Heat Fixing and Staining Process Quiz
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Questions and Answers

What is the formula to calculate total magnification in a compound light microscope?

  • Objective magnification + Ocular magnification
  • Objective magnification × Ocular magnification (correct)
  • Objective magnification - Ocular magnification
  • Objective magnification ÷ Ocular magnification

What is the maximum magnification achievable with a light microscope before there is no improvement in resolution?

  • ~1500X
  • ~500X
  • ~1000X
  • ~2000X (correct)

Which type of light would be more suitable for light microscopy based on the information provided?

  • Green light
  • Ultraviolet light
  • Infrared light
  • Blue light (correct)

What is the main purpose of staining in light microscopy?

<p>To enhance contrast (B)</p> Signup and view all the answers

Which of the following is NOT an example of a common stain used in light microscopy?

<p>Ethanol (D)</p> Signup and view all the answers

What technique helps improve contrast in light microscopy when visualizing pigmented microorganisms?

<p>Gram staining (C)</p> Signup and view all the answers

What color do Gram-positive cells appear as after the counterstain with red safranin?

<p>Pink to red (A)</p> Signup and view all the answers

What is the purpose of decolorizing with alcohol in the Gram staining technique?

<p>To differentiate between Gram-positive and Gram-negative cells (D)</p> Signup and view all the answers

How do Gram-negative bacteria appear after the Gram staining process?

<p>Pink (D)</p> Signup and view all the answers

What is the purpose of the iodine solution in the Gram staining technique?

<p>To turn all cells purple (A)</p> Signup and view all the answers

Which staining technique results in Gram-positive cells being purple and Gram-negative cells being colorless?

<p>Differential staining (B)</p> Signup and view all the answers

Why do staining techniques kill bacteria according to the provided text?

<p>The text does not specify a reason (A)</p> Signup and view all the answers

What is the key factor that limits our ability to see microorganisms clearly?

<p>Resolution (C)</p> Signup and view all the answers

Which type of microscopy visualizes specimens based on differences in contrast between the specimen and its surroundings?

<p>Bright-field (D)</p> Signup and view all the answers

What is the purpose of differential staining techniques in microbiology?

<p>To differentiate between different types of microorganisms based on their staining properties (B)</p> Signup and view all the answers

Which type of microscopy uses specialized optics to enhance the contrast of transparent specimens?

<p>Phase-contrast (D)</p> Signup and view all the answers

What is the primary function of Gram staining in microbiology?

<p>To identify differences in cell wall structure (D)</p> Signup and view all the answers

Which staining technique is commonly used to distinguish between Gram-positive and Gram-negative bacteria?

<p>Gram staining (A)</p> Signup and view all the answers

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