Histological Techniques Quiz

What is the purpose of 'DEHYDRATION' in tissue processing?

Which step follows 'CLEARING' in the tissue processing procedure mentioned?

During which phase of cell division does 'interphase' occur?

In which type of cells is a true nucleus present?

What is the purpose of 'washing out' in the fixation process?

What is the cardinal sign of inflammation according to the text?

Which fixative is used for the study of pain and suffering?

What histone staining agent is mentioned in the text?

Which type of fixative is most suitable for immunoelectron microscopy according to the text?

What is the primary purpose of Adams plastic/resin mentioned in the text?

Cardinal Signs of Inflammation

PAIN is a cardinal sign of inflammation
Pathological examination involves PARABASAL CELL, which are immature cells of vaginal epithelium
Lindquistic/Rhodamine Routine Fixative is used in a 1:9 ratio
Mercuric Chloride Carnoy's Fixative is also used in histopathology techniques

Histopathology Techniques

There are 12 steps in histopathology techniques
Step 1: Numbering
Step 2: Fixation using 10% Formalin or 3% Glutaraldehyde (ideal for immunoelectron microscopy)
Step 3: Dehydration, which removes intracellular and extracellular water from the tissue
Step 4: Clearing
Step 5 & 6: Wax Impregnation and Embedding
Step 7 & 8: Blocking and Trimming
Step 9: Sectioning
Step 10: Staining, including Immunohistochemistry methods to retrieve antigens and tumor markers

Cell Biology

Eukaryotic cells have a true nucleus and membrane-bound organelles (found in human, animal, and plant cells)
Prokaryotic cells do not have a nucleus (found in bacterial cells)
Cell division involves Mitosis, which includes Interphase, Prophase, Metaphase, Anaphase, and Telophase

Cytology

Exfoliative Cytology involves preparing smears, such as the Papanicolau smear and stain (PAPS stain and procedure)
PAPS is a type of cervical screening test

Test your knowledge on various histological techniques used in pathology, such as vacuum embedding, routine fixatives, and staining methods. This quiz covers topics like cell types, fixatives, and embedding procedures.