10 Questions
What does the term 'immunoassay' literally mean?
Immune system assay
What is the primary mechanism used in an immunoassay to quantify the target molecule?
Specific binding affinity between an antibody and antigen
What are the two major requirements for an immunoassay?
An antibody and a detection system
What is the purpose of an immunoassay?
To quantify the amount of antigen or antibody present
What is the function of an antibody in an immunoassay?
To bind specifically to a specific antigen
What enables immunoassays to distinguish between different antigens?
The affinity of an antibody for a specific antigen
What is the role of an antibody in an immunoassay?
To bind specifically to a specific antigen
What is being measured in an immunoassay?
The amount of antigen present
Why are antibodies used in immunoassays?
Because they are specific to certain antigens
What is a necessary component of an immunoassay, in addition to an antibody?
A detection system
Study Notes
Immunoassay
- Immunoassay is a diagnostic technique that combines the principles of the immune system and assay.
- The term "immuno" refers to the immune system, which involves components like antibodies and antigens.
- The term "assay" means to determine the quantity of a substance present.
- Immunoassay utilizes the specific binding affinity of an antibody for a specific antigen to quantify the amount of either the antibody or antigen present.
Requirements for Immunoassay
- Two major requirements for an immunoassay are:
- An antibody
- A detection system
Immunoassays
- Immunoassays exploit the specific affinity between antibodies and antigens to determine the quantity of either the antibody or antigen present.
- The term "immunoassay" is derived from "immuno" (relating to the immune system, involving antibodies and antigens) and "assay" (meaning to determine how much of something is present).
- Two major requirements for an immunoassay are:
- An antibody
- A detection system
Learn about the principles of immunoassay, a laboratory technique used to measure the concentration of antibodies or antigens in a sample. Understand the specific binding affinity between antibodies and antigens and the two major requirements for an immunoassay.
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