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Questions and Answers
What is the formula to calculate total magnification in a compound light microscope?
What is the formula to calculate total magnification in a compound light microscope?
- Objective magnification + Ocular magnification
- Objective magnification × Ocular magnification (correct)
- Objective magnification - Ocular magnification
- Objective magnification ÷ Ocular magnification
What is the maximum magnification achievable with a light microscope before there is no improvement in resolution?
What is the maximum magnification achievable with a light microscope before there is no improvement in resolution?
- ~1500X
- ~500X
- ~1000X
- ~2000X (correct)
Which type of light would be more suitable for light microscopy based on the information provided?
Which type of light would be more suitable for light microscopy based on the information provided?
- Green light
- Ultraviolet light
- Infrared light
- Blue light (correct)
What is the main purpose of staining in light microscopy?
What is the main purpose of staining in light microscopy?
Which of the following is NOT an example of a common stain used in light microscopy?
Which of the following is NOT an example of a common stain used in light microscopy?
What technique helps improve contrast in light microscopy when visualizing pigmented microorganisms?
What technique helps improve contrast in light microscopy when visualizing pigmented microorganisms?
What color do Gram-positive cells appear as after the counterstain with red safranin?
What color do Gram-positive cells appear as after the counterstain with red safranin?
What is the purpose of decolorizing with alcohol in the Gram staining technique?
What is the purpose of decolorizing with alcohol in the Gram staining technique?
How do Gram-negative bacteria appear after the Gram staining process?
How do Gram-negative bacteria appear after the Gram staining process?
What is the purpose of the iodine solution in the Gram staining technique?
What is the purpose of the iodine solution in the Gram staining technique?
Which staining technique results in Gram-positive cells being purple and Gram-negative cells being colorless?
Which staining technique results in Gram-positive cells being purple and Gram-negative cells being colorless?
Why do staining techniques kill bacteria according to the provided text?
Why do staining techniques kill bacteria according to the provided text?
What is the key factor that limits our ability to see microorganisms clearly?
What is the key factor that limits our ability to see microorganisms clearly?
Which type of microscopy visualizes specimens based on differences in contrast between the specimen and its surroundings?
Which type of microscopy visualizes specimens based on differences in contrast between the specimen and its surroundings?
What is the purpose of differential staining techniques in microbiology?
What is the purpose of differential staining techniques in microbiology?
Which type of microscopy uses specialized optics to enhance the contrast of transparent specimens?
Which type of microscopy uses specialized optics to enhance the contrast of transparent specimens?
What is the primary function of Gram staining in microbiology?
What is the primary function of Gram staining in microbiology?
Which staining technique is commonly used to distinguish between Gram-positive and Gram-negative bacteria?
Which staining technique is commonly used to distinguish between Gram-positive and Gram-negative bacteria?