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Immunoglobulin Diagnostic Applications Quiz

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Immunoglobulin Diagnostic Applications Quiz

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Questions and Answers

What is the purpose of a conjugated secondary antibody in a sandwich ELISA?

To amplify the signal produced by the antigen-antibody complex (correct)
To immobilize the protein of interest on the microplate
To bind to an additional epitope on the target protein
To coat the microplate for antigen binding

What is the role of capture antibody in a sandwich ELISA?

To detect the specific protein of interest
To coat the microplate and immobilize the protein of interest (correct)
To amplify the signal produced by the antigen-antibody complex
To bind to an additional epitope on the target protein

What is the purpose of Western Blot in diagnostic applications?

To produce a signal proportional to the amount of antigen bound
To induce proteins in the gel to move to the membrane
To separate proteins according to their size by gel electrophoresis
To identify specific proteins with specific antibodies (correct)

What is the membrane made of in a Western Blot technique?

Nitrocellulose or PVDF (B) Signup and view all the answers

What makes Sandwich ELISAs highly specific?

Two antibodies are required to bind to the protein of interest (A) Signup and view all the answers

What drives a colorimetric reaction in Western Blot?

Specific enzyme exposed to an appropriate substrate (C) Signup and view all the answers

What is the main function of the conjugated-detection antibody in a Sandwich ELISA?

To bind to an additional epitope on the target protein (C) Signup and view all the answers

What is the diagnostic application of Western Blot?

Confirmatory HIV test (A) Signup and view all the answers

What is the main purpose of the enzyme-linked immunosorbent assay (ELISA) method?

To analyze the presence of antigen or antibodies in different solutions (A) Signup and view all the answers

What is the difference between direct and indirect ELISA?

Direct ELISA uses a conjugated detection antibody, while indirect ELISA uses an unconjugated primary detection antibody (C) Signup and view all the answers

What is the function of the labeled reactant in the enzyme-linked immunosorbent assay (ELISA) method?

To produce a signal proportional to the amount of analyte in the sample (C) Signup and view all the answers

What is the purpose of the secondary antibody in the enzyme-linked immunosorbent assay (ELISA) method?

To amplify the detection of the antigen (A) Signup and view all the answers