Podcast
Questions and Answers
What is the result of crosslinking in precipitation immunoassays?
What is the result of crosslinking in precipitation immunoassays?
- Formation of a precipitate (correct)
- Increased solubility of the antigen
- A change in turbidity of the solution
- Breakdown of the antibody-antigen complex
What is the minimum valency required for both the antibody and antigen in these techniques?
What is the minimum valency required for both the antibody and antigen in these techniques?
- Univalent
- Bivalent (correct)
- Tetravalent
- Trivalent
What is measured in agglutination immunoassays?
What is measured in agglutination immunoassays?
- The amount of precipitate formed
- The molecular weight of the antigen
- The concentration of the antibody
- The change in turbidity of the solution (correct)
What is the term for the measurement of turbidity in agglutination immunoassays?
What is the term for the measurement of turbidity in agglutination immunoassays?
What is the ratio of antibody to antigen required for precipitation and agglutination to occur?
What is the ratio of antibody to antigen required for precipitation and agglutination to occur?
What is the primary requirement for precipitation and agglutination to occur in immunoassays?
What is the primary requirement for precipitation and agglutination to occur in immunoassays?
Which of the following is a characteristic of agglutination immunoassays?
Which of the following is a characteristic of agglutination immunoassays?
What is the primary difference between precipitation and agglutination immunoassays?
What is the primary difference between precipitation and agglutination immunoassays?
Which of the following is a necessary condition for both precipitation and agglutination to occur?
Which of the following is a necessary condition for both precipitation and agglutination to occur?
What is the underlying physical property being exploited in precipitation and agglutination immunoassays?
What is the underlying physical property being exploited in precipitation and agglutination immunoassays?
Study Notes
Immunoassays: Exploiting Physical Changes in Solution
- Immunoassays exploit two physical properties: precipitation and agglutination, which require a specific ratio of antibody to antigen.
Precipitation
- Occurs when the correct ratio of antibody to antigen is achieved, leading to crosslinking and precipitation (ppt).
- Requires bivalency of both antibody and antigen, meaning the antibody must bind to two antigens and the antigen must bind to two antibodies.
Agglutination
- Similar to precipitation, but crosslinking does not result in precipitation, instead causing a change in turbidity of the solution.
- Change in turbidity can be measured using nephelometry, shining light through the solution.
- Also requires bivalency of both antibody and antigen.
Immunoassays: Exploiting Physical Changes in Solution
- Immunoassays exploit two physical properties: precipitation and agglutination, which require a specific ratio of antibody to antigen.
Precipitation
- Occurs when the correct ratio of antibody to antigen is achieved, leading to crosslinking and precipitation (ppt).
- Requires bivalency of both antibody and antigen, meaning the antibody must bind to two antigens and the antigen must bind to two antibodies.
Agglutination
- Similar to precipitation, but crosslinking does not result in precipitation, instead causing a change in turbidity of the solution.
- Change in turbidity can be measured using nephelometry, shining light through the solution.
- Also requires bivalency of both antibody and antigen.
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Description
Learn about immunoassays that exploit physical changes in solution, including precipitation and agglutination methods. Understand how these techniques work and their requirements.
