the above (D)</p> Signup and view all the answers

    Which blood type is considered the universal recipient?

    <p>Type AB (B)</p> Signup and view all the answers

    What is the main reason why donor plasma antibodies are less concerning in a transfusion than recipient plasma antibodies?

    <p>Donor antibodies are usually in lower concentrations and diluted by the recipient's plasma, causing minimal damage to recipient red blood cells. (A)</p> Signup and view all the answers

    What is the purpose of a Coombs test?

    <p>To identify the presence of antibodies against red blood cells in a patient's blood, which can be a sign of a blood transfusion reaction or an autoimmune disease. (C)</p> Signup and view all the answers

    Which blood type is considered the universal donor?

    <p>Type O (C)</p> Signup and view all the answers

    Which blood product is indicated for patients with a deficiency in Factors VIII and XIII, fibrinogen, and von Willebrand factor?

    <p>Cryoprecipitate (C)</p> Signup and view all the answers

    What is the significance of a positive urine nitrite test?

    <p>Indicates a possible bacterial infection (C)</p> Signup and view all the answers

    Which of the following laboratory tests is used to monitor the effectiveness of anticoagulant therapy?

    <p>Prothrombin time (PT)/International Normalized Ratio (INR) (B)</p> Signup and view all the answers

    What is the primary function of red blood cells?

    <p>Carry oxygen to tissues (A)</p> Signup and view all the answers

    Which blood product is typically indicated for patients with severe thrombocytopenia?

    <p>Platelets (A)</p> Signup and view all the answers

    Which immunoglobulin subclass constitutes the largest percentage of serum immunoglobulins?

    <p>IgG (A)</p> Signup and view all the answers

    Which immunoglobulin is primarily responsible for ABO blood grouping?

    <p>IgM (B)</p> Signup and view all the answers

    Which type of hypersensitivity reaction is IgE-mediated and often responsible for common allergies?

    <p>Type I (A)</p> Signup and view all the answers

    What immune function is associated with IgG but NOT with IgE?

    <p>Activation of the complement system. (D)</p> Signup and view all the answers

    Which immunoglobulin is primarily responsible for immune defense against pathogens in mucosal tissues?

    <p>IgA (A)</p> Signup and view all the answers

    Which type of hypersensitivity reaction involves the deposition of antigen-antibody complexes in vessels and joints?

    <p>Type III (A)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of a Type II hypersensitivity reaction?

    <p>Primarily mediated by T-cell overactivation. (C)</p> Signup and view all the answers

    What is the significance of elevated IgM levels in a newborn?

    <p>Indicates infection during gestation. (D)</p> Signup and view all the answers

    Which immunoglobulin is associated with allergic reactions and parasitic infections?

    <p>IgE (B)</p> Signup and view all the answers

    What is the main difference between polyclonal and monoclonal antibodies?

    <p>Monoclonal antibodies target a single epitope of the same antigen. (A)</p> Signup and view all the answers

    Which of the following is an example of a Type IV hypersensitivity reaction?

    <p>Allergic contact dermatitis (C)</p> Signup and view all the answers

    Which immunoglobulin is often used to monitor the course of hypersensitivity reactions, immune deficiencies, and autoimmune diseases?

    <p>IgG (C)</p> Signup and view all the answers

    What is the main difference between autoimmune diseases and immunodeficiency disorders?

    <p>Autoimmune diseases involve an overactive immune system, while immunodeficiency disorders involve an inadequate immune system. (D)</p> Signup and view all the answers

    Which of the following is NOT a common characteristic of immunodeficiency disorders?

    <p>Increased production of inflammatory markers (D)</p> Signup and view all the answers

    What is the main purpose of the Gell and Coombs classification system?

    <p>To categorize the various types of hypersensitivity reactions. (B)</p> Signup and view all the answers

    Which of the following is true about the direct Coombs test (DAT)?

    <p>DAT confirms immune-mediated hemolytic anemia by testing if there are antibodies against the patient's own RBCs. (C)</p> Signup and view all the answers

    What is the purpose of the indirect Coombs test (IAT)?

    <p>To identify if antibodies against RBCs exist in the patient's serum by testing the reaction with RBCs with predetermined antigens. (C)</p> Signup and view all the answers

    What is the primary goal of the 'Type and Screen' procedure?

    <p>To identify the patient's ABO grouping and Rh type and screen for common antibodies. (B)</p> Signup and view all the answers

    What differentiates 'Type and Screen' from 'Type and Cross'?

    <p>'Type and Screen' only determines blood type and screens for common antibodies, while 'Type and Cross' involves an indirect Coombs test to assess compatibility with donor blood (A)</p> Signup and view all the answers

    Which blood product is recommended for patients with very low hemoglobin, chronic anemia, or cardiopulmonary disease?

    <p>Packed Red Blood Cells (C)</p> Signup and view all the answers

    What is the primary indication for administering Platelet Rich Plasma (PRP)?

    <p>Patients with low platelet counts at risk of spontaneous bleeding. (A)</p> Signup and view all the answers

    What is the advantage of using the new method of PRP apheresis as compared to the old method of PRP preparation?

    <p>The new method results in concentrated platelets from a single donor. (D)</p> Signup and view all the answers

    In an emergency situation, when transfusion is urgently needed but 'Type and Cross' cannot be performed due to time constraints, what type of blood might be administered?

    <p>O negative blood (A)</p> Signup and view all the answers

    Flashcards

    Antigens and Antibodies

    Substances that trigger the immune response; antibodies are proteins produced against these antigens.

    Types of Immunoglobulins

    Different classes of antibodies: IgA, IgD, IgE, IgG, IgM, each with distinct roles in immune response.

    Immune Response

    The body's defense reaction involving cell-mediated and humoral immunity to pathogens.

    Hypersensitivity Types

    Four types of abnormal immune reactions: Type I (allergic), Type II (cytotoxic), Type III (immune complex), Type IV (delayed).

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    HIV Transmission and Testing

    HIV is a virus transmitted through bodily fluids; important tests include ELISA and Western blot for diagnosis.

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    Chédiak-Higashi Syndrome

    A rare immunodeficiency disorder that affects white blood cells and leads to increased infections.

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    Chronic Granulomatous Disease (CGD)

    A genetic disorder where white blood cells cannot kill certain bacteria, leading to repeated infections.

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    CD4 T cells

    Immune cells crucial for signaling other immune responses, especially in HIV.

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    HIV Diagnosis Assay

    A test that confirms HIV infection and distinguishes its type using antibody detection.

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    CD4 Count Importance

    Measures the health of the immune system; <200 indicates AIDS, higher shows better response to treatment.

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    ABO Blood Types

    Classification of human blood based on the presence or absence of antigens A and B.

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    Type O Blood

    Blood type that lacks A and B antigens but has both anti-A and anti-B antibodies, making it a universal donor.

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    Blood Type Compatibility

    Rules for transfusions: Type O can donate to anyone, AB can receive from anyone; others have specific pairs.

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    Rh Factor

    Erythrocyte antigen discovered in rhesus monkeys, indicating blood type positivity or negativity.

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    Blood Type Prevalence

    Percentage distribution of blood types in the population, e.g., O+ is most common.

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    Transfusion Reaction

    Incompatible blood exposure leading to clumping and hemolysis of RBCs, potentially fatal.

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    Coombs Test

    Test that checks for antibodies against RBCs, used for conditions like jaundice.

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    Agglutination Reaction

    The clumping of red blood cells due to antigen-antibody interaction during transfusion.

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    Fresh Frozen Plasma (FFP)

    A blood product rich in clotting factors used for patients with bleeding and factor deficiencies.

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    Cryoprecipitate

    A blood product obtained from FFP, containing concentrated factors such as VIII and fibrinogen.

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    Clotting Factor Deficiencies

    Conditions where specific clotting factors are lacking, causing bleeding issues.

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    Platelets in Blood Testing

    Cell fragments in blood crucial for clotting; measured for bleeding disorders.

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    Indications for Blood Products

    Clinical situations that warrant the use of plasma or platelets for treatment.

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    Coombs Reagent

    A reagent used to detect antibodies against RBCs.

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    Direct Coombs Test

    Test that checks if antibodies are attacking a patient’s RBCs.

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    Indirect Coombs Test

    Test for antibodies in serum that react with RBC antigens.

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    Autoimmune Hemolytic Anemia

    Condition where the immune system destroys RBCs.

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    Type and Screen

    Procedure determining blood type (ABO and Rh) and common antibodies.

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    Type and Cross

    Combines type and screen with a compatibility test with donor blood.

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    Packed Red Blood Cells (PRBC)

    Concentrated red blood cells used for transfusion in low Hgb situations.

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    Platelet Rich Plasma (PRP)

    Concentrated platelets from a single donor, used to prevent bleeding.

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    Cytokines

    Signaling proteins released by cells that affect other cells in the immune response.

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    Antigen Presenting Cells (APCs)

    Cells that display antigens to T-cells, initiating an immune response.

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    B-cells

    Immune cells that produce antibodies; include memory B-cells and plasma B-cells.

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    Immunoglobulins

    Another term for antibodies, proteins that help identify and neutralize pathogens.

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    IgG

    The most abundant antibody in serum, crucial for immune defense and can cross the placenta.

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    IgA

    An antibody found in mucosal areas like tears and saliva; protects mucosal surfaces.

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    IgM

    The first antibody produced in response to infection, crucial for blood grouping.

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    IgE

    An antibody associated with allergic reactions and responses to parasites.

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    Hypersensitivity

    An exaggerated immune response that can lead to discomfort or severe reactions.

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    Type I Hypersensitivity

    Immediate allergic reaction mediated by IgE; includes asthma and anaphylaxis.

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    Type II Hypersensitivity

    Antibody-mediated reaction where self-cells are attacked; includes transfusion reactions.

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    Type III Hypersensitivity

    Immune complex-mediated reaction where antibodies bind to antigens leading to inflammation.

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    Type IV Hypersensitivity

    T-cell mediated delayed response leading to tissue damage and inflammation.

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    Autoimmune Diseases

    Conditions where the immune system attacks the body's own tissues; often have high inflammatory markers.

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    Study Notes

    Immunology Week 4

    • This week's focus is immunology, taught by Hanzely, DMSc, PA-C, RD
    • Objectives cover various aspects of the immune response and related diagnostics
    • Inventorying antigens and antibodies (IgA, IgD, IgE, IgG, IgM) is a key objective
    • Understanding cell-mediated and humoral immunity is critical for this week
    • Four types of hypersensitivity will be inspected in detail
    • Autoimmune and immunodeficiency diseases will be compared
    • HIV, its transmission, and diagnostic/monitoring procedures are included
    • This week's content also deals with major blood group systems ABO and Rh, contrasting anti-Rh antibodies and HDN
    • Laboratory techniques like Coombs testing (direct/indirect), Type and Cross Match, Type and Screen, and transfusion indications for red blood cells, cryoprecipitate, platelets, and fresh frozen plasma are covered
    • Possible transfusion reactions are part of the learning materials

    Immune Response

    • Innate immunity features nonspecific barriers (skin, GI lining) and phagocytes (neutrophils, macrophages)
    • Adaptive immunity targets specific antigens, with cell-mediated immunity (T-lymphocytes) and antibody-mediated immunity (B-lymphocytes) being key components.
    • The immune response begins with local neutrophils encountering pathogens and initiating phagocytosis, triggering cytokines that promote vasodilation and recruit more neutrophils and monocytes that mature into macrophages.
    • Macrophages act as antigen-presenting cells (APCs), presenting antigens to T-cells (cytotoxic, helper, regulatory). B-cells are activated, some becoming memory B-cells for faster responses in the future, while other B-cells mature into plasma cells secreting antibodies (immunoglobulins).

    Immunoglobulins

    • Immunoglobulins (Ig's) are used synonymously with antibodies
    • They are measured in mg/dL and reference ranges vary by age and method
    • Ig testing helps monitor hypersensitivity, immune deficiencies, autoimmune diseases, and vaccine responses, but isn't diagnostic.
    • Electrophoresis separates Igs based on mass and charge, revealing five isotypes: IgG, IgA, IgM, IgE, and IgD

    Monoclonal vs Polyclonal Antibodies

    • Polyclonal antibodies bind to different epitopes of the same antigen
    • Monoclonal antibodies bind to a single epitope of an antigen
    • These differences are utilized in drug development, research, and laboratory evaluations

    Immunoglobulin G (IgG)

    • Makes up approximately 75% of serum immunoglobulins
    • Major circulating antibody that crosses the placenta
    • Critical in various bodily functions including neutralization, opsonization, complement activation, and antibody-dependent cytotoxicity
    • Increased presence may indicate various diseases, while decreased levels suggest potential issues including immune deficiencies

    Immunoglobulin A (IgA)

    • IgA accounts for roughly 15% of immunoglobulins
    • Found in bodily fluids like tears, blood, and secretions of the respiratory and GI systems
    • Plays a role in protecting mucosal tissues from pathogens
    • Increased IgA levels may be associated with certain inflammatory conditions, while decreased levels might suggest underlying blood disorders.

    Immunoglobulin M (IgM)

    • Primarily involved in ABO blood grouping and responds quickly to infections
    • The first antibody produced in an immune response, characterized by a pentamer structure with numerous antigen-binding sites
    • Plays a critical role in activating complement and forming complexes
    • Elevated IgM in newborns suggests possible in-utero infections

    Immunoglobulin E (IgE)

    • Is involved in mediating allergic and parasitic responses
    • Important in diagnosing allergic diseases and hypersensitivity, although not useful as a standalone test.
    • It does not activate the complement system, nor is it significant for bacterial infections or opsonization.
    • Elevated levels indicate various diseases such as atopic dermatitis, asthma, and certain chronic conditions

    Immunoglobulin D (IgD)

    • This immunoglobulin is not very well understood
    • Its role appears primarily associated with B-cell function during immune responses but doesn't participate directly.
    • It is seldom assessed.

    Hypersensitivity

    • An exaggerated immune response, ranging from discomfort to fatality
    • Gell and Coombs classification system groups hypersensitivity into four types, with varying clinical significance
    • These types (I, II, III, and IV) are central to understanding immune reactions and their consequences. Type I hypersensitivity is mediated by IgE and characterized by immediate reactions. Type II hypersensitivity involves IgG or IgM and is associated with reactions against self-cells or foreign cells. Type III reactions involve IgG antibodies and antigen complexes. In Type IV hypersensitivity, T-cells mediate the reaction and it can take days to fully manifest
    • Inflammatory reactions, such as allergic contact dermatitis, autoimmune disorders like Type 1 diabetes, and some inflammatory bowel diseases, can occur due to hypersensitivity reactions

    Blood Types

    • Blood type is determined by antigens on the surface of red blood cells
    • ABO blood types (A, B, AB, O) and the Rh factor are critical in blood transfusions
    • Compatibility is essential during blood transfusions to avoid immune reactions such as hemolysis
    • Understanding these systems and procedures like type and screen, and cross-matching blood components is vital for preventing complications, especially in cases of HDN

    Coombs Tests

    • Used to check for antibodies in the blood that may react with red blood cells.
    • Direct Coombs tests detect antibodies directly bound to red blood cells, often used in the diagnosis of autoimmune hemolytic anemia, transfusion reactions and HDN
    • Indirect Coombs tests detect antibodies in the blood serum that may bind to red blood cells
    • Crucial in evaluating compatibility for transfusions and in pregnancies when there may be a risk of HDN

    Blood Product Indications

    • Blood components like whole blood, packed red cells, platelet-rich plasma, fresh frozen plasma, and cryoprecipitate have specific indications
    • Each component serves different purposes in patient care, such as treating anemia, supporting clotting function, or replacing blood loss

    HIV

    • HIV is a virus targeting the immune system, specifically CD4+ T cells important in cell signaling, that causes immunodeficiency
    • Its diagnosis involves the HIV differentiation immunoassay or serology test to confirm and determine the type of HIV present, as well as HIV RNA quantification for monitoring after a diagnosis
    • CD4 tests play in measuring treatment progress, risk of infection and response to HIV treatment

    Autoimmune vs. Immunodeficiency

    • Autoimmunity involves the immune system attacking its own healthy tissues
    • Immunodeficiency describes a weakened immune system, leading to increased susceptibility to infections

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    Description

    Test your knowledge on immunology and blood types with this quiz. Explore topics such as antibody classes, hypersensitivity reactions, and blood transfusion compatibility. Perfect for students in medical or health-related fields.

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