10 Questions
What is the purpose of 'DEHYDRATION' in tissue processing?
To remove intracellular and extracellular water
Which step follows 'CLEARING' in the tissue processing procedure mentioned?
Wax impregnation and embedding
During which phase of cell division does 'interphase' occur?
Prophase
In which type of cells is a true nucleus present?
Eukaryote
What is the purpose of 'washing out' in the fixation process?
To remove artifacts and precipitates
What is the cardinal sign of inflammation according to the text?
Pain
Which fixative is used for the study of pain and suffering?
Mercuric chloride
What histone staining agent is mentioned in the text?
Honing blueing agent
Which type of fixative is most suitable for immunoelectron microscopy according to the text?
Glutaraldehyde
What is the primary purpose of Adams plastic/resin mentioned in the text?
Impregnation and embedding
Study Notes
Cardinal Signs of Inflammation
- PAIN is a cardinal sign of inflammation
- Pathological examination involves PARABASAL CELL, which are immature cells of vaginal epithelium
- Lindquistic/Rhodamine Routine Fixative is used in a 1:9 ratio
- Mercuric Chloride Carnoy's Fixative is also used in histopathology techniques
Histopathology Techniques
- There are 12 steps in histopathology techniques
- Step 1: Numbering
- Step 2: Fixation using 10% Formalin or 3% Glutaraldehyde (ideal for immunoelectron microscopy)
- Step 3: Dehydration, which removes intracellular and extracellular water from the tissue
- Step 4: Clearing
- Step 5 & 6: Wax Impregnation and Embedding
- Step 7 & 8: Blocking and Trimming
- Step 9: Sectioning
- Step 10: Staining, including Immunohistochemistry methods to retrieve antigens and tumor markers
Cell Biology
- Eukaryotic cells have a true nucleus and membrane-bound organelles (found in human, animal, and plant cells)
- Prokaryotic cells do not have a nucleus (found in bacterial cells)
- Cell division involves Mitosis, which includes Interphase, Prophase, Metaphase, Anaphase, and Telophase
Cytology
- Exfoliative Cytology involves preparing smears, such as the Papanicolau smear and stain (PAPS stain and procedure)
- PAPS is a type of cervical screening test
Test your knowledge on various histological techniques used in pathology, such as vacuum embedding, routine fixatives, and staining methods. This quiz covers topics like cell types, fixatives, and embedding procedures.
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