Podcast
Questions and Answers
Who is the professor of the course?
Who is the professor of the course?
- Hanyang Hu
- Lin Wang (correct)
- Rong Liu
- Yanwu Wang
When is the lecture of the course?
When is the lecture of the course?
- Tuesday, 9:50 – 12:15 AM (correct)
- Thursday, 9:50 – 12:15 AM
- Friday, 9:50 – 12:15 AM
- Monday, 9:50 – 12:15 AM
What is the topic of the 5th lecture?
What is the topic of the 5th lecture?
- Nervous tissue (correct)
- Circulatory System
- Endocrine system
- Immune system
What is the topic of Lab 3?
What is the topic of Lab 3?
What is the goal of the course?
What is the goal of the course?
How many primary tissues of the human body will you learn in this course?
How many primary tissues of the human body will you learn in this course?
What is one of the course formative grading criteria?
What is one of the course formative grading criteria?
When is the last lab of the course?
When is the last lab of the course?
What is the smallest structural and functional unit in the human body?
What is the smallest structural and functional unit in the human body?
What is the primary focus of Embryology?
What is the primary focus of Embryology?
What are the four types of fundamental tissues?
What are the four types of fundamental tissues?
Why is it important to learn histology?
Why is it important to learn histology?
What is the purpose of fixation in paraffin section preparation?
What is the purpose of fixation in paraffin section preparation?
What is the purpose of studying histology?
What is the purpose of studying histology?
What is the advantage of frozen section preparation?
What is the advantage of frozen section preparation?
What is the importance of histology in clinic sciences?
What is the importance of histology in clinic sciences?
What is the purpose of staining in histology?
What is the purpose of staining in histology?
What is one of the investigative methods of histology?
What is one of the investigative methods of histology?
What is the definition of histology?
What is the definition of histology?
What is hematoxylin?
What is hematoxylin?
What is metachromasia?
What is metachromasia?
What type of microscope uses a beam of electrons instead of visible light?
What type of microscope uses a beam of electrons instead of visible light?
What is the purpose of dehydration in paraffin section preparation?
What is the purpose of dehydration in paraffin section preparation?
What is the commonest method used in histology for tissue preparation?
What is the commonest method used in histology for tissue preparation?
What is the main difference between the preparation of sections for LM and EM?
What is the main difference between the preparation of sections for LM and EM?
What is the resolution of the Electron Microscope (EM)?
What is the resolution of the Electron Microscope (EM)?
What is the main purpose of Histochemistry and Cytochemistry?
What is the main purpose of Histochemistry and Cytochemistry?
What is the product of a chemical reaction in Histochemistry and Cytochemistry?
What is the product of a chemical reaction in Histochemistry and Cytochemistry?
What is the purpose of PAS reaction in Histochemistry and Cytochemistry?
What is the purpose of PAS reaction in Histochemistry and Cytochemistry?
What is the purpose of Cell and Tissue Culture?
What is the purpose of Cell and Tissue Culture?
What is the resolution of the Scanning Electron Microscope (SEM)?
What is the resolution of the Scanning Electron Microscope (SEM)?
What is the principle of Immunocytochemistry?
What is the principle of Immunocytochemistry?
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Study Notes
Course Information
- Instructor: Lin Wang (汪琳)
- Lectures: Tuesday, 9:50 – 12:15 AM, Room 4-121
- Labs: Friday, 9:50 -11:25 AM, Room 4-121
Course Objectives
- Identify and name the four primary tissues of the human body
- Explain how the form and function of these tissues are related
- Identify and name the basic tissue constituents of the major organs of the human being
- Explain how the form and function of these organs are related
Definition of Histology
- A science that studies the normal micro-structure and its related function in the human body
- Gross anatomy vs. micro-anatomy
Research Content
- 4 structural levels: cell, tissue, organ, system
- 4 types of fundamental tissue: epithelium, connective tissue, muscular tissue, nervous tissue
Importance of Histology
- Recognize normal tissue and cells
- Acquire basic skills for future medical careers
- Complete knowledge of human body structures from gross to microscopic
- Understand how different tissues function
- Find diseases by knowing normal tissue structures
Investigative Methods of Histology
I. Light Microscope
- Tissue preparation:
- Paraffin section preparation (most common method)
- Frozen section (fastest method)
- Smear preparations (for blood)
- Grind preparations (for bone)
- Staining:
- H-E Staining (most common staining procedure)
- Purpose: to make tissue section pigment for observation
- Hematoxylin: basic dye, purple-blue
- Eosin: acid dye, pink color
- Basophilic: components bonded by basic dye (H)
- Acidophilic: components bonded by acidic dye (E)
- Neutrophilic: does not stain with both basic and acid dyes
II. Electron Microscopy
- Transmission Electron Microscope (TEM)
- Section preparation: similar to LM, but with plastic (Epon or Araldite) and ultrathin sections (50-70nm)
- Heavy metal salts (lead citrate or uranyl acetate) instead of HE
- Resolution: 0.2nm
- Scanning Electron Microscope (SEM)
- Shows 3D surface architecture of cells and tissues
- Resolution: 5nm
III. Histochemistry & Cytochemistry
- Reveals chemical composition in situ with chemical, biochemical methods
- Fixation: preserves both structure and chemical composition of cells and tissues
- Specific chemical-identifying reaction carried out
- Example: PAS (Periodic Acid Schiff) reaction for manifesting polysaccharide and proteoglycan
IV. Cell / Tissue Culture
- Technique for studying living cells
- Isolated cells or fragments of tissues cultured in sterilized culture medium at appropriate temperature
- Useful for detecting the effect of various regents on living cells
V. Immunocytochemistry
- Based on antigen binds to specific antibody
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