Histology Embedding Media Quiz

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Questions and Answers

What is the main advantage of Paraplast over normal paraffin wax?

  • Greater elasticity (correct)
  • Higher melting point
  • Lower cost
  • Better color retention

Why should Paraplast not be used for thin-walled structures?

  • Improves color retention
  • Allows for easier sectioning
  • Reduces the need for clearing agent
  • Prevents complete expansion of the specimen (correct)

How many changes in wax should the tissue be submitted to during impregnation with Bioloid?

  • 5 changes
  • 1 change
  • No changes
  • 3 changes (correct)

What causes crumbling of the sections during cutting?

<p>Residual clearing agent with the wax (D)</p> Signup and view all the answers

Why does a thicker tissue require longer time for wax penetration?

<p>More volume of tissue and clearing agent (D)</p> Signup and view all the answers

Which type of mould consists of square-shaped brass or metal plates in a series of interlocking plates?

<p>Compound embedding units (A)</p> Signup and view all the answers

What is the main purpose of embedding tissue in melted paraffin?

<p>To provide a firm medium for keeping intact all parts of the tissue when sections are cut (A)</p> Signup and view all the answers

What is the advantage of using a vacuum embedding oven over a normal paraffin oven?

<p>It reduces the need for multiple changes of paraffin wax (C)</p> Signup and view all the answers

Which clearing agent is easiest to remove, requiring only one change of wax for impregnation?

<p>Xylene (A)</p> Signup and view all the answers

How many changes of wax are needed for chloroform and carbon tetrachloride during impregnation?

<p>2-3 changes (B)</p> Signup and view all the answers

What temperature is the paraffin wax heated to before pouring into the mould?

<p>2-3°C above the melting point (C)</p> Signup and view all the answers

Why is it important to introduce the tissue with prewarmed forceps into the semi-solid wax?

<p>To prevent the wax from sticking to the tissue (D)</p> Signup and view all the answers

What is the purpose of submerging the whole block with mould in cold water at 20°C?

<p>To prevent the block from cracking (D)</p> Signup and view all the answers

Why should the tissue surface towards the mould base be lightly trimmed with a scalpel after the blocks are set hard?

<p>To expose the tissue (D)</p> Signup and view all the answers

From which surface should the sections be cut after the blocks are set hard?

<p>Surface towards the mould base (A)</p> Signup and view all the answers

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