12 Questions
What is the purpose of a conjugated secondary antibody in a sandwich ELISA?
To amplify the signal produced by the antigen-antibody complex
What is the role of capture antibody in a sandwich ELISA?
To coat the microplate and immobilize the protein of interest
What is the purpose of Western Blot in diagnostic applications?
To identify specific proteins with specific antibodies
What is the membrane made of in a Western Blot technique?
Nitrocellulose or PVDF
What makes Sandwich ELISAs highly specific?
Two antibodies are required to bind to the protein of interest
What drives a colorimetric reaction in Western Blot?
Specific enzyme exposed to an appropriate substrate
What is the main function of the conjugated-detection antibody in a Sandwich ELISA?
To bind to an additional epitope on the target protein
What is the diagnostic application of Western Blot?
Confirmatory HIV test
What is the main purpose of the enzyme-linked immunosorbent assay (ELISA) method?
To analyze the presence of antigen or antibodies in different solutions
What is the difference between direct and indirect ELISA?
Direct ELISA uses a conjugated detection antibody, while indirect ELISA uses an unconjugated primary detection antibody
What is the function of the labeled reactant in the enzyme-linked immunosorbent assay (ELISA) method?
To produce a signal proportional to the amount of analyte in the sample
What is the purpose of the secondary antibody in the enzyme-linked immunosorbent assay (ELISA) method?
To amplify the detection of the antigen
Test your knowledge of diagnostic applications of immunoglobulins with this quiz. Explore the methods and techniques used for analyzing antigen-antibody interactions, including precipitation and enzyme-linked immunosorbent assay (ELISA).
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