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Histology Quiz: Brain and Eye Fixation Techniques

12 Questions

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Histology Quiz: Brain and Eye Fixation Techniques

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Questions and Answers

Why is it recommended to suspend human brains by a cord under the Circle of Willis?

To avoid flattening of the brain (correct)

To accelerate the fixation process

To prevent immediate tissue collapse and wrinkling

To ensure rapid access of the fixative to all parts of the brain

What may happen if eyes are dissected before they are fixed?

Rapid access of the fixative to all parts of the eye

Loss of blood content

Immediate tissue collapse and wrinkling (correct)

Formation of ice crystal artifacts

Why should water not be used for glycogen-containing tissues?

Glycogen is soluble in water (correct)

Water may cause rapid dehydration of the tissues

Water may cause fixation artifacts

Water may lead to immediate tissue collapse

What is the purpose of injecting formal-alcohol before immersing an organ in the fixative?

To facilitate the penetration of the fixative into tough tissues Signup and view all the answers

Why is it recommended to stretch fresh muscle biopsy material before fixing it?

To avoid staining artifacts Signup and view all the answers

What is a suitable method for fixing hard tissues like hyperkeratotic skin or fingernails?

Immersion in 4% aqueous phenol solution for 1-3 days Signup and view all the answers

Why should tissues containing mucus be fixed slowly?

To prevent the mucus from interfering with the fixative penetration. Signup and view all the answers

Why should fatty tissues be cut into thin sections and fixed longer?

To ensure the fixative penetrates adequately due to the tissue's composition. Signup and view all the answers

What is the purpose of washing away excess mucus with normal saline solution?

To allow better fixation of the tissue. Signup and view all the answers

Why is it recommended to flush out blood from tissues like blood vessels and spleen before fixation?

To ensure proper fixation by removing blood which may interfere with fixative penetration. Signup and view all the answers

Why should specimens be gently agitated during their first few minutes in fixative?

To facilitate proper penetration of the fixative into the specimen. Signup and view all the answers

Why is it important to use fresh and high-quality reagents when preparing fixatives?

To ensure stability and effectiveness of the fixative. Signup and view all the answers