Human Blood Physiology Quiz

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Questions and Answers

What percentage of whole blood is constituted by formed elements?

  • 55%
  • 45% (correct)
  • 8.5%
  • 7%

What is the normal pH range of human arterial blood?

  • 7.45-7.55
  • 7.35-7.45 (correct)
  • 7.40-7.50
  • 7.25-7.35

Which plasma protein constitutes the largest percentage of plasma proteins?

  • Globulins
  • Albumins (correct)
  • Immunoglobulins
  • Fibrinogen

What shape do mature red blood cells take?

<p>Biconcave disc (A)</p> Signup and view all the answers

Which of the following statements is true about blood plasma?

<p>It appears yellow in color. (B)</p> Signup and view all the answers

How much blood volume does an average adult human have?

<p>5 liters (C)</p> Signup and view all the answers

What role do plasma proteins play in the body?

<p>They maintain proper blood osmotic pressure. (B)</p> Signup and view all the answers

Where are red blood cells primarily formed in adults?

<p>Bone marrow (A)</p> Signup and view all the answers

What is the primary focus of the lecture notes authored by Yared Alemu and others?

<p>Training health professionals, particularly laboratory technologists (B)</p> Signup and view all the answers

Which organizations collaborated in the production of the lecture notes?

<p>Ethiopia Public Health Training Initiative and The Carter Center (D)</p> Signup and view all the answers

How many chapters are included in the lecture notes?

<p>22 (C)</p> Signup and view all the answers

What type of permissions is granted for printing and photocopying the lecture notes?

<p>Free use for educational, not-for-profit purposes (A)</p> Signup and view all the answers

What is the intended audience for these lecture notes?

<p>Practicing health care workers and students (A)</p> Signup and view all the answers

What do the chapters in the lecture notes begin with?

<p>Specific learning objectives (C)</p> Signup and view all the answers

What problem are the authors trying to address with these lecture notes?

<p>Insufficient reference materials and lack of uniformity in course syllabi (B)</p> Signup and view all the answers

What is prohibited under the guidelines for using these lecture notes?

<p>Commercial distribution or selling (A)</p> Signup and view all the answers

Where does hemopoiesis first establish during fetal life?

<p>Yolk sac mesenchyme (B)</p> Signup and view all the answers

At what point does bone marrow completely take over hemopoiesis from the liver?

<p>At term (A)</p> Signup and view all the answers

Which bones are primarily involved in hemopoiesis from infancy to adulthood?

<p>Sternum, ribs, and pelvis (C)</p> Signup and view all the answers

What role do extracellular matrix components like fibronectin and collagen play in hemopoiesis?

<p>They provide a supportive environment for stem cells. (D)</p> Signup and view all the answers

What stimulates the proliferation of erythrocyte precursors?

<p>Erythropoietin (C)</p> Signup and view all the answers

Which cells are responsible for producing various cytokines that regulate hematopoiesis?

<p>Red bone marrow cells, leukocytes, macrophages, and fibroblasts (D)</p> Signup and view all the answers

How does the body respond to increased demands for blood cells?

<p>By increasing cell production (A)</p> Signup and view all the answers

What separates the extravascular marrow compartment from the intravascular compartment?

<p>A single layer of endothelial cells (A)</p> Signup and view all the answers

What is the typical size of a mature basophil?

<p>10-12µm (B)</p> Signup and view all the answers

What characteristic helps to identify a monoblast under histochemical criteria?

<p>Delicate blue to purple stippling chromatin (A)</p> Signup and view all the answers

Which of the following is true regarding eosinophils in deficiency states?

<p>Eosinophils with more than two lobes are indicative of vitamin B12 deficiency. (A)</p> Signup and view all the answers

What is a distinguishing feature of the promonocyte?

<p>It is the only cell capable of mitotic division among monocytic cells. (A)</p> Signup and view all the answers

How would you describe the cytoplasm of mature monocytes?

<p>Sparse and gray-blue with fine azurophilic granules. (D)</p> Signup and view all the answers

What type of nuclear shape can be observed in mature eosinophils?

<p>Complex multilobed (C)</p> Signup and view all the answers

What is the nuclear/cytoplasmic ratio in a promonocyte?

<p>7:1 (C)</p> Signup and view all the answers

Which feature differentiates basophils from eosinophils in terms of their granules?

<p>Eosinophil granules are primarily orange-pink. (C)</p> Signup and view all the answers

What is the main purpose of a thin blood film in hematology?

<p>To allow for clearer visualization of blood cell morphology (D)</p> Signup and view all the answers

Which statement best describes the preparation technique for a thin blood film?

<p>Spreading a small drop of blood until small prints are visible through the smear. (B)</p> Signup and view all the answers

What is a potential effect of using a blood sample that has been kept at room temperature for an extended period on blood cell morphology?

<p>It can cause distortion of cell shapes and alterations in staining properties. (D)</p> Signup and view all the answers

Which dye mixture was developed by Romanowsky for staining blood cells?

<p>Old methylene blue mixed with eosin and dissolved in methyl alcohol (A)</p> Signup and view all the answers

What color does eosin stain in blood films due to its acidic nature?

<p>Red, due to interaction with basic components of cells (D)</p> Signup and view all the answers

What characterizes the staining properties of methylene blue in Romanowsky stains?

<p>It stains acidic parts of the cell, such as nucleic acids. (B)</p> Signup and view all the answers

Which of the following is NOT a desirable quality of a thin blood film?

<p>A thickness suited for visualizing all cell types clearly (B)</p> Signup and view all the answers

What problem may arise during the staining of blood films?

<p>Using insufficient dye concentration (D)</p> Signup and view all the answers

What color are the nuclei of leucocytes when viewed in a Romanowsky-stained blood film?

<p>Blue to purple (A)</p> Signup and view all the answers

Which granules are distinctly red orange in color in a Romanowsky-stained blood film?

<p>Eosinophilic granules (A)</p> Signup and view all the answers

What is the diluent used for Giemsa stain in buffered distilled water?

<p>pH 7.1-7.2 (A)</p> Signup and view all the answers

During the Jenner-Giemsa staining method, how long should the films be immersed in methanol for fixation?

<p>10-20 minutes (C)</p> Signup and view all the answers

Which component of the blood film has a sky blue cytoplasm and red purple chromatin when infected with malaria?

<p>Malaria parasites (C)</p> Signup and view all the answers

What is the main purpose of panoptic staining?

<p>To improve the visualization of cytoplasmic granules and nucleoli (D)</p> Signup and view all the answers

What color are platelet granules in a Romanowsky-stained blood film?

<p>Violet (D)</p> Signup and view all the answers

What should be done after staining with Giemsa stain in the preparation process?

<p>Wash with distilled water and air dry (A)</p> Signup and view all the answers

Flashcards

Lack of Reference Materials

The shortage of adequate and consistent materials for learning, particularly in medical laboratory technology courses in Ethiopia.

Non-Uniform Course Syllabi

The lack of uniform course content across different institutions, resulting in inconsistent learning experiences for students.

Purpose of Lecture Notes

The goal of the lecture notes is to address the existing problems of insufficient resources and inconsistent learning materials.

Target Audience

These lecture notes are designed to be used by medical laboratory technologists during their training and while they are working in a lab environment.

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Structure of Lecture Notes

The lecture notes consist of 22 individual chapters, each focusing on a specific topic.

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Learning Objectives

Each chapter starts with a list of specific learning objectives to guide students through the topic.

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Background Information

Each chapter includes a background section that provides context and introductory information about the topic.

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Chapter Review Questions

At the end of each chapter, questions are provided for students to assess their comprehension of the material.

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Blood Plasma

The liquid component of blood, primarily composed of water and solutes, including proteins, waste products, nutrients, and electrolytes.

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Formed elements

The main components of blood, excluding plasma, encompassing red blood cells, white blood cells, and platelets.

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Erythrocytes

Red blood cells, responsible for transporting oxygen throughout the body.

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Hemoglobin

A protein found in red blood cells that binds to oxygen, enabling its transport throughout the body.

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Leukocytes

White blood cells, responsible for defending the body against infections and foreign invaders.

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Platelets (Thrombocytes)

Small, cell-like fragments involved in blood clotting.

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Hematopoiesis

The process of blood cell formation, primarily occurring in the bone marrow.

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pH of blood

A measure of the alkalinity or acidity of a solution. In human blood, it is slightly alkaline, typically around 7.4.

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Bone Marrow

The primary site of blood cell production in adults. It's located in the central skeleton, like the sternum and ribs.

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Hematopoietic Transition

The process of blood cell production shifting from the yolk sac, liver, and spleen to the bone marrow.

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Reticulin Framework

The fine network of connective tissue that supports the bone marrow cells.

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Hematopoietic Growth Factors

Special proteins that stimulate the growth and differentiation of specific blood cell types.

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Erythropoietin (EPO)

A hormone produced mainly by the kidneys that stimulates the production of red blood cells.

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Thrombopoietin

A hormone that stimulates the production of platelets, which are essential for blood clotting.

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Cytokines

Small proteins produced by various cells that regulate the production of different blood cell types.

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Eosinophil

A type of white blood cell characterized by its bilobed nucleus and orange-pink granules in the cytoplasm.

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Basophil

A type of white blood cell with a distinctive S-shaped nucleus and dark blue-purple granules in the cytoplasm.

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Promonocyte

The earliest recognizable stage in the monocyte lineage, capable of further division.

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Monocyte

A type of white blood cell with a large, kidney-shaped nucleus and fine granules in the cytoplasm.

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Macrophage

The largest type of white blood cell, responsible for phagocytosis and immune response.

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Monoblast

A precursor to mature monocyte cells, potentially differentiating from myeloblasts.

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Auer bodies

Abnormal granules found in the cytoplasm of some leukemic cells, often associated with acute myeloid leukemia.

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Thin blood film

A thin, evenly spread layer of blood on a microscope slide used for examining individual blood cells.

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Blood film preparation

This technique involves spreading a small drop of blood over the slide, producing a thin, even layer for microscopic viewing.

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Desirable qualities of a thin blood film

A good blood film should have a smooth, even spread, clear edges, and no air bubbles. It should also contain a gradual transition from thick to thin.

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Effect of room temperature blood sample

Changes in cell morphology may occur. Red blood cells can shrink or become crenated, while white blood cells may lose their characteristic features.

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Romanowsky staining

A staining method used to enhance the visibility of different blood cell components for microscopic examination.

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Principle of staining

The principle of staining relies on the attraction of acidic and basic dyes to oppositely charged cellular components. Acidic dyes stain basic components, while basic dyes stain acidic components.

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Thick blood film

A technique used to examine blood parasites like malaria, where a thicker layer of blood is stained to increase the chances of detecting parasites.

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Panoptic stains

These stains, such as Giemsa and Wright, combine multiple dyes to achieve a comprehensive staining of blood cells for detailed microscopic analysis.

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Red Blood Cells (RBCs)

Round, biconcave cells that lack nuclei. They appear pink with a central pale area when stained with Romanowsky stain. These cells transport oxygen throughout the body.

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Leukocyte Nuclei

The nucleus of white blood cells is stained blue to purple by Romanowsky stain. This staining allows for the identification of the different types of white blood cells based on their nuclear shape and characteristics.

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Neutrophilic Granules

These granules, found in neutrophils, appear tan when stained with Romanowsky stain. These granules contain enzymes that help combat infections.

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Eosinophilic Granules

These granules, found in eosinophils, appear bright red-orange after Romanowsky staining. They play a role in fighting parasites and allergic reactions.

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Basophilic Granules

Appear dark blue after Romanowsky staining. These granules, found in basophils, contain histamine and heparin, which are involved in inflammation and allergic responses.

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Platelets

Small, violet-stained granules found in blood smears. They are essential for blood clotting, forming a plug at the site of injury to stop bleeding.

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Study Notes

LECTURE NOTES

  • For Medical Laboratory Students
  • Topic: Hematology

PREFACE

  • The lack of sufficient reference materials and uniformity in course syllabi has been a problem in higher institutions in Ethiopia that are engaged in training health professionals including laboratory technologists.
  • The authors hope their lecture notes will be immensely useful, providing learning objectives, backgrounds for the topics in discussion, as well as study questions.
  • Important terms are defined in a glossary section at the end of the text.
  • Contains 22 chapters.

TABLE OF CONTENTS

  • Contains the titles and page numbers for chapters ranging from Blood to Automation in Hematology.

INTRODUCTION

  • The word hematology comes from the Greek words "haima" (blood) and "logos" (discourse) and is the science of blood.
  • The study includes blood cells, their precursors in the bone marrow, plasma/serum components, and blood coagulation.
  • The history of blood study stretches back to primitive man.
  • Early approaches were descriptive, but microscopic examination led to deeper understanding.
  • Modern technology allows for automated and molecular biological analysis of cells and proteins.

CHAPTER ONE: BLOOD

  • Learning Objectives: Explain composition, describe function, describe blood cell formation, indicate sites of hemopoiesis.
  • Composition of blood: blood plasma (about 55%) and formed elements (about 45%). Plasma is mainly water (91.5%) with blood proteins, various solutes, nutrients, etc.
  • Formed elements: red blood cells (erythrocytes); white blood cells (leucocytes); and platelets (thrombocytes).
  • Blood plasma: mainly water (about 91.5%); proteins (albumins, globulins, fibrinogen) compose about 8.5%; and electrolytes, gases, and waste products.
  • Formed elements (cells): their numbers and constant levels in health.

OTHER CHAPTERS

  • The remaining chapters (2-22) cover detailed topics in hematology, including discussions of blood collection, anticoagulants, blood smear preparation, staining methods, red blood cell indices, erythrocyte sedimentation rate, osmotic fragility, bone marrow examination, and other relevant hematological subjects.
  • Detailed information and diagrams are included for each chapter.

REVIEW QUESTIONS

  • (For each chapter) Questions are designed to assess understanding of the related chapter's topic.

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