Human Histology: Nervous Tissue Lab

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Questions and Answers

For faster action potential propagation within the CNS, axons are myelinated and insulated by ______.

oligodendrocytes

In the CNS, the type of neuroglia that replicates to occupy the space of dying neurons is the ______.

astrocyte

The brain ventricles are lined by ______ cells that also contribute to the production of cerebrospinal fluid.

ependymal

Within the brain and spinal cord, ______ act as phagocytic cells that remove waste and protect against harmful substances.

<p>microglia</p> Signup and view all the answers

The ______ forms a part of the blood-brain barrier, limiting what substances can enter the CNS.

<p>astrocyte</p> Signup and view all the answers

The outermost layer of the meninges, known as the ______, is composed of thick, dense irregular connective tissue.

<p>dura mater</p> Signup and view all the answers

Located between the dura mater and the pia mater, the ______ features a system of loosely arranged trabeculae.

<p>arachnoid mater</p> Signup and view all the answers

The ______, consisting of flattened mesenchymal-derived cells, is located innermost of the meningeal layers.

<p>pia mater</p> Signup and view all the answers

The cerebrum's outer region, known as ______, is mainly composed of neuronal cell bodies.

<p>gray matter</p> Signup and view all the answers

The cerebrum's inner tissue, known as ______, consists mostly of myelinated nerve fibers.

<p>white matter</p> Signup and view all the answers

In the spinal cord, interneurons are found within the two ______.

<p>dorsal horns</p> Signup and view all the answers

Located peripherally, the ______ of the spinal cord mainly contain ascending and descending myelinated fibers.

<p>white matter</p> Signup and view all the answers

In the spinal cord, multipolar motor neurons are located in the two ______.

<p>ventral horns</p> Signup and view all the answers

In the cerebral cortex, the ______ layer is characterized by a high density of small pyramidal cells.

<p>external granular</p> Signup and view all the answers

The ______ layer of the cerebral cortex is notable for containing medium-sized pyramidal cells.

<p>external pyramidal</p> Signup and view all the answers

Deeper within the cerebral cortex, the ______ layer is characterized by its large pyramidal cells.

<p>internal pyramidal</p> Signup and view all the answers

The innermost cortical layer, known as the ______ layer, consists of a diverse population of cells.

<p>multiform</p> Signup and view all the answers

The ______ cell layer of the cerebellar cortex is situated between the molecular and granular layers.

<p>Purkinje</p> Signup and view all the answers

In the cerebellar cortex, densely packed ______ cells form the innermost layer.

<p>granule</p> Signup and view all the answers

The outermost layer of the cerebellar cortex is the ______ layer.

<p>molecular</p> Signup and view all the answers

In the peripheral nervous system, ______ cells surround and insulate axons for faster action potential speed.

<p>schwann</p> Signup and view all the answers

[Blank] cells electrically insulate PNS cell bodies and regulate nutrient and waste exchange in ganglia.

<p>satellite</p> Signup and view all the answers

The external coat of a nerve, comprising dense connective tissue, is referred to as the ______.

<p>epineurium</p> Signup and view all the answers

Bundles of nerve fibers are surrounded by ______, a protective connective tissue layer.

<p>perineurium</p> Signup and view all the answers

Each individual nerve fiber is surrounded by the ______, providing insulation and support.

<p>endoneurium</p> Signup and view all the answers

Nodes of Ranvier and internodal segments are characteristics of ______ nerve fibers.

<p>myelinated</p> Signup and view all the answers

Unlike myelinated fibers, ______ nerve fibers feature smaller diameter axons and lack multiple wrappings; nodes of ranvier are not seen. They are still supported by Schwann cells.

<p>unmyelinated</p> Signup and view all the answers

[Blank] are equivalent to the rough endoplasmic reticulum.

<p>nissl bodies</p> Signup and view all the answers

A ______ is a neuron with a single axon and multiple dendrites.

<p>multipolar neuron</p> Signup and view all the answers

[Blank] is the lipoidal substance that covers the nerve fibers in the CNS and PNS.

<p>myelin</p> Signup and view all the answers

The ______ is the germ layer where the nervous tissue is derived.

<p>ectoderm</p> Signup and view all the answers

In the brain, gray matter is located in the ______ region and white matter is located in the ______ region; In the spinal cord, gray matter is located in the ______ region and the white matter is located in the ______ region.

<p>outer, inner, inner, outer</p> Signup and view all the answers

[Blank] is the phagocyte of the nervous tissue.

<p>microglia</p> Signup and view all the answers

The ______ is the functional unit of a nervous tissue.

<p>neuron</p> Signup and view all the answers

A ______ is a bundle of axons in the peripheral nervous system (PNS), while a ______ is a bundle of axons in the central nervous system (CNS).

<p>nerve, tract</p> Signup and view all the answers

A ______ is a collection of neuron cell bodies outside the CNS, while a ______ is a collection of neuron cell bodies inside the CNS.

<p>ganglion, nucleus</p> Signup and view all the answers

A ______ ganglion has neuron cell bodies in which processes extend to peripheral tissues while the ______ ganglion is only visceral motor neurons.

<p>spinal, autonomic</p> Signup and view all the answers

The types of neurons can be differentiated via the number of processes of their ______. Multipolar neurons have many; bipolar have two; unipolar have one.

<p>cell body</p> Signup and view all the answers

The differences between gray matter in the cerebrum versus the cerebellum is the number of relative ______ and ______.

<p>neurons, neuroglia</p> Signup and view all the answers

The three components of the peripheral nervous system consist of ______, ______, and ______.

<p>nerves, ganglia, nerve endings</p> Signup and view all the answers

The part of the neuron that typically transmits signals away from the cell body is the ______.

<p>axon</p> Signup and view all the answers

The type of neuron with one axon and multiple dendrites is classified as a ______ neuron.

<p>multipolar</p> Signup and view all the answers

[Blank] cells in the central nervous system are responsible for myelinating axons, which speeds up signal transmission.

<p>Oligodendrocytes</p> Signup and view all the answers

The brain and spinal cord are the main components of the ______ nervous system.

<p>central</p> Signup and view all the answers

Within the cerebral cortex, the layer rich in small, densely packed neurons is known as the external ______ layer.

<p>granular</p> Signup and view all the answers

The ______ mater is the meningeal layer directly in contact with the brain and spinal cord.

<p>pia</p> Signup and view all the answers

The outermost layer of the three meninges surrounding the brain and spinal cord is known as the ______ mater.

<p>dura</p> Signup and view all the answers

A key function of ______, a type of glial cell, is to phagocytize and remove cellular debris in the nervous tissue.

<p>microglia</p> Signup and view all the answers

The cerebral cortex is organized into six layers, with layer I being identified as the ______ layer.

<p>molecular</p> Signup and view all the answers

Unlike oligodendrocytes, ______ Cells myelinate nerve fibers in the peripheral nervous system, enabling faster nerve impulse conduction.

<p>Schwann</p> Signup and view all the answers

The dorsal and ventral ______ are key structures in the spinal cord's gray matter, with the dorsal horns receiving sensory information.

<p>horns</p> Signup and view all the answers

The abundant glial cells known as ______ have perivascular feet that contribute to the blood-brain barrier.

<p>astrocytes</p> Signup and view all the answers

The area of the brain known as the ______ has a distinct histology, featuring a molecular layer, Purkinje cell layer, and granular layer.

<p>cerebellum</p> Signup and view all the answers

Areas of the central nervous system predominantly composed of myelinated axons are referred to as ______ matter.

<p>white</p> Signup and view all the answers

A ______ neuron is characterized by a single axon and a single dendrite extending from opposite sides of the cell body.

<p>bipolar</p> Signup and view all the answers

A ______ is a junction between two neurons where neurotransmitters are released to facilitate communication.

<p>synapse</p> Signup and view all the answers

The ______ cells are ciliated and line the ventricles of the brain, aiding in the production and circulation of cerebrospinal fluid (CSF).

<p>ependymal</p> Signup and view all the answers

The covering of connective tissue that surrounds a nerve fascicle is called the ______.

<p>perineurium</p> Signup and view all the answers

Groups of neuronal cell bodies located outside the central nervous system are called ______.

<p>ganglia</p> Signup and view all the answers

The nodes of ______ are gaps in the myelin sheath that expose the axon membrane to facilitate rapid nerve impulse conduction.

<p>Ranvier</p> Signup and view all the answers

The germ layer from which nervous tissue is derived during embryonic development is the ______.

<p>ectoderm</p> Signup and view all the answers

The functional unit of nervous tissue is the ______.

<p>nerve cell</p> Signup and view all the answers

[Blank] are equivalent to the rough endoplasmic reticulum in neurons and are the sites of protein synthesis.

<p>Nissl bodies</p> Signup and view all the answers

A neuron with a single axon and multiple dendrites is a ______ neuron.

<p>multipolar</p> Signup and view all the answers

The lipoidal substance that covers nerve fibers in the CNS and PNS is called ______.

<p>myelin</p> Signup and view all the answers

Flashcards

Neuron

A nerve cell specialized for communication. It receives, processes, and transmits information to other cells.

Dendrites

Branch-like extensions of a neuron that receive signals from other neurons. They increase the surface area available for receiving these signals.

Axon hillock

Area where the axon exits the neuron cell body.

Axon

A long, slender projection of a nerve cell, or neuron, that conducts electrical impulses away from the neuron's cell body.

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Synapse

A junction between two nerve cells, consisting of a minute gap across which impulses pass by diffusion of a neurotransmitter

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Multipolar neuron

A neuron with one axon and multiple dendrites; the most common type of neuron in the central nervous system.

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Bipolar neuron

A neuron with one axon and one dendrite.

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Unipolar Neuron

A neuron that has a single process extending from the cell body, which then branches into two axons.

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Glial Cells

Supporting cells found in nervous tissue that are non-neuronal and provide support and protection for neurons.

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Central Nervous System

The brain and spinal cord make up this system.

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Astrocytes

A type of glial cell found in the CNS that forms part of the blood-brain barrier and regulates the composition of interstitial fluid.

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Oligodendrocytes

A type of glial cell in the CNS responsible for myelinating axons.

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Microglia

A type of glial cell in the CNS that acts as a phagocyte, removing debris and pathogens.

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Ependymal cells

Glial cells that line the ventricles of the brain and central canal of the spinal cord, assisting in the production and circulation of cerebrospinal fluid.

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Schwann cells

These are specialized glial cells that myelinate axons in the peripheral nervous system.

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Satellite cells

Cells surrounding neuron cell bodies in ganglia; they regulate the microenvironment and provide electrical insulation.

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Dura mater

The thick, outermost layer of the meninges, made of dense irregular connective tissue.

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Arachnoid mater

The middle layer of the meninges, characterized by a web-like structure with loosely arranged trabeculae.

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Pia mater

The innermost layer of the meninges, directly adhering to the surface of the brain and spinal cord.

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White Matter

Area of brain tissue that is mostly myelinated.

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Gray Matter

Area of brain tissue that is mostly neuronal cell bodies, unmyelinated fibers, and neuroglial cells.

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Cerebrum

The largest part of the brain, responsible for higher-level cognitive functions.

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Cerebellum

A brain structure involved in motor control and coordination, as well as some cognitive functions.

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Dorsal Horns

Regions of gray matter within the spinal cord that contain interneurons receiving sensory fibers.

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Ventral Horns

Regions of gray matter in the spinal cord that contain multipolar motor neurons.

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Gray Matter

The outer region of the brain.

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Ectoderm

A germ layer where the nervous tissue is derived.

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Neuron

The functional unit of a nervous tissue.

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Nissl Substance

Also known as Nissl bodies, they are equivalent to the rough endoplasmic reticulum.

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Myelin

The lipoidal substance that covers the nerve fibers in the CNS and PNS.

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Ganglion

A collection of neuron cell bodies outside the central nervous system.

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Nerve (PNS)

A bundle of axons in the peripheral nervous system.

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Tract (CNS)

A bundle of axons in the central nervous system.

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Epineurium

External coat of nerve.

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Perineurium

the outermost layer that surrounds each bundle of nerve.

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Endoneurium

innermost layer that surrounds individual nerve fiber.

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Satellite cells

Cells surrounding neuron cell bodies in ganglia

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Nodes of ranvier

Areas of myelin sheath

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Myelinated Nerve Fibers

Schwann cells + Myelin sheath = ?

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Unmyelinated Nerve Fibers

Bundles of nerve fibers.

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Study Notes

  • The topic is Unit 4: Nervous Tissue for Human Histology (Laboratory) MT120225
  • The semester is Second Semester A.Y. 2024-2025
  • The nervous system is being studied at UST General Santos - School of Health Sciences, Department of Medical Technology

Learning Outcomes

  • Differentiate neuron types by structure.
  • Identify neuroglial cells in the central nervous system, (CNS) and peripheral nervous system, (PNS).
  • Distinguish brain and spinal cord regions by location and structural components.
  • Identify cerebellum's histologic layers.
  • Differentiate myelinated and unmyelinated nerve fibers microscopically.

Overview of Nervous Tissue

Morphology of a Typical Neuron

  • Neurons have dendrites, multipolar neurons, Nissl substance, axon hillock, axons, arterioles, neuroglia, a nucleus, and a nucleolus.
  • In the anterior horn of the human spinal cord one can observe neuroglial nuclei and Nissl bodies when stained with Toluidine Blue.
  • Silver Impregnated Purkinje Neurons show dendrites in the Cerebellum.

Types of Neurons

  • Neurons are classified into structural classes.
  • Multipolar neurons have multiple dendrites and one axon.
  • Bipolar neurons have one dendrite and one axon.
  • Unipolar neurons arise from a single short process.
  • Pseudounipolar neurons are a class of neurons
  • Anaxonic neurons have many dendrites but no axon.

Classifications of Neurons

  • Pseudounipolar neurons have satellite cells and cell bodies of sensory neurons.
  • Bipolar neurons have axon hillocks, soma, neuroglia, and dendrites.
  • Multipolar neurons have axons, axon hillocks, soma, dendrites, nuclei, and nucleolus.

Synapse

  • Acetylcholine binds to receptor protein, causing ion gates to open
  • Postsynaptic neurons have Sodium (Na+) ions

Central Nervous System

Glial Cells of the CNS

  • Astrocytes form the blood-brain barrier and regulate interstitial fluid composition.
  • Astrocytes provide structural support, aid neural development and occupy dying neuron space
  • Oligodendrocytes myelinate CNS axons, enabling faster action potential propagation.
  • Microglia are phagocytic cells, protecting the CNS by engulfing infectious agents.
  • Ependymal cells line brain ventricles and the spinal cord's central canal.
  • Ependymal cells produce and circulate cerebrospinal fluid (CSF).
  • Protoplasmic and fibrous structures are central neuroglia, astrocytes.
  • Anti-GFAP Antibodies are used in the immunohistochemical staining of astrocytes in brain white matter

Connective Tissue of the CNS / Meningeal Layers

  • Dura Mater (outermost): Thick dense irregular connective tissue that is continuous with the periosteum of the skull.
  • Arachnoid Mater (middle): Has two components being a sheet of connective tissue in contact with the dura mater and a system of loosely arranged trabeculae continuous with the pia mater.
  • Pia Mater (innermost): Consists of flattened mesenchymal-derived cells.
  • The meninges consist of the Dura mater, arachnoid mater and the pia mater.

Structures of the Central Nervous System

  • White matter consists mostly of myelinated nerve fibers.
  • White matter contains some unmyelinated fibers and glial cells.
  • Gray matter consists mostly of neuronal cell bodies.
  • Gray matter contains unmyelinated fibers and neuroglial cells.

Cerebrum

  • The cerebrum is part of the central nervous system.
  • The cerebrum has structures such as pia mater, gray matter, and white matter.

Cerebral Cortex

  • The cerebral cortex has a molecular layer, external granular layer, external pyramidal layer, internal granular layer, internal pyramidal layer, and multiform layer
  • Key components are Pia mater with blood vessels, neuroglial cells, small pyramidal cells, apical dendrites of pyramidal cells, medium-sized pyramidal cells, granule cells, dendrites of pyramidal cells, large pyramidal cells, and bundles of axons

Cerebellum

  • The Cerebellum consists of the cerebellar cortex, the molecular layer granule cell layer, and white matter
  • The Cerebellar Cortex consists of basket cells, dendrites of Purkinje cells, Purkinje cells with nucleus and nucleolus, Granular cell layer, white matter, Molecular cell layer, Purkinje cell layer, Golgi type II cells, Granule cells, Glomeruli and Axons

Spinal Cord

  • Gray Matter (H shaped): 2 dorsal horns contain interneurons (receives sensory fibers from neurons in the spinal cord, 2 ventral horns contain multipolar motor neurons
  • White Matter: Peripherally located and consists of ascending and descending fibers (mostly myelinated)

Peripheral Nervous System

  • Nerve, ganglia, and nerve endings are components.

Glial Cells of the Peripheral Nervous System

  • Schwann cells surround and insulate PNS axons.
  • Schwann cells myelinate PNS axons, enabling faster action potential propagation.
  • Satellite cells electrically insulate PNS cell bodies and regulate nutrient/waste exchange.

Peripheral Neuroglia

  • Schwann Cells: Myelinated Nerve Fibers, Schwan cells, the Axon and the Myelin sheath
  • Satellite Cells are located around neurons of ganglia in the PNS.

Connective Tissue of the PNS

  • Epineurium is the external coat of the nerve.
  • Perineurium surrounds each bundle of nerve.
  • Endoneurium surrounds individual nerve fibers.

Peripheral Nerve

  • Peripheral nerves have axons and myelin sheaths.

Ganglion

  • Ganglion have blood vessels

Myelinated Nerve Fibers

  • Myelinated nerve fibers are enclosed by a myelin sheath
  • Myelinated nerve fibers prevent loss of nerve impulse
  • Circular constrictions on myelinated nerve fibers are nodes of Ranvier
  • Internodal segments or schwann segments are components of myelinated nerve fibers.

Unmyelinated Nerve Fibers

  • Unmyelinated nerve fibers have naked axons.
  • Have no multiple wrapping to form a myelin sheath
  • Consist of smaller diameter axons
  • Still consist of schwann cells
  • Nodes of ranvier are not seen

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