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History and Tools of DNA Manipulation
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History and Tools of DNA Manipulation

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Questions and Answers

Which of the following statements about genetic engineering is NOT true?

  • It allows for the direct alteration of an organism's genetics to achieve desirable traits.
  • It involves the use of recombinant DNA technology.
  • It is the process by which a DNA sequence is manipulated in vivo. (correct)
  • It can create transgenic organisms by introducing DNA from a different species.
  • What was the groundbreaking achievement demonstrated by Lydia Villa-Komaroff and Walter Gilbert's group in 1978?

  • They developed the first transgenic animal model.
  • They created the first genetically modified plant.
  • They were the first to show that a transgenic bacterial strain could produce human insulin. (correct)
  • They discovered the structure of DNA.
  • Which of the following is NOT a mechanism by which prokaryotes can acquire foreign DNA through horizontal gene transfer?

  • Conjugation (mating with other cells)
  • Transformation (taking up DNA from the environment)
  • Transduction (viral infection)
  • Transcription (synthesis of RNA from DNA) (correct)
  • What is the role of the thermal cycler in the polymerase chain reaction (PCR)?

    <p>It automates the heating and cooling cycles required for DNA amplification.</p> Signup and view all the answers

    Which of the following statements about recombinant DNA technology is correct?

    <p>It creates new combinations of genetic material by combining DNA from different sources.</p> Signup and view all the answers

    What is the primary purpose of using restriction enzymes in genetic engineering?

    <p>To cleave DNA molecules at specific recognition sequences.</p> Signup and view all the answers

    Which of the following is NOT a product that can be synthesized by transgenic microorganisms?

    <p>Antibiotics</p> Signup and view all the answers

    What is the primary advantage of using thermophilic bacteria in the polymerase chain reaction (PCR)?

    <p>Their DNA polymerase enzymes are more stable at high temperatures.</p> Signup and view all the answers

    Which of the following statements about plasmids is correct?

    <p>They are small, circular DNA molecules found in prokaryotes.</p> Signup and view all the answers

    What is the primary purpose of using transgenic host organisms in genetic engineering?

    <p>To produce and secrete desired proteins or compounds.</p> Signup and view all the answers

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