High Throughput Screening in Biochemical Assays Quiz

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What is the typical number of wells in a high throughput screening?

50,000 wells (correct)

10,000 wells

1 million wells

100,000 wells

What is the main reason why combining different technologies, including human input, was necessary in high throughput screening (HTS)?

To reduce the interference of pan assay interference compounds (PAINS). (correct)

To increase the success rate of identifying active compounds.

To eliminate false positives and negatives.

To overcome the issue of promiscuous inhibitors.

Why was miniaturizing the screening process considered an important milestone in HTS?

To eliminate the need for colorimetric techniques.

To enable screening of a larger number of compounds efficiently. (correct)

To increase the success rate of screening everything.

To reduce the number of inhibitors identified.

How long does a high throughput screening typically take?

Days to weeks Signup and view all the answers

What distinguishes low throughput screening from high throughput screening?

The number of compounds screened Signup and view all the answers

What was a major challenge faced in the early stages of high throughput screening (HTS) due to some compounds known as PAINS?

Non-drug like compounds appearing as hits. Signup and view all the answers

Why is it crucial to dispense reagents accurately in high throughput screening?

To ensure reliable and accurate results Signup and view all the answers

Which type of techniques were mainly adopted in high throughput screening (HTS) due to their ease of readout?

Fluorescent-based techniques. Signup and view all the answers

What is the first step in the process of high throughput screening?

Developing a primary assay Signup and view all the answers

What was one of the main reasons for the low success rate in the early stages of high throughput screening (HTS)?

Presence of pan assay interference compounds (PAINS). Signup and view all the answers

What was the purpose behind combining different technologies with human input in high throughput screening (HTS)?

To improve efficiency and smart usage of HTS. Signup and view all the answers

What is a consideration before starting multiple High Throughput Screening campaigns?

Determining confidence in the assay setup Signup and view all the answers

What is the purpose of counter screens and orthogonal screens in high throughput screening (HTS)?

To validate the selectivity of compounds Signup and view all the answers

What distinguishes fragment-based drug discovery from traditional large compound library screening?

Synthesizing smaller fragments initially Signup and view all the answers

What advantage does fragment-based drug discovery have over traditional large compound library screening?

Easier quality control of compounds Signup and view all the answers

In the context of high throughput screening, what is the primary goal when identifying fragments?

Activity against the intended target Signup and view all the answers

Why has phenotypic screening gained importance in high throughput screening (HTS) processes?

To screen larger, more drug-like compounds Signup and view all the answers

What characterizes the adaptation made to improve high throughput screening nature, as mentioned in the text?

Shifting towards fragment-based drug discovery Signup and view all the answers

High Throughput Screening in Biochemical Assays Quiz

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