Hypersensitivity Testing: In Vitro Total IgE Testing Principles

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What is the main benefit of total IgE testing compared to skin testing?

It is less traumatic to the patient.

Which type of IgE testing is commonly used to aid in diagnosing allergic rhinitis and asthma?

Total IgE testing

What distinguishes newer antigen-specific IgE testing methods from the original RAST method?

They use enzyme or fluorescent labels instead of radioactivity.

Why is allergen-specific IgE testing considered safer than skin testing?

It is less traumatic to patients.

Which method is regarded as the gold standard for testing contact dermatitis?

Patch test

When should a patch test for contact dermatitis ideally be conducted?

When the patient is symptom-free

What is considered a positive reaction in the skin patch test described in the text?

Redness with papules or tiny blisters

What is the purpose of conducting the final evaluation of the skin patch test at 96 to 120 hours?

To identify late-phase reactions

Which type of hypersensitivity reaction is detected by the MELISA blood test mentioned in the text?

Type IV

How does the oral food challenge (OFC) proceed in terms of increasing allergen dose?

Each dose administers a larger amount of protein

What proportion of the population may be affected by Type IV hypersensitivity reactions to nickel according to the text?

20%

What is a possible cause for false negative results in skin testing as indicated in the text?

Inadequate contact with the skin during testing

Explore the principles of in vitro total IgE testing for hypersensitivity. Learn about the importance of total IgE testing in screening patients before referral to an allergy specialist and its clinical use in diagnosing allergic conditions like rhinitis and asthma.

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