Immunoglobulin Diagnostic Applications Quiz
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Questions and Answers

What is the purpose of a conjugated secondary antibody in a sandwich ELISA?

  • To amplify the signal produced by the antigen-antibody complex (correct)
  • To immobilize the protein of interest on the microplate
  • To bind to an additional epitope on the target protein
  • To coat the microplate for antigen binding
  • What is the role of capture antibody in a sandwich ELISA?

  • To detect the specific protein of interest
  • To coat the microplate and immobilize the protein of interest (correct)
  • To amplify the signal produced by the antigen-antibody complex
  • To bind to an additional epitope on the target protein
  • What is the purpose of Western Blot in diagnostic applications?

  • To produce a signal proportional to the amount of antigen bound
  • To induce proteins in the gel to move to the membrane
  • To separate proteins according to their size by gel electrophoresis
  • To identify specific proteins with specific antibodies (correct)
  • What is the membrane made of in a Western Blot technique?

    <p>Nitrocellulose or PVDF</p> Signup and view all the answers

    What makes Sandwich ELISAs highly specific?

    <p>Two antibodies are required to bind to the protein of interest</p> Signup and view all the answers

    What drives a colorimetric reaction in Western Blot?

    <p>Specific enzyme exposed to an appropriate substrate</p> Signup and view all the answers

    What is the main function of the conjugated-detection antibody in a Sandwich ELISA?

    <p>To bind to an additional epitope on the target protein</p> Signup and view all the answers

    What is the diagnostic application of Western Blot?

    <p>Confirmatory HIV test</p> Signup and view all the answers

    What is the main purpose of the enzyme-linked immunosorbent assay (ELISA) method?

    <p>To analyze the presence of antigen or antibodies in different solutions</p> Signup and view all the answers

    What is the difference between direct and indirect ELISA?

    <p>Direct ELISA uses a conjugated detection antibody, while indirect ELISA uses an unconjugated primary detection antibody</p> Signup and view all the answers

    What is the function of the labeled reactant in the enzyme-linked immunosorbent assay (ELISA) method?

    <p>To produce a signal proportional to the amount of analyte in the sample</p> Signup and view all the answers

    What is the purpose of the secondary antibody in the enzyme-linked immunosorbent assay (ELISA) method?

    <p>To amplify the detection of the antigen</p> Signup and view all the answers

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