Podcast
Play an AI-generated podcast conversation about this lesson
Questions and Answers
What is the purpose of ICC Immunocytochemistry?
What is the purpose of ICC Immunocytochemistry?
- Anatomically localize presence of a specific protein in cells (correct)
- Determine DNA sequence in cells
- Measure cell viability
- Detect cell size
What allows visualization of the protein under a fluorescence microscope in ICC?
What allows visualization of the protein under a fluorescence microscope in ICC?
- Enzyme-labeled substrate
- Primary antibody without any conjugation
- Secondary antibody with a conjugated fluorophore (correct)
- Tertiary antibody with a specific tag
What does ICC allow researchers to determine when an immunopositive signal is found?
What does ICC allow researchers to determine when an immunopositive signal is found?
- Which sub-cellular compartments are expressing the antigen (correct)
- The cell's electrical charge
- The DNA sequence of the cells
- The cell's metabolic rate
What binds to the bound primary antibody in ICC?
What binds to the bound primary antibody in ICC?
Signup and view all the answers
What does IHC Immunohistochemistry combine?
What does IHC Immunohistochemistry combine?
Signup and view all the answers
Flashcards are hidden until you start studying
Study Notes
Immunocytochemistry (ICC)
- The purpose of ICC is to visualize and localize specific proteins or antigens in cells or tissues.
Principle of ICC
- Fluorescence microscopy is used to visualize the protein, enabled by a fluorophore-conjugated secondary antibody that binds to the primary antibody.
Significance of ICC
- When an immunopositive signal is found, ICC allows researchers to determine the presence, location, and quantity of a specific protein or antigen in cells or tissues.
ICC Procedure
- A secondary antibody, conjugated to a fluorophore or enzyme, binds to the bound primary antibody, facilitating detection and visualization.
Immunohistochemistry (IHC)
- IHC combines the principles of immunology and histochemistry to identify specific cell types or antigens in tissue sections.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
