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Immunocytochemistry (ICC) Technique

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@ExultantPeninsula

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Questions and Answers

What is the purpose of ICC Immunocytochemistry?

Anatomically localize presence of a specific protein in cells (correct)

Determine DNA sequence in cells

Measure cell viability

Detect cell size

What allows visualization of the protein under a fluorescence microscope in ICC?

Enzyme-labeled substrate

Primary antibody without any conjugation

Secondary antibody with a conjugated fluorophore (correct)

Tertiary antibody with a specific tag

What does ICC allow researchers to determine when an immunopositive signal is found?

Which sub-cellular compartments are expressing the antigen (correct)

The cell's electrical charge

The DNA sequence of the cells

The cell's metabolic rate

What binds to the bound primary antibody in ICC?

<p>The secondary antibody (conjugated or labeled)</p> Signup and view all the answers

What does IHC Immunohistochemistry combine?

<p>Anatomy, physiology, immunology, and biochemistry</p> Signup and view all the answers

Study Notes

Immunocytochemistry (ICC)

The purpose of ICC is to visualize and localize specific proteins or antigens in cells or tissues.

Principle of ICC

Fluorescence microscopy is used to visualize the protein, enabled by a fluorophore-conjugated secondary antibody that binds to the primary antibody.

Significance of ICC

When an immunopositive signal is found, ICC allows researchers to determine the presence, location, and quantity of a specific protein or antigen in cells or tissues.

ICC Procedure

A secondary antibody, conjugated to a fluorophore or enzyme, binds to the bound primary antibody, facilitating detection and visualization.

Immunohistochemistry (IHC)

IHC combines the principles of immunology and histochemistry to identify specific cell types or antigens in tissue sections.

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Description

Test your knowledge on the Immunocytochemistry (ICC) technique used to localize specific proteins or antigens in cells. This quiz covers the primary and secondary antibodies, visualization methods, and the practical application of ICC in research.