Histology Dehydrating Agents Quiz

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Questions and Answers

Which dehydrating agent has a rapid action but produces shrinkage, distortion, and subsequent brittleness to the tissue?

  • Isopropyl alcohol
  • Water
  • Dioxane
  • Acetone (correct)

Which dehydrating agent dehydrates and clears at the same time, and is miscible with paraffin and water/alcohol?

  • Ethanol
  • Acetone
  • Dioxane (correct)
  • Isopropyl alcohol

Which dehydrating agent is miscible with water and other organic solvents, does not harden tissue like alcohol, and is expensive?

  • Isopropyl alcohol (correct)
  • Formaldehyde
  • Dioxane
  • Acetone

Which dehydrating agent is toxic to humans and is more expensive than alcohol?

<p>Dioxane (A)</p> Signup and view all the answers

What is the purpose of a clearing agent in tissue treatment?

<p>To make the tissue translucent (C)</p> Signup and view all the answers

Which clearing agent has a slower action but causes less brittleness to the tissue, allowing it to be left overnight?

<p>Chloroform (B)</p> Signup and view all the answers

Which clearing agent is good for delicate tissues as it has the least hardening effect and is very slow in action?

<p>Cedar wood oil (D)</p> Signup and view all the answers

In which clearing agent may the tissue be left overnight, and in an automatic tissue processor three changes of one hour each are usually satisfactory?

<p>Chloroform (B)</p> Signup and view all the answers

Which clearing agent has a rapid action, with biopsy specimens of 3-4 mm thickness being cleared in 2-4 hours?

<p>Toluene (C)</p> Signup and view all the answers

Which clearing agent is similar in properties to xylene but is cheaper?

<p>Carbon tetrachloride (D)</p> Signup and view all the answers

What is the purpose of impregnation with paraffin wax?

<p>To support the tissue during cutting (B)</p> Signup and view all the answers

What should paraffin wax be free from during use?

<p>Dust, grit, and other foreign matter (A)</p> Signup and view all the answers

Why is frequent temperature check of paraffin baths required?

<p>To prevent tissue shrinkage and hardening (C)</p> Signup and view all the answers

What does overheating paraffin wax cause?

<p>Tissue shrinkage (C)</p> Signup and view all the answers

What are the properties of paraffin wax that make it advantageous for impregnation?

<p>Easy preparation of large number of tissue blocks (C)</p> Signup and view all the answers

Flashcards

Acetone

A dehydrating agent with rapid action that causes shrinkage and brittleness.

Dioxane

A dehydrating and clearing agent, miscible with paraffin and water/alcohol.

Isopropyl alcohol

A dehydrating agent miscible with water/organic solvents, doesn't harden tissue like alcohol, but is expensive.

Dioxane

A toxic dehydrating agent, more expensive than alcohol.

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Purpose of clearing agent

To make the tissue translucent.

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Chloroform

A clearing agent with a slower action that causes less brittleness, suitable for overnight use.

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Cedar wood oil

A clearing agent with the least hardening effect, good for delicate tissues, but is very slow.

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Chloroform (clearing)

Clearing agent suitable for overnight use; 3 changes of one hour each in an automatic tissue processor are usually satisfactory.

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Toluene

A clearing agent with rapid action, clearing biopsy specimens of 3-4 mm thickness in 2-4 hours.

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Carbon tetrachloride

A clearing agent similar in properties to xylene but cheaper.

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Purpose of paraffin wax impregnation

To support the tissue during cutting.

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Paraffin wax purity

Free from dust, grit, and other foreign matter.

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Temperature check of paraffin baths

To prevent tissue shrinkage and hardening.

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Overheating paraffin wax

Tissue shrinkage.

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Advantage of paraffin wax

Easy preparation of a large number of tissue blocks.

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