Histology Quiz: Brain and Eye Fixation Techniques
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Questions and Answers

Why is it recommended to suspend human brains by a cord under the Circle of Willis?

  • To avoid flattening of the brain (correct)
  • To accelerate the fixation process
  • To prevent immediate tissue collapse and wrinkling
  • To ensure rapid access of the fixative to all parts of the brain

What may happen if eyes are dissected before they are fixed?

  • Rapid access of the fixative to all parts of the eye
  • Loss of blood content
  • Immediate tissue collapse and wrinkling (correct)
  • Formation of ice crystal artifacts

Why should water not be used for glycogen-containing tissues?

  • Glycogen is soluble in water (correct)
  • Water may cause rapid dehydration of the tissues
  • Water may cause fixation artifacts
  • Water may lead to immediate tissue collapse

What is the purpose of injecting formal-alcohol before immersing an organ in the fixative?

<p>To facilitate the penetration of the fixative into tough tissues (D)</p> Signup and view all the answers

Why is it recommended to stretch fresh muscle biopsy material before fixing it?

<p>To avoid staining artifacts (A)</p> Signup and view all the answers

What is a suitable method for fixing hard tissues like hyperkeratotic skin or fingernails?

<p>Immersion in 4% aqueous phenol solution for 1-3 days (D)</p> Signup and view all the answers

Why should tissues containing mucus be fixed slowly?

<p>To prevent the mucus from interfering with the fixative penetration. (C)</p> Signup and view all the answers

Why should fatty tissues be cut into thin sections and fixed longer?

<p>To ensure the fixative penetrates adequately due to the tissue's composition. (A)</p> Signup and view all the answers

What is the purpose of washing away excess mucus with normal saline solution?

<p>To allow better fixation of the tissue. (C)</p> Signup and view all the answers

Why is it recommended to flush out blood from tissues like blood vessels and spleen before fixation?

<p>To ensure proper fixation by removing blood which may interfere with fixative penetration. (C)</p> Signup and view all the answers

Why should specimens be gently agitated during their first few minutes in fixative?

<p>To facilitate proper penetration of the fixative into the specimen. (C)</p> Signup and view all the answers

Why is it important to use fresh and high-quality reagents when preparing fixatives?

<p>To ensure stability and effectiveness of the fixative. (D)</p> Signup and view all the answers

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