Immunology: Immunoassay Principles
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Questions and Answers

What does the term 'immunoassay' literally mean?

  • Immune system based measurement
  • Measuring the immune system and an assay
  • Measuring the immune response
  • Immune system assay (correct)
  • What is the primary mechanism used in an immunoassay to quantify the target molecule?

  • Specific binding affinity between an enzyme and substrate
  • Specific binding affinity between a hormone and receptor
  • Specific binding affinity between a protein and carbohydrate
  • Specific binding affinity between an antibody and antigen (correct)
  • What are the two major requirements for an immunoassay?

  • An antibody and a detection system (correct)
  • A hormone and a receptor
  • An enzyme and a protein
  • An antigen and a detection system
  • What is the purpose of an immunoassay?

    <p>To quantify the amount of antigen or antibody present</p> Signup and view all the answers

    What is the function of an antibody in an immunoassay?

    <p>To bind specifically to a specific antigen</p> Signup and view all the answers

    What enables immunoassays to distinguish between different antigens?

    <p>The affinity of an antibody for a specific antigen</p> Signup and view all the answers

    What is the role of an antibody in an immunoassay?

    <p>To bind specifically to a specific antigen</p> Signup and view all the answers

    What is being measured in an immunoassay?

    <p>The amount of antigen present</p> Signup and view all the answers

    Why are antibodies used in immunoassays?

    <p>Because they are specific to certain antigens</p> Signup and view all the answers

    What is a necessary component of an immunoassay, in addition to an antibody?

    <p>A detection system</p> Signup and view all the answers

    Study Notes

    Immunoassay

    • Immunoassay is a diagnostic technique that combines the principles of the immune system and assay.
    • The term "immuno" refers to the immune system, which involves components like antibodies and antigens.
    • The term "assay" means to determine the quantity of a substance present.
    • Immunoassay utilizes the specific binding affinity of an antibody for a specific antigen to quantify the amount of either the antibody or antigen present.

    Requirements for Immunoassay

    • Two major requirements for an immunoassay are:
    • An antibody
    • A detection system

    Immunoassays

    • Immunoassays exploit the specific affinity between antibodies and antigens to determine the quantity of either the antibody or antigen present.
    • The term "immunoassay" is derived from "immuno" (relating to the immune system, involving antibodies and antigens) and "assay" (meaning to determine how much of something is present).
    • Two major requirements for an immunoassay are:
      • An antibody
      • A detection system

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    Description

    Learn about the principles of immunoassay, a laboratory technique used to measure the concentration of antibodies or antigens in a sample. Understand the specific binding affinity between antibodies and antigens and the two major requirements for an immunoassay.

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