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Questions and Answers
What does the term 'immunoassay' literally mean?
What does the term 'immunoassay' literally mean?
- Immune system based measurement
- Measuring the immune system and an assay
- Measuring the immune response
- Immune system assay (correct)
What is the primary mechanism used in an immunoassay to quantify the target molecule?
What is the primary mechanism used in an immunoassay to quantify the target molecule?
- Specific binding affinity between an enzyme and substrate
- Specific binding affinity between a hormone and receptor
- Specific binding affinity between a protein and carbohydrate
- Specific binding affinity between an antibody and antigen (correct)
What are the two major requirements for an immunoassay?
What are the two major requirements for an immunoassay?
- An antibody and a detection system (correct)
- A hormone and a receptor
- An enzyme and a protein
- An antigen and a detection system
What is the purpose of an immunoassay?
What is the purpose of an immunoassay?
What is the function of an antibody in an immunoassay?
What is the function of an antibody in an immunoassay?
What enables immunoassays to distinguish between different antigens?
What enables immunoassays to distinguish between different antigens?
What is the role of an antibody in an immunoassay?
What is the role of an antibody in an immunoassay?
What is being measured in an immunoassay?
What is being measured in an immunoassay?
Why are antibodies used in immunoassays?
Why are antibodies used in immunoassays?
What is a necessary component of an immunoassay, in addition to an antibody?
What is a necessary component of an immunoassay, in addition to an antibody?
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Study Notes
Immunoassay
- Immunoassay is a diagnostic technique that combines the principles of the immune system and assay.
- The term "immuno" refers to the immune system, which involves components like antibodies and antigens.
- The term "assay" means to determine the quantity of a substance present.
- Immunoassay utilizes the specific binding affinity of an antibody for a specific antigen to quantify the amount of either the antibody or antigen present.
Requirements for Immunoassay
- Two major requirements for an immunoassay are:
- An antibody
- A detection system
Immunoassays
- Immunoassays exploit the specific affinity between antibodies and antigens to determine the quantity of either the antibody or antigen present.
- The term "immunoassay" is derived from "immuno" (relating to the immune system, involving antibodies and antigens) and "assay" (meaning to determine how much of something is present).
- Two major requirements for an immunoassay are:
- An antibody
- A detection system
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