Podcast
Questions and Answers
What percentage of whole blood is constituted by formed elements?
What percentage of whole blood is constituted by formed elements?
- 55%
- 45% (correct)
- 8.5%
- 7%
What is the normal pH range of human arterial blood?
What is the normal pH range of human arterial blood?
- 7.45-7.55
- 7.35-7.45 (correct)
- 7.40-7.50
- 7.25-7.35
Which plasma protein constitutes the largest percentage of plasma proteins?
Which plasma protein constitutes the largest percentage of plasma proteins?
- Globulins
- Albumins (correct)
- Immunoglobulins
- Fibrinogen
What shape do mature red blood cells take?
What shape do mature red blood cells take?
Which of the following statements is true about blood plasma?
Which of the following statements is true about blood plasma?
How much blood volume does an average adult human have?
How much blood volume does an average adult human have?
What role do plasma proteins play in the body?
What role do plasma proteins play in the body?
Where are red blood cells primarily formed in adults?
Where are red blood cells primarily formed in adults?
What is the primary focus of the lecture notes authored by Yared Alemu and others?
What is the primary focus of the lecture notes authored by Yared Alemu and others?
Which organizations collaborated in the production of the lecture notes?
Which organizations collaborated in the production of the lecture notes?
How many chapters are included in the lecture notes?
How many chapters are included in the lecture notes?
What type of permissions is granted for printing and photocopying the lecture notes?
What type of permissions is granted for printing and photocopying the lecture notes?
What is the intended audience for these lecture notes?
What is the intended audience for these lecture notes?
What do the chapters in the lecture notes begin with?
What do the chapters in the lecture notes begin with?
What problem are the authors trying to address with these lecture notes?
What problem are the authors trying to address with these lecture notes?
What is prohibited under the guidelines for using these lecture notes?
What is prohibited under the guidelines for using these lecture notes?
Where does hemopoiesis first establish during fetal life?
Where does hemopoiesis first establish during fetal life?
At what point does bone marrow completely take over hemopoiesis from the liver?
At what point does bone marrow completely take over hemopoiesis from the liver?
Which bones are primarily involved in hemopoiesis from infancy to adulthood?
Which bones are primarily involved in hemopoiesis from infancy to adulthood?
What role do extracellular matrix components like fibronectin and collagen play in hemopoiesis?
What role do extracellular matrix components like fibronectin and collagen play in hemopoiesis?
What stimulates the proliferation of erythrocyte precursors?
What stimulates the proliferation of erythrocyte precursors?
Which cells are responsible for producing various cytokines that regulate hematopoiesis?
Which cells are responsible for producing various cytokines that regulate hematopoiesis?
How does the body respond to increased demands for blood cells?
How does the body respond to increased demands for blood cells?
What separates the extravascular marrow compartment from the intravascular compartment?
What separates the extravascular marrow compartment from the intravascular compartment?
What is the typical size of a mature basophil?
What is the typical size of a mature basophil?
What characteristic helps to identify a monoblast under histochemical criteria?
What characteristic helps to identify a monoblast under histochemical criteria?
Which of the following is true regarding eosinophils in deficiency states?
Which of the following is true regarding eosinophils in deficiency states?
What is a distinguishing feature of the promonocyte?
What is a distinguishing feature of the promonocyte?
How would you describe the cytoplasm of mature monocytes?
How would you describe the cytoplasm of mature monocytes?
What type of nuclear shape can be observed in mature eosinophils?
What type of nuclear shape can be observed in mature eosinophils?
What is the nuclear/cytoplasmic ratio in a promonocyte?
What is the nuclear/cytoplasmic ratio in a promonocyte?
Which feature differentiates basophils from eosinophils in terms of their granules?
Which feature differentiates basophils from eosinophils in terms of their granules?
What is the main purpose of a thin blood film in hematology?
What is the main purpose of a thin blood film in hematology?
Which statement best describes the preparation technique for a thin blood film?
Which statement best describes the preparation technique for a thin blood film?
What is a potential effect of using a blood sample that has been kept at room temperature for an extended period on blood cell morphology?
What is a potential effect of using a blood sample that has been kept at room temperature for an extended period on blood cell morphology?
Which dye mixture was developed by Romanowsky for staining blood cells?
Which dye mixture was developed by Romanowsky for staining blood cells?
What color does eosin stain in blood films due to its acidic nature?
What color does eosin stain in blood films due to its acidic nature?
What characterizes the staining properties of methylene blue in Romanowsky stains?
What characterizes the staining properties of methylene blue in Romanowsky stains?
Which of the following is NOT a desirable quality of a thin blood film?
Which of the following is NOT a desirable quality of a thin blood film?
What problem may arise during the staining of blood films?
What problem may arise during the staining of blood films?
What color are the nuclei of leucocytes when viewed in a Romanowsky-stained blood film?
What color are the nuclei of leucocytes when viewed in a Romanowsky-stained blood film?
Which granules are distinctly red orange in color in a Romanowsky-stained blood film?
Which granules are distinctly red orange in color in a Romanowsky-stained blood film?
What is the diluent used for Giemsa stain in buffered distilled water?
What is the diluent used for Giemsa stain in buffered distilled water?
During the Jenner-Giemsa staining method, how long should the films be immersed in methanol for fixation?
During the Jenner-Giemsa staining method, how long should the films be immersed in methanol for fixation?
Which component of the blood film has a sky blue cytoplasm and red purple chromatin when infected with malaria?
Which component of the blood film has a sky blue cytoplasm and red purple chromatin when infected with malaria?
What is the main purpose of panoptic staining?
What is the main purpose of panoptic staining?
What color are platelet granules in a Romanowsky-stained blood film?
What color are platelet granules in a Romanowsky-stained blood film?
What should be done after staining with Giemsa stain in the preparation process?
What should be done after staining with Giemsa stain in the preparation process?
Flashcards
Lack of Reference Materials
Lack of Reference Materials
The shortage of adequate and consistent materials for learning, particularly in medical laboratory technology courses in Ethiopia.
Non-Uniform Course Syllabi
Non-Uniform Course Syllabi
The lack of uniform course content across different institutions, resulting in inconsistent learning experiences for students.
Purpose of Lecture Notes
Purpose of Lecture Notes
The goal of the lecture notes is to address the existing problems of insufficient resources and inconsistent learning materials.
Target Audience
Target Audience
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Structure of Lecture Notes
Structure of Lecture Notes
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Learning Objectives
Learning Objectives
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Background Information
Background Information
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Chapter Review Questions
Chapter Review Questions
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Blood Plasma
Blood Plasma
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Formed elements
Formed elements
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Erythrocytes
Erythrocytes
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Hemoglobin
Hemoglobin
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Leukocytes
Leukocytes
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Platelets (Thrombocytes)
Platelets (Thrombocytes)
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Hematopoiesis
Hematopoiesis
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pH of blood
pH of blood
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Bone Marrow
Bone Marrow
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Hematopoietic Transition
Hematopoietic Transition
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Reticulin Framework
Reticulin Framework
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Hematopoietic Growth Factors
Hematopoietic Growth Factors
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Erythropoietin (EPO)
Erythropoietin (EPO)
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Thrombopoietin
Thrombopoietin
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Cytokines
Cytokines
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Eosinophil
Eosinophil
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Basophil
Basophil
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Promonocyte
Promonocyte
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Monocyte
Monocyte
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Macrophage
Macrophage
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Monoblast
Monoblast
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Auer bodies
Auer bodies
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Thin blood film
Thin blood film
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Blood film preparation
Blood film preparation
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Desirable qualities of a thin blood film
Desirable qualities of a thin blood film
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Effect of room temperature blood sample
Effect of room temperature blood sample
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Romanowsky staining
Romanowsky staining
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Principle of staining
Principle of staining
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Thick blood film
Thick blood film
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Panoptic stains
Panoptic stains
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Red Blood Cells (RBCs)
Red Blood Cells (RBCs)
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Leukocyte Nuclei
Leukocyte Nuclei
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Neutrophilic Granules
Neutrophilic Granules
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Eosinophilic Granules
Eosinophilic Granules
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Basophilic Granules
Basophilic Granules
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Platelets
Platelets
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Study Notes
LECTURE NOTES
- For Medical Laboratory Students
- Topic: Hematology
PREFACE
- The lack of sufficient reference materials and uniformity in course syllabi has been a problem in higher institutions in Ethiopia that are engaged in training health professionals including laboratory technologists.
- The authors hope their lecture notes will be immensely useful, providing learning objectives, backgrounds for the topics in discussion, as well as study questions.
- Important terms are defined in a glossary section at the end of the text.
- Contains 22 chapters.
TABLE OF CONTENTS
- Contains the titles and page numbers for chapters ranging from Blood to Automation in Hematology.
INTRODUCTION
- The word hematology comes from the Greek words "haima" (blood) and "logos" (discourse) and is the science of blood.
- The study includes blood cells, their precursors in the bone marrow, plasma/serum components, and blood coagulation.
- The history of blood study stretches back to primitive man.
- Early approaches were descriptive, but microscopic examination led to deeper understanding.
- Modern technology allows for automated and molecular biological analysis of cells and proteins.
CHAPTER ONE: BLOOD
- Learning Objectives: Explain composition, describe function, describe blood cell formation, indicate sites of hemopoiesis.
- Composition of blood: blood plasma (about 55%) and formed elements (about 45%). Plasma is mainly water (91.5%) with blood proteins, various solutes, nutrients, etc.
- Formed elements: red blood cells (erythrocytes); white blood cells (leucocytes); and platelets (thrombocytes).
- Blood plasma: mainly water (about 91.5%); proteins (albumins, globulins, fibrinogen) compose about 8.5%; and electrolytes, gases, and waste products.
- Formed elements (cells): their numbers and constant levels in health.
OTHER CHAPTERS
- The remaining chapters (2-22) cover detailed topics in hematology, including discussions of blood collection, anticoagulants, blood smear preparation, staining methods, red blood cell indices, erythrocyte sedimentation rate, osmotic fragility, bone marrow examination, and other relevant hematological subjects.
- Detailed information and diagrams are included for each chapter.
REVIEW QUESTIONS
- (For each chapter) Questions are designed to assess understanding of the related chapter's topic.
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