17 Questions
What is the size of Babesia bovis, a small babesia species?
2.4 × 1.5 μm
What is the characteristic shape of Babesia bigemina in blood smears?
Pear-shaped bodies joined at an acute angle within the red blood cell
What is the purpose of adding one drop of 0.1% methylene blue to the blood sample?
Staining
What is the effect of Anaplasma spp. on red blood cells?
Mass destruction of RBC’s
What is the characteristic feature of Theileria equi in blood smears?
Divides into 4 daughter cells and forming Maltese cross
What is the reason for preparing blood smears as soon as possible after collection?
To prevent changes in parasite morphology and staining characteristics.
What is the purpose of using a cover-slip in the wet blood film technique?
To spread the blood as a monolayer of cells.
What is the advantage of using thick smears in parasite detection?
Increased sensitivity, allowing for more efficient detection of parasites.
What is the recommended action if a thick smear is positive for malaria parasites?
Use a thin smear for species identification.
What is the significance of dehemoglobinized red blood cells in thick smears?
They allow for increased sensitivity in parasite detection.
What is the purpose of centrifugation in the Haematocrit centrifugation technique?
To separate parasites from other blood components.
What is the recommended blood sample for detecting microfilariae, and what type of smears are prepared from it?
Capillary (fingerstick) blood, and thick and thin smears.
What is the purpose of centrifugation in Knott's technique for detecting microfilariae?
To concentrate the microfilariae and WBCs in the sediment.
What is the recommended lysing solution for use in filtration kits for detecting microfilariae?
2% formalin.
Why is it necessary to fix the blood smears with absolute methanol before Giemsa staining?
To enhance the staining of microfilariae.
What is the purpose of washing the filter with water in the filtration method for detecting microfilariae?
To remove remaining small debris.
What is the recommended pore size of the filter used in filtration kits for detecting microfilariae?
5-μm.
This quiz will test your knowledge on identifying and characterizing Babesia species, including Babesia bovis and Babesia bigemina, in blood smears. Learn to recognize the distinct shapes and sizes of these parasites and their characteristics under the microscope.
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