Heparin Anticoagulation Action Quiz

What is the primary action of heparin in the context of anticoagulation?

Which of the following statements about heparin is NOT true?

What is the main purpose of the experimental method involving the saline control group?

What is the principal coagulation factor inhibited by antithrombin III, accelerated by heparin?

When collecting blood from the orbital sinus of a mouse, what is the most critical step to ensure proper blood flow?

What does the experimental group receive in the study?

What two factors primarily highlight heparin's mechanism of action when used as an anticoagulant?

What is the purpose of monitoring coagulation time in both the control and experimental groups?

Heparin Anticoagulation Action

Heparin is purified from bovine lungs or pig intestines.
Heparin is active in vitro and in vivo.
Its half-life is 1-5 hours.
Heparin's anticoagulant activity depends on antithrombin III.
Antithrombin III inhibits coagulation factors, mainly factor IIa, IXa, and Xa.
Heparin accelerates the action of antithrombin III by 1000-fold.

Experimental Procedure

Mice are weighed and randomly assigned to experimental or control groups.
0.1 ml/10g of heparin is administered intraperitoneally to the experimental group, while saline is given to the control group.
Twenty minutes later, blood is collected from the orbital sinus.
Capillary glass tubes are checked every 30 seconds until blood appears.
Coagulation time is measured and compared between the experimental and control groups.

Collecting Blood from Orbital Sinus

Position the mouse on its side on a table.
Pull the skin around the eye to expose the eye socket.
Carefully insert a capillary tube at a 45-degree angle into the eye socket, aiming toward the center
Rotate the tube while applying gentle pressure to break the vein and allow blood to enter the capillary.
Fill the capillary slightly, then cover the open end to prevent blood from spilling out.
Withdraw the capillary when enough blood has been collected, avoiding letting air enter.

Test your knowledge on the anticoagulation action of heparin, its mechanism, and experimental procedures involving heparin administration. This quiz covers the effects of heparin on coagulation and the methodology for measuring coagulation time in mice.