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Questions and Answers
Which antibody class is primarily responsible for allergic reactions?
Which antibody class is primarily responsible for allergic reactions?
Which of the following is NOT a type of hypersensitivity reaction?
Which of the following is NOT a type of hypersensitivity reaction?
Which blood group system is primarily responsible for the development of hemolytic disease of the newborn (HDN)?
Which blood group system is primarily responsible for the development of hemolytic disease of the newborn (HDN)?
What laboratory test is used to detect the presence of antibodies against red blood cells in a patient's serum?
What laboratory test is used to detect the presence of antibodies against red blood cells in a patient's serum?
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Which type of blood product is indicated for patients with severe thrombocytopenia?
Which type of blood product is indicated for patients with severe thrombocytopenia?
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A patient with CD4 count below 200 is diagnosed with:
A patient with CD4 count below 200 is diagnosed with:
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Which of the following blood types contains anti-A and anti-B antibodies?
Which of the following blood types contains anti-A and anti-B antibodies?
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What is the function of the CD4 protein on the surface of immune cells?
What is the function of the CD4 protein on the surface of immune cells?
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A patient with O- blood type requires a transfusion. What blood types could be safely transfused?
A patient with O- blood type requires a transfusion. What blood types could be safely transfused?
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Which of the following is NOT a typical finding in a complete blood count (CBC) for a patient with HIV?
Which of the following is NOT a typical finding in a complete blood count (CBC) for a patient with HIV?
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Which of the following statements is TRUE about the Rh factor?
Which of the following statements is TRUE about the Rh factor?
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Which of the following tests is used to confirm the presence of HIV and distinguish its type?
Which of the following tests is used to confirm the presence of HIV and distinguish its type?
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What is the primary purpose of the CD4 test in HIV management?
What is the primary purpose of the CD4 test in HIV management?
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A patient with AB+ blood type requires a transfusion. Which of the following blood types can be safely transfused?
A patient with AB+ blood type requires a transfusion. Which of the following blood types can be safely transfused?
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Which blood type is considered the universal recipient?
Which blood type is considered the universal recipient?
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What is the main reason why donor plasma antibodies are less concerning in a transfusion than recipient plasma antibodies?
What is the main reason why donor plasma antibodies are less concerning in a transfusion than recipient plasma antibodies?
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What is the purpose of a Coombs test?
What is the purpose of a Coombs test?
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Which blood type is considered the universal donor?
Which blood type is considered the universal donor?
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Which blood product is indicated for patients with a deficiency in Factors VIII and XIII, fibrinogen, and von Willebrand factor?
Which blood product is indicated for patients with a deficiency in Factors VIII and XIII, fibrinogen, and von Willebrand factor?
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What is the significance of a positive urine nitrite test?
What is the significance of a positive urine nitrite test?
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Which of the following laboratory tests is used to monitor the effectiveness of anticoagulant therapy?
Which of the following laboratory tests is used to monitor the effectiveness of anticoagulant therapy?
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What is the primary function of red blood cells?
What is the primary function of red blood cells?
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Which blood product is typically indicated for patients with severe thrombocytopenia?
Which blood product is typically indicated for patients with severe thrombocytopenia?
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Which immunoglobulin subclass constitutes the largest percentage of serum immunoglobulins?
Which immunoglobulin subclass constitutes the largest percentage of serum immunoglobulins?
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Which immunoglobulin is primarily responsible for ABO blood grouping?
Which immunoglobulin is primarily responsible for ABO blood grouping?
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Which type of hypersensitivity reaction is IgE-mediated and often responsible for common allergies?
Which type of hypersensitivity reaction is IgE-mediated and often responsible for common allergies?
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What immune function is associated with IgG but NOT with IgE?
What immune function is associated with IgG but NOT with IgE?
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Which immunoglobulin is primarily responsible for immune defense against pathogens in mucosal tissues?
Which immunoglobulin is primarily responsible for immune defense against pathogens in mucosal tissues?
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Which type of hypersensitivity reaction involves the deposition of antigen-antibody complexes in vessels and joints?
Which type of hypersensitivity reaction involves the deposition of antigen-antibody complexes in vessels and joints?
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Which of the following is NOT a characteristic of a Type II hypersensitivity reaction?
Which of the following is NOT a characteristic of a Type II hypersensitivity reaction?
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What is the significance of elevated IgM levels in a newborn?
What is the significance of elevated IgM levels in a newborn?
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Which immunoglobulin is associated with allergic reactions and parasitic infections?
Which immunoglobulin is associated with allergic reactions and parasitic infections?
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What is the main difference between polyclonal and monoclonal antibodies?
What is the main difference between polyclonal and monoclonal antibodies?
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Which of the following is an example of a Type IV hypersensitivity reaction?
Which of the following is an example of a Type IV hypersensitivity reaction?
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Which immunoglobulin is often used to monitor the course of hypersensitivity reactions, immune deficiencies, and autoimmune diseases?
Which immunoglobulin is often used to monitor the course of hypersensitivity reactions, immune deficiencies, and autoimmune diseases?
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What is the main difference between autoimmune diseases and immunodeficiency disorders?
What is the main difference between autoimmune diseases and immunodeficiency disorders?
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Which of the following is NOT a common characteristic of immunodeficiency disorders?
Which of the following is NOT a common characteristic of immunodeficiency disorders?
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What is the main purpose of the Gell and Coombs classification system?
What is the main purpose of the Gell and Coombs classification system?
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Which of the following is true about the direct Coombs test (DAT)?
Which of the following is true about the direct Coombs test (DAT)?
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What is the purpose of the indirect Coombs test (IAT)?
What is the purpose of the indirect Coombs test (IAT)?
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What is the primary goal of the 'Type and Screen' procedure?
What is the primary goal of the 'Type and Screen' procedure?
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What differentiates 'Type and Screen' from 'Type and Cross'?
What differentiates 'Type and Screen' from 'Type and Cross'?
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Which blood product is recommended for patients with very low hemoglobin, chronic anemia, or cardiopulmonary disease?
Which blood product is recommended for patients with very low hemoglobin, chronic anemia, or cardiopulmonary disease?
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What is the primary indication for administering Platelet Rich Plasma (PRP)?
What is the primary indication for administering Platelet Rich Plasma (PRP)?
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What is the advantage of using the new method of PRP apheresis as compared to the old method of PRP preparation?
What is the advantage of using the new method of PRP apheresis as compared to the old method of PRP preparation?
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In an emergency situation, when transfusion is urgently needed but 'Type and Cross' cannot be performed due to time constraints, what type of blood might be administered?
In an emergency situation, when transfusion is urgently needed but 'Type and Cross' cannot be performed due to time constraints, what type of blood might be administered?
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Flashcards
Antigens and Antibodies
Antigens and Antibodies
Substances that trigger the immune response; antibodies are proteins produced against these antigens.
Types of Immunoglobulins
Types of Immunoglobulins
Different classes of antibodies: IgA, IgD, IgE, IgG, IgM, each with distinct roles in immune response.
Immune Response
Immune Response
The body's defense reaction involving cell-mediated and humoral immunity to pathogens.
Hypersensitivity Types
Hypersensitivity Types
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HIV Transmission and Testing
HIV Transmission and Testing
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Chédiak-Higashi Syndrome
Chédiak-Higashi Syndrome
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Chronic Granulomatous Disease (CGD)
Chronic Granulomatous Disease (CGD)
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CD4 T cells
CD4 T cells
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HIV Diagnosis Assay
HIV Diagnosis Assay
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CD4 Count Importance
CD4 Count Importance
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ABO Blood Types
ABO Blood Types
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Type O Blood
Type O Blood
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Blood Type Compatibility
Blood Type Compatibility
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Rh Factor
Rh Factor
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Blood Type Prevalence
Blood Type Prevalence
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Transfusion Reaction
Transfusion Reaction
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Coombs Test
Coombs Test
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Agglutination Reaction
Agglutination Reaction
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Fresh Frozen Plasma (FFP)
Fresh Frozen Plasma (FFP)
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Cryoprecipitate
Cryoprecipitate
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Clotting Factor Deficiencies
Clotting Factor Deficiencies
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Platelets in Blood Testing
Platelets in Blood Testing
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Indications for Blood Products
Indications for Blood Products
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Coombs Reagent
Coombs Reagent
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Direct Coombs Test
Direct Coombs Test
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Indirect Coombs Test
Indirect Coombs Test
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Autoimmune Hemolytic Anemia
Autoimmune Hemolytic Anemia
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Type and Screen
Type and Screen
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Type and Cross
Type and Cross
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Packed Red Blood Cells (PRBC)
Packed Red Blood Cells (PRBC)
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Platelet Rich Plasma (PRP)
Platelet Rich Plasma (PRP)
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Cytokines
Cytokines
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Antigen Presenting Cells (APCs)
Antigen Presenting Cells (APCs)
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B-cells
B-cells
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Immunoglobulins
Immunoglobulins
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IgG
IgG
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IgA
IgA
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IgM
IgM
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IgE
IgE
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Hypersensitivity
Hypersensitivity
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Type I Hypersensitivity
Type I Hypersensitivity
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Type II Hypersensitivity
Type II Hypersensitivity
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Type III Hypersensitivity
Type III Hypersensitivity
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Type IV Hypersensitivity
Type IV Hypersensitivity
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Autoimmune Diseases
Autoimmune Diseases
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Study Notes
Immunology Week 4
- This week's focus is immunology, taught by Hanzely, DMSc, PA-C, RD
- Objectives cover various aspects of the immune response and related diagnostics
- Inventorying antigens and antibodies (IgA, IgD, IgE, IgG, IgM) is a key objective
- Understanding cell-mediated and humoral immunity is critical for this week
- Four types of hypersensitivity will be inspected in detail
- Autoimmune and immunodeficiency diseases will be compared
- HIV, its transmission, and diagnostic/monitoring procedures are included
- This week's content also deals with major blood group systems ABO and Rh, contrasting anti-Rh antibodies and HDN
- Laboratory techniques like Coombs testing (direct/indirect), Type and Cross Match, Type and Screen, and transfusion indications for red blood cells, cryoprecipitate, platelets, and fresh frozen plasma are covered
- Possible transfusion reactions are part of the learning materials
Immune Response
- Innate immunity features nonspecific barriers (skin, GI lining) and phagocytes (neutrophils, macrophages)
- Adaptive immunity targets specific antigens, with cell-mediated immunity (T-lymphocytes) and antibody-mediated immunity (B-lymphocytes) being key components.
- The immune response begins with local neutrophils encountering pathogens and initiating phagocytosis, triggering cytokines that promote vasodilation and recruit more neutrophils and monocytes that mature into macrophages.
- Macrophages act as antigen-presenting cells (APCs), presenting antigens to T-cells (cytotoxic, helper, regulatory). B-cells are activated, some becoming memory B-cells for faster responses in the future, while other B-cells mature into plasma cells secreting antibodies (immunoglobulins).
Immunoglobulins
- Immunoglobulins (Ig's) are used synonymously with antibodies
- They are measured in mg/dL and reference ranges vary by age and method
- Ig testing helps monitor hypersensitivity, immune deficiencies, autoimmune diseases, and vaccine responses, but isn't diagnostic.
- Electrophoresis separates Igs based on mass and charge, revealing five isotypes: IgG, IgA, IgM, IgE, and IgD
Monoclonal vs Polyclonal Antibodies
- Polyclonal antibodies bind to different epitopes of the same antigen
- Monoclonal antibodies bind to a single epitope of an antigen
- These differences are utilized in drug development, research, and laboratory evaluations
Immunoglobulin G (IgG)
- Makes up approximately 75% of serum immunoglobulins
- Major circulating antibody that crosses the placenta
- Critical in various bodily functions including neutralization, opsonization, complement activation, and antibody-dependent cytotoxicity
- Increased presence may indicate various diseases, while decreased levels suggest potential issues including immune deficiencies
Immunoglobulin A (IgA)
- IgA accounts for roughly 15% of immunoglobulins
- Found in bodily fluids like tears, blood, and secretions of the respiratory and GI systems
- Plays a role in protecting mucosal tissues from pathogens
- Increased IgA levels may be associated with certain inflammatory conditions, while decreased levels might suggest underlying blood disorders.
Immunoglobulin M (IgM)
- Primarily involved in ABO blood grouping and responds quickly to infections
- The first antibody produced in an immune response, characterized by a pentamer structure with numerous antigen-binding sites
- Plays a critical role in activating complement and forming complexes
- Elevated IgM in newborns suggests possible in-utero infections
Immunoglobulin E (IgE)
- Is involved in mediating allergic and parasitic responses
- Important in diagnosing allergic diseases and hypersensitivity, although not useful as a standalone test.
- It does not activate the complement system, nor is it significant for bacterial infections or opsonization.
- Elevated levels indicate various diseases such as atopic dermatitis, asthma, and certain chronic conditions
Immunoglobulin D (IgD)
- This immunoglobulin is not very well understood
- Its role appears primarily associated with B-cell function during immune responses but doesn't participate directly.
- It is seldom assessed.
Hypersensitivity
- An exaggerated immune response, ranging from discomfort to fatality
- Gell and Coombs classification system groups hypersensitivity into four types, with varying clinical significance
- These types (I, II, III, and IV) are central to understanding immune reactions and their consequences. Type I hypersensitivity is mediated by IgE and characterized by immediate reactions. Type II hypersensitivity involves IgG or IgM and is associated with reactions against self-cells or foreign cells. Type III reactions involve IgG antibodies and antigen complexes. In Type IV hypersensitivity, T-cells mediate the reaction and it can take days to fully manifest
- Inflammatory reactions, such as allergic contact dermatitis, autoimmune disorders like Type 1 diabetes, and some inflammatory bowel diseases, can occur due to hypersensitivity reactions
Blood Types
- Blood type is determined by antigens on the surface of red blood cells
- ABO blood types (A, B, AB, O) and the Rh factor are critical in blood transfusions
- Compatibility is essential during blood transfusions to avoid immune reactions such as hemolysis
- Understanding these systems and procedures like type and screen, and cross-matching blood components is vital for preventing complications, especially in cases of HDN
Coombs Tests
- Used to check for antibodies in the blood that may react with red blood cells.
- Direct Coombs tests detect antibodies directly bound to red blood cells, often used in the diagnosis of autoimmune hemolytic anemia, transfusion reactions and HDN
- Indirect Coombs tests detect antibodies in the blood serum that may bind to red blood cells
- Crucial in evaluating compatibility for transfusions and in pregnancies when there may be a risk of HDN
Blood Product Indications
- Blood components like whole blood, packed red cells, platelet-rich plasma, fresh frozen plasma, and cryoprecipitate have specific indications
- Each component serves different purposes in patient care, such as treating anemia, supporting clotting function, or replacing blood loss
HIV
- HIV is a virus targeting the immune system, specifically CD4+ T cells important in cell signaling, that causes immunodeficiency
- Its diagnosis involves the HIV differentiation immunoassay or serology test to confirm and determine the type of HIV present, as well as HIV RNA quantification for monitoring after a diagnosis
- CD4 tests play in measuring treatment progress, risk of infection and response to HIV treatment
Autoimmune vs. Immunodeficiency
- Autoimmunity involves the immune system attacking its own healthy tissues
- Immunodeficiency describes a weakened immune system, leading to increased susceptibility to infections
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Description
Test your knowledge on immunology and blood types with this quiz. Explore topics such as antibody classes, hypersensitivity reactions, and blood transfusion compatibility. Perfect for students in medical or health-related fields.