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Data are from one of four (a) or two (c) independent experiments with similar results or represent two (b,d) on which responder T cells depend for independent experiments.
Data are from one of four (a) or two (c) independent experiments with similar results or represent two (b,d) on which responder T cells depend for independent experiments.
sets
In addition to causing cell death, early stimulation, a time point that preceded the onset of apoptosis. The cytokine consumption may also disrupt the positive cytokine feedback unaltered GFP expression in responder T cells showed that ______ loop that promotes the late cytokine production by viable cells transcription was unaffected by Treg cells (Fig. 4c).
In addition to causing cell death, early stimulation, a time point that preceded the onset of apoptosis. The cytokine consumption may also disrupt the positive cytokine feedback unaltered GFP expression in responder T cells showed that ______ loop that promotes the late cytokine production by viable cells transcription was unaffected by Treg cells (Fig. 4c).
Il2
Finally, we engaged in an immune response. Indeed, we found that responder quantified intracellular IL-2 protein in responder T cells that were T cells isolated after 3 d of culture together with Treg cells had much cultured together with Treg or control T cells for 24 h and then less expression of the cytokine receptors CD25 and ______ (common restimulated with phorbol myristate acetate (PMA) and ionomycin.g-chain), indicating that either effector T cells expressing CD25 and Neither Treg nor control T cells altered the IL-2 protein expression by CD132 were killed or the positive feedback loop by which IL-2 responder T cells (Fig. 4d).
Finally, we engaged in an immune response. Indeed, we found that responder quantified intracellular IL-2 protein in responder T cells that were T cells isolated after 3 d of culture together with Treg cells had much cultured together with Treg or control T cells for 24 h and then less expression of the cytokine receptors CD25 and ______ (common restimulated with phorbol myristate acetate (PMA) and ionomycin.g-chain), indicating that either effector T cells expressing CD25 and Neither Treg nor control T cells altered the IL-2 protein expression by CD132 were killed or the positive feedback loop by which IL-2 responder T cells (Fig. 4d).
CD132
Because restimulation with PMA and maintains receptor expression at late time points was impaired ionomycin may overcome Treg cell–mediated suppression10, we also (Fig. 5e). Nonetheless, exogenous IL-7 restored the expression of ______ cells with anti-CD3 and obtained similar results (Fig. 4e).
Because restimulation with PMA and maintains receptor expression at late time points was impaired ionomycin may overcome Treg cell–mediated suppression10, we also (Fig. 5e). Nonetheless, exogenous IL-7 restored the expression of ______ cells with anti-CD3 and obtained similar results (Fig. 4e).
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Why were IL-2 concentrations much lower in supernatants of cocultures of responder T cells and Treg cells compared to cocultures of responder T cells and control T cells?
Why were IL-2 concentrations much lower in supernatants of cocultures of responder T cells and Treg cells compared to cocultures of responder T cells and control T cells?
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What was the purpose of measuring early steady-state Il2 mRNA expression in responder T cells isolated from cocultures of responder T cells and Treg cells?
What was the purpose of measuring early steady-state Il2 mRNA expression in responder T cells isolated from cocultures of responder T cells and Treg cells?
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In cocultures of Treg cells and responder T cells, what was the result regarding IL-2 production by responder T cells compared to when they were stimulated alone?
In cocultures of Treg cells and responder T cells, what was the result regarding IL-2 production by responder T cells compared to when they were stimulated alone?
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What was measured using flow cytometry in cocultures of responder T cells?
What was measured using flow cytometry in cocultures of responder T cells?
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What does the reduced expression of cytokine receptors CD25 and CD132 in responder T cells cultured with Treg cells indicate?
What does the reduced expression of cytokine receptors CD25 and CD132 in responder T cells cultured with Treg cells indicate?
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Why did responder T cells isolated after 3 days of culture with Treg cells show less expression of CD25 and CD132 receptors?
Why did responder T cells isolated after 3 days of culture with Treg cells show less expression of CD25 and CD132 receptors?
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'Knock-in' GFP expression was used to measure what aspect in responder T cells during coculture experiments?
'Knock-in' GFP expression was used to measure what aspect in responder T cells during coculture experiments?
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