Imaging Living Organs - Lecture 13

<p>It provides a more complex imaging of multiple proteins or structures (C)</p> Signup and view all the answers

<p>It allows for real-time visualization of cellular processes (A)</p> Signup and view all the answers

<p>Blue light (~480 nm) (C)</p> Signup and view all the answers

<p>Red (RFP) (D)</p> Signup and view all the answers

<p>They isolate specific wavelengths to improve detection of fluorescent signals (A)</p> Signup and view all the answers

<p>Aequorea victoria (jellyfish) (D)</p> Signup and view all the answers

<p>All of the above (D)</p> Signup and view all the answers

<p>Scanning Electron Microscopy (SEM) (D)</p> Signup and view all the answers

<p>Electron beam (B)</p> Signup and view all the answers

<p>It has a lower magnification. (B)</p> Signup and view all the answers

<p>Transmission Electron Microscopy (TEM) (B)</p> Signup and view all the answers

<p>~0.1 nm (C)</p> Signup and view all the answers

<p>Studying surface structures (A)</p> Signup and view all the answers

<p>Uses an electron beam to examine surfaces (B)</p> Signup and view all the answers

<p>It allows for greater resolution. (A)</p> Signup and view all the answers

<p>Transmission Electron Microscopy (TEM) (D)</p> Signup and view all the answers

<p>~1,000,000x (A)</p> Signup and view all the answers

The range of light visible to the human eye, spanning from violet (around 400 nm) to red (around 700 nm).

A technique that utilizes fluorescent molecules to visualize specific structures or components within a specimen. It involves exciting the molecules with specific wavelengths of light and capturing the emitted light at a longer wavelength.

The wavelength of light used to energize (excite) fluorescent molecules.

The wavelength of light emitted by an excited fluorescent molecule after absorbing energy.

A specialized microscope setup that uses a specific light source and filters to isolate and capture fluorescent signals from a specimen. It includes an excitation filter, dichroic mirror, and emission filter.

A mirror that reflects shorter wavelengths of light (e.g., excitation light) while allowing longer wavelengths (e.g., emitted light) to pass through.

A naturally occurring protein found in jellyfish that emits green fluorescence when exposed to blue light.

Modified versions of GFP that emit different colors of fluorescence, such as cyan (CFP), yellow (YFP), and red (RFP).

Fluorescence microscopy has diverse applications in biology, including identifying specific proteins and structures in cells, visualizing multiple components using different fluorescent markers, and studying gene expression and protein localization in living organisms.

Fluorescence microscopy allows researchers to visualize specific structures or components within a cell or organism with high sensitivity and detail. It offers a powerful tool to study living organisms and their internal processes without disrupting them.

A type of microscopy that uses electrons instead of light to image specimens, providing much higher resolution than light microscopy.

Electrons pass through a thin specimen, allowing us to visualize internal structures like organelles and viruses. Dense areas block electrons, appearing dark, while less dense areas allow electrons to pass, appearing light.

Electrons are scanned across the surface of a specimen, creating a 3D image by detecting scattered electrons. It reveals detailed surface structures.

A 2D image that reveals the internal structures of a specimen, showing details like organelles and viruses.

A 3D image that shows the surface of a specimen, revealing fine details like the texture of a blood cell.

The ability of a microscope to distinguish between two closely spaced objects. Higher resolution means you can see finer details.

The degree to which an image is enlarged compared to its actual size.

A condition where there is almost no air, required for electron microscopy because electrons can be easily deflected by air molecules.

The process of preparing a sample for electron microscopy, which involves fixing, dehydrating, and embedding the specimen.