18 Questions
What is the T2 virus?
A bacteriophage that infects Escherichia coli
How does a phage reproduce?
By injecting its DNA into the bacterial cell
Why did biologists conduct the Hershey-Chase experiment?
To understand the process of phage reproduction
What was the composition of the T2 phage in terms of protein and nucleic acid?
50% protein, 50% nucleic acid
How do progeny phages emerge from a cell after infection?
They escape by breaking open the cell
What role did radioactive isotopes play in the Hershey-Chase experiment?
Tracking the fate of phosphorus and sulfur in protein and DNA
What did Oswald Avery, Colin MacLeod, and Maclyn McCarty isolate and purify successfully in 1944?
DNA
Which type of enzymes were ineffective against the transforming substance identified by Avery, MacLeod, and McCarty?
Ribonuclease
What did Griffith theorize might be responsible for the transformation of the R-strain bacteria?
Polysaccharide coat of the dead bacteria
Which type of bacteria had mutated to the virulent S form according to one of Griffith's interpretations?
Type IIR
Which enzymes had an effect on eliminating the biological activity of the transforming substance?
DNAse
What did Griffith conclude was the reason behind the transformation of R-strain bacteria?
Acquiring genetic virulence of the dead S-strain bacteria
What did Griffith observe when he injected living S-strain bacteria into mice?
The mice developed pneumonia and died.
What was the result when Griffith injected R-strain bacteria into mice?
The mice lived, and no bacteria were found in their blood.
What was Griffith's expectation when he injected heat-killed S-strain bacteria into mice?
The mice would live with no bacteria in their blood.
What was Griffith's surprising observation when he infected the mice with living type IIR bacteria and heat-killed type IIIS bacteria?
The mice developed pneumonia and died.
What was significant about the type IIIS bacteria observed by Griffith in the blood of the infected mice?
They were virulent.
What conclusion did Griffith draw from the fact that the infectivity of the type IIIS bacteria was heritable through several generations?
Genetic material could be passed from bacteria to bacteria.
Test your knowledge on the Hershey–Chase experiment, also known as the Blender Experiment, which provided evidence for DNA as the genetic material. Learn about the study of the T2 virus by Alfred Hershey and Martha Chase and its implications.
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