Histology Dehydrating Agents Quiz
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Questions and Answers

Which dehydrating agent has a rapid action but produces shrinkage, distortion, and subsequent brittleness to the tissue?

  • Isopropyl alcohol
  • Water
  • Dioxane
  • Acetone (correct)
  • Which dehydrating agent dehydrates and clears at the same time, and is miscible with paraffin and water/alcohol?

  • Ethanol
  • Acetone
  • Dioxane (correct)
  • Isopropyl alcohol
  • Which dehydrating agent is miscible with water and other organic solvents, does not harden tissue like alcohol, and is expensive?

  • Isopropyl alcohol (correct)
  • Formaldehyde
  • Dioxane
  • Acetone
  • Which dehydrating agent is toxic to humans and is more expensive than alcohol?

    <p>Dioxane</p> Signup and view all the answers

    What is the purpose of a clearing agent in tissue treatment?

    <p>To make the tissue translucent</p> Signup and view all the answers

    Which clearing agent has a slower action but causes less brittleness to the tissue, allowing it to be left overnight?

    <p>Chloroform</p> Signup and view all the answers

    Which clearing agent is good for delicate tissues as it has the least hardening effect and is very slow in action?

    <p>Cedar wood oil</p> Signup and view all the answers

    In which clearing agent may the tissue be left overnight, and in an automatic tissue processor three changes of one hour each are usually satisfactory?

    <p>Chloroform</p> Signup and view all the answers

    Which clearing agent has a rapid action, with biopsy specimens of 3-4 mm thickness being cleared in 2-4 hours?

    <p>Toluene</p> Signup and view all the answers

    Which clearing agent is similar in properties to xylene but is cheaper?

    <p>Carbon tetrachloride</p> Signup and view all the answers

    What is the purpose of impregnation with paraffin wax?

    <p>To support the tissue during cutting</p> Signup and view all the answers

    What should paraffin wax be free from during use?

    <p>Dust, grit, and other foreign matter</p> Signup and view all the answers

    Why is frequent temperature check of paraffin baths required?

    <p>To prevent tissue shrinkage and hardening</p> Signup and view all the answers

    What does overheating paraffin wax cause?

    <p>Tissue shrinkage</p> Signup and view all the answers

    What are the properties of paraffin wax that make it advantageous for impregnation?

    <p>Easy preparation of large number of tissue blocks</p> Signup and view all the answers

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