15 Questions
What is the main advantage of Paraplast over normal paraffin wax?
Greater elasticity
Why should Paraplast not be used for thin-walled structures?
Prevents complete expansion of the specimen
How many changes in wax should the tissue be submitted to during impregnation with Bioloid?
3 changes
What causes crumbling of the sections during cutting?
Residual clearing agent with the wax
Why does a thicker tissue require longer time for wax penetration?
More volume of tissue and clearing agent
Which type of mould consists of square-shaped brass or metal plates in a series of interlocking plates?
Compound embedding units
What is the main purpose of embedding tissue in melted paraffin?
To provide a firm medium for keeping intact all parts of the tissue when sections are cut
What is the advantage of using a vacuum embedding oven over a normal paraffin oven?
It reduces the need for multiple changes of paraffin wax
Which clearing agent is easiest to remove, requiring only one change of wax for impregnation?
Xylene
How many changes of wax are needed for chloroform and carbon tetrachloride during impregnation?
2-3 changes
What temperature is the paraffin wax heated to before pouring into the mould?
2-3°C above the melting point
Why is it important to introduce the tissue with prewarmed forceps into the semi-solid wax?
To prevent the wax from sticking to the tissue
What is the purpose of submerging the whole block with mould in cold water at 20°C?
To prevent the block from cracking
Why should the tissue surface towards the mould base be lightly trimmed with a scalpel after the blocks are set hard?
To expose the tissue
From which surface should the sections be cut after the blocks are set hard?
Surface towards the mould base
Test your knowledge about histology embedding media with this quiz. Explore questions about Paraplast, Bioloid, and techniques for specimen preparation.
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