12 Questions
Why is it recommended to suspend human brains by a cord under the Circle of Willis?
To avoid flattening of the brain
What may happen if eyes are dissected before they are fixed?
Immediate tissue collapse and wrinkling
Why should water not be used for glycogen-containing tissues?
Glycogen is soluble in water
What is the purpose of injecting formal-alcohol before immersing an organ in the fixative?
To facilitate the penetration of the fixative into tough tissues
Why is it recommended to stretch fresh muscle biopsy material before fixing it?
To avoid staining artifacts
What is a suitable method for fixing hard tissues like hyperkeratotic skin or fingernails?
Immersion in 4% aqueous phenol solution for 1-3 days
Why should tissues containing mucus be fixed slowly?
To prevent the mucus from interfering with the fixative penetration.
Why should fatty tissues be cut into thin sections and fixed longer?
To ensure the fixative penetrates adequately due to the tissue's composition.
What is the purpose of washing away excess mucus with normal saline solution?
To allow better fixation of the tissue.
Why is it recommended to flush out blood from tissues like blood vessels and spleen before fixation?
To ensure proper fixation by removing blood which may interfere with fixative penetration.
Why should specimens be gently agitated during their first few minutes in fixative?
To facilitate proper penetration of the fixative into the specimen.
Why is it important to use fresh and high-quality reagents when preparing fixatives?
To ensure stability and effectiveness of the fixative.
Test your knowledge on fixation techniques for brains and eyes in histology. Learn about preventing tissue collapse, optimal fixation times, and artifact formation.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
