26 Questions
What is the result of the interaction between the soluble antigen and antibody?
The formation of an insoluble lattice that forms precipitate out of the solution
What happens to the insoluble lattice formed in the interaction between the soluble antigen and antibody?
It precipitates out of the solution
What is the nature of the lattice formed in the interaction between the soluble antigen and antibody?
Insoluble
What is the fundamental component of the precipitate formed in the interaction between the soluble antigen and antibody?
Insoluble lattice
What is the underlying process that leads to the formation of the insoluble lattice in the interaction between the soluble antigen and antibody?
Antigen-antibody binding
What is the main characteristic of an antibody combining site?
The ability to react with only one antigenic determinant
What is the term for the ability of an antibody to react with only one antigenic determinant?
Specificity
What is an example of a pathogen that can be detected by IgM antibodies?
Cytomegalovirus
What type of antibody is produced first in response to an infection?
IgM
What is the relationship between an antibody and its antigenic determinant?
One antibody can bind to one antigenic determinant
What is one of the uses of serological means?
Detection of autoantibody
What can serological means measure?
The minute amount of hormones
What is another application of serological means?
Immunoassay of hormones
What is another use of serological means in pharmacology?
Detection of offending drugs
What is the primary advantage of serological means?
They are highly sensitive
What is the condition necessary for precipitation to occur?
Concentrations of antigen and antibody are equal
What happens if the concentration of antigen is in excess?
No precipitation occurs
What is the result of a deficient concentration of antibody?
No precipitation occurs
Under what condition does precipitation occur?
When concentrations of antigen and antibody are equivalent
What happens when the concentration of antigen is equal to the concentration of antibody?
Precipitation occurs
What is the relationship between Ab and Ag?
Ab is specific to Ag and there is no similar Ag
What happens when Ab and Ag are mixed together in a petri dish?
Two precipitation lines are formed
What is the purpose of incubation in the ELISA method?
To allow the reaction to occur between Ab and Ag
What can cause false positive results in the ELISA method?
Using a non-specific Ab
What is the purpose of using agar in the ELISA method?
To provide a solid medium for the reaction to occur
What is the result of mixing Ab with multiple Ag in the ELISA method?
Multiple precipitation lines are formed
This quiz covers the detection of autoantibodies, identification of offending drugs, and immunoassay of hormones, which enables the measurement of minute amounts of hormones.
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