Immune-Mediated Tissue Damage Quiz
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Questions and Answers

What is the purpose of the separation step in a labeled immunoassay?

  • To quantify the amount of binding
  • To wash the solid-phase vehicle
  • To remove unbound analyte (correct)
  • To detect the label

    • What is the purpose of Anti-Human Globulin (AHG) in an indirect non-competitive EIA?

  • To remove unbound analytes
  • To activate the label
  • To bind to exposed antibodies (correct)
  • To attach antibodies to antigens

    • In a competitive EIA, what happens when all competitor antigens (px Ags) are already bound to antibodies?

  • Labelled antigen binds to free antibodies (correct)
  • Labelled antigen is activated
  • Labelled antigen is washed off
  • Labelled antigen binds to the solid phase

    • What is the key characteristic of Immunozymetric assays compared to direct EIA?

    <p>Absence of solid-phase attachment for antibodies</p> Signup and view all the answers

    Which label is commonly used in Fluorescence Immunoassays?

    <p>FITC</p> Signup and view all the answers

    What is the chief disadvantage of Radioimmunoassay?

    <p>Radiation hazard</p> Signup and view all the answers

    What label is most popularly used in Radioimmunoassay?

    <p>$I^{125}$</p> Signup and view all the answers

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