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Identifying Babesia Species in Blood Smears

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Identifying Babesia Species in Blood Smears

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Questions and Answers

What is the size of Babesia bovis, a small babesia species?

2.4 × 1.5 μm

What is the characteristic shape of Babesia bigemina in blood smears?

Pear-shaped bodies joined at an acute angle within the red blood cell

What is the purpose of adding one drop of 0.1% methylene blue to the blood sample?

Staining

What is the effect of Anaplasma spp. on red blood cells?

Mass destruction of RBC’s Signup and view all the answers

What is the characteristic feature of Theileria equi in blood smears?

Divides into 4 daughter cells and forming Maltese cross Signup and view all the answers

What is the reason for preparing blood smears as soon as possible after collection?

To prevent changes in parasite morphology and staining characteristics. Signup and view all the answers

What is the purpose of using a cover-slip in the wet blood film technique?

To spread the blood as a monolayer of cells. Signup and view all the answers

What is the advantage of using thick smears in parasite detection?

Increased sensitivity, allowing for more efficient detection of parasites. Signup and view all the answers

What is the recommended action if a thick smear is positive for malaria parasites?

Use a thin smear for species identification. Signup and view all the answers

What is the significance of dehemoglobinized red blood cells in thick smears?

They allow for increased sensitivity in parasite detection. Signup and view all the answers

What is the purpose of centrifugation in the Haematocrit centrifugation technique?

To separate parasites from other blood components. Signup and view all the answers

What is the recommended blood sample for detecting microfilariae, and what type of smears are prepared from it?

Capillary (fingerstick) blood, and thick and thin smears. Signup and view all the answers

What is the purpose of centrifugation in Knott's technique for detecting microfilariae?

To concentrate the microfilariae and WBCs in the sediment. Signup and view all the answers

What is the recommended lysing solution for use in filtration kits for detecting microfilariae?

2% formalin. Signup and view all the answers

Why is it necessary to fix the blood smears with absolute methanol before Giemsa staining?

To enhance the staining of microfilariae. Signup and view all the answers

What is the purpose of washing the filter with water in the filtration method for detecting microfilariae?

To remove remaining small debris. Signup and view all the answers

What is the recommended pore size of the filter used in filtration kits for detecting microfilariae?

5-μm. Signup and view all the answers