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Questions and Answers
What is the purpose of the separation step in a labeled immunoassay?
What is the purpose of the separation step in a labeled immunoassay?
- To quantify the amount of binding
- To wash the solid-phase vehicle
- To remove unbound analyte (correct)
- To detect the label
What is the purpose of Anti-Human Globulin (AHG) in an indirect non-competitive EIA?
What is the purpose of Anti-Human Globulin (AHG) in an indirect non-competitive EIA?
- To remove unbound analytes
- To activate the label
- To bind to exposed antibodies (correct)
- To attach antibodies to antigens
In a competitive EIA, what happens when all competitor antigens (px Ags) are already bound to antibodies?
In a competitive EIA, what happens when all competitor antigens (px Ags) are already bound to antibodies?
- Labelled antigen binds to free antibodies (correct)
- Labelled antigen is activated
- Labelled antigen is washed off
- Labelled antigen binds to the solid phase
What is the key characteristic of Immunozymetric assays compared to direct EIA?
What is the key characteristic of Immunozymetric assays compared to direct EIA?
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Which label is commonly used in Fluorescence Immunoassays?
Which label is commonly used in Fluorescence Immunoassays?
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What is the chief disadvantage of Radioimmunoassay?
What is the chief disadvantage of Radioimmunoassay?
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What label is most popularly used in Radioimmunoassay?
What label is most popularly used in Radioimmunoassay?
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