Immunoassay Basics

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Questions and Answers

What is the principle behind an immunoassay?

The destruction of an antibody by an antigen
The nonspecific binding of an antibody to an antigen
The enhancement of an antibody by an antigen
The specific binding affinity of an antibody for a specific antigen (correct)

What are the two major requirements for an immunoassay?

An antigen and a laboratory technician
An antibody and a detection system (correct)
An antigen and a detection system
A detection system and a laboratory technician

What is the purpose of an immunoassay?

To quantify the amount of an antibody or antigen present (correct)
To neutralize the activity of an antigen
To produce more antibodies
To identify the structure of an antigen

What is the role of the antibody in an immunoassay?

<p>To bind specifically to an antigen (A)</p> Signup and view all the answers

What is the outcome of an immunoassay?

<p>The quantification of an antibody or antigen (B)</p> Signup and view all the answers

Study Notes

Immunoassay Principles

Immunoassays rely on the specific binding affinity between an antibody and a specific antigen to quantify either the antibody or antigen present.
Two major requirements are necessary for an immunoassay:

Requirements for Immunoassay

An antibody, which is essential for binding with the antigen.
A detection system, which is necessary for measuring the binding reaction.

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Description

Learn about the principles of immunoassay, a laboratory technique that utilizes the specific binding affinity between an antibody and an antigen to quantify their presence. Discover the two major requirements for an immunoassay.