Identifying Gram Positive and Gram Negative Bacteria

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12 Questions

How can you differentiate between gram positive and gram negative bacteria on a slide?

Positive bacteria retain crystal violet dye, while negative bacteria do not retain the dye.

What color does a gram negative bacterium appear on a slide after washing?

Pinkish

Which bacterium is identified using acid-fast stain instead of gram staining?

Mycobacterium tuberculosis

What is the significance of Coagulase positive in bacterial identification?

It indicates an infection caused by Staphylococcus aureus.

What is the main function of fimbriae in bacteria?

Attachment

Which virulence factor enables swimming for protozoa, bacteria, and spermatozoa?

Flagellum

What is the function of human IgG antibodies binding by Staph aureus?

To provide disguise

Which type of toxins are produced by both Gram-positive and Gram-negative cells?

Cytotoxins

What is the function of exotoxins produced by bacteria like Staph aureus?

Inhibit protein synthesis

Where is Lipid A portion, which is highly immunoreactive, found in Gram-negative bacteria?

Exposed on the cell membrane surface

What is the main impact of endotoxins when released into the blood upon bacterial death?

Massive inflammatory response

Which specific bacteria have the ability to form endospores that are hard to kill?

Clostridium tetani and Bacillus anthracis

Learn how to differentiate between gram positive and gram negative bacteria based on the retention of crystal violet dye on a slide. Understand the characteristics of cell walls in both types of bacteria.

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