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Questions and Answers
What is the purpose of ICC Immunocytochemistry?
What allows visualization of the protein under a fluorescence microscope in ICC?
What does ICC allow researchers to determine when an immunopositive signal is found?
What binds to the bound primary antibody in ICC?
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What does IHC Immunohistochemistry combine?
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Study Notes
Immunocytochemistry (ICC)
- The purpose of ICC is to visualize and localize specific proteins or antigens in cells or tissues.
Principle of ICC
- Fluorescence microscopy is used to visualize the protein, enabled by a fluorophore-conjugated secondary antibody that binds to the primary antibody.
Significance of ICC
- When an immunopositive signal is found, ICC allows researchers to determine the presence, location, and quantity of a specific protein or antigen in cells or tissues.
ICC Procedure
- A secondary antibody, conjugated to a fluorophore or enzyme, binds to the bound primary antibody, facilitating detection and visualization.
Immunohistochemistry (IHC)
- IHC combines the principles of immunology and histochemistry to identify specific cell types or antigens in tissue sections.
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Description
Test your knowledge on the Immunocytochemistry (ICC) technique used to localize specific proteins or antigens in cells. This quiz covers the primary and secondary antibodies, visualization methods, and the practical application of ICC in research.
