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Questions and Answers
What is the primary action of heparin in the context of anticoagulation?
What is the primary action of heparin in the context of anticoagulation?
- Inhibits antithrombin III
- Inhibits factor VIIIa
- Accelerates the action of antithrombin III (correct)
- Promotes coagulation factors
Which of the following statements about heparin is NOT true?
Which of the following statements about heparin is NOT true?
- Heparin enhances the activity of antithrombin III.
- Heparin is active only in vivo. (correct)
- Heparin can be derived from bovine and porcine sources.
- The half-life of heparin is between 1 to 5 hours.
What is the main purpose of the experimental method involving the saline control group?
What is the main purpose of the experimental method involving the saline control group?
- To ensure consistency in heparin dosing
- To observe the body's natural coagulation response (correct)
- To increase the anticoagulation effect of heparin
- To provide a higher dose of heparin
What is the principal coagulation factor inhibited by antithrombin III, accelerated by heparin?
What is the principal coagulation factor inhibited by antithrombin III, accelerated by heparin?
When collecting blood from the orbital sinus of a mouse, what is the most critical step to ensure proper blood flow?
When collecting blood from the orbital sinus of a mouse, what is the most critical step to ensure proper blood flow?
What does the experimental group receive in the study?
What does the experimental group receive in the study?
What two factors primarily highlight heparin's mechanism of action when used as an anticoagulant?
What two factors primarily highlight heparin's mechanism of action when used as an anticoagulant?
What is the purpose of monitoring coagulation time in both the control and experimental groups?
What is the purpose of monitoring coagulation time in both the control and experimental groups?
Flashcards
What is heparin?
What is heparin?
Heparin is a naturally occurring anticoagulant, primarily extracted from pig intestines or bovine lungs.
How does heparin work?
How does heparin work?
Heparin acts by enhancing the activity of antithrombin â…¢, a naturally occurring protein in the blood that inhibits coagulation factors.
What's special about heparin's action?
What's special about heparin's action?
Heparin's effectiveness is measured by its ability to accelerate the activity of antithrombin â…¢, which is about 1000 times faster in the presence of heparin.
Where does heparin work?
Where does heparin work?
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How do we measure heparin's effect?
How do we measure heparin's effect?
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How long does heparin last in the body?
How long does heparin last in the body?
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What is the orbital sinus?
What is the orbital sinus?
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How do we collect blood from the orbital sinus?
How do we collect blood from the orbital sinus?
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Study Notes
Heparin Anticoagulation Action
- Heparin is purified from bovine lungs or pig intestines.
- Heparin is active in vitro and in vivo.
- Its half-life is 1-5 hours.
- Heparin's anticoagulant activity depends on antithrombin III.
- Antithrombin III inhibits coagulation factors, mainly factor IIa, IXa, and Xa.
- Heparin accelerates the action of antithrombin III by 1000-fold.
Experimental Procedure
- Mice are weighed and randomly assigned to experimental or control groups.
- 0.1 ml/10g of heparin is administered intraperitoneally to the experimental group, while saline is given to the control group.
- Twenty minutes later, blood is collected from the orbital sinus.
- Capillary glass tubes are checked every 30 seconds until blood appears.
- Coagulation time is measured and compared between the experimental and control groups.
Collecting Blood from Orbital Sinus
- Position the mouse on its side on a table.
- Pull the skin around the eye to expose the eye socket.
- Carefully insert a capillary tube at a 45-degree angle into the eye socket, aiming toward the center
- Rotate the tube while applying gentle pressure to break the vein and allow blood to enter the capillary.
- Fill the capillary slightly, then cover the open end to prevent blood from spilling out.
- Withdraw the capillary when enough blood has been collected, avoiding letting air enter.
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