18 Questions
What is the formula to calculate total magnification in a compound light microscope?
Objective magnification × Ocular magnification
What is the maximum magnification achievable with a light microscope before there is no improvement in resolution?
~2000X
Which type of light would be more suitable for light microscopy based on the information provided?
Blue light
What is the main purpose of staining in light microscopy?
To enhance contrast
Which of the following is NOT an example of a common stain used in light microscopy?
Ethanol
What technique helps improve contrast in light microscopy when visualizing pigmented microorganisms?
Gram staining
What color do Gram-positive cells appear as after the counterstain with red safranin?
Pink to red
What is the purpose of decolorizing with alcohol in the Gram staining technique?
To differentiate between Gram-positive and Gram-negative cells
How do Gram-negative bacteria appear after the Gram staining process?
Pink
What is the purpose of the iodine solution in the Gram staining technique?
To turn all cells purple
Which staining technique results in Gram-positive cells being purple and Gram-negative cells being colorless?
Differential staining
Why do staining techniques kill bacteria according to the provided text?
The text does not specify a reason
What is the key factor that limits our ability to see microorganisms clearly?
Resolution
Which type of microscopy visualizes specimens based on differences in contrast between the specimen and its surroundings?
Bright-field
What is the purpose of differential staining techniques in microbiology?
To differentiate between different types of microorganisms based on their staining properties
Which type of microscopy uses specialized optics to enhance the contrast of transparent specimens?
Phase-contrast
What is the primary function of Gram staining in microbiology?
To identify differences in cell wall structure
Which staining technique is commonly used to distinguish between Gram-positive and Gram-negative bacteria?
Gram staining
Test your knowledge on the heat fixing and staining process of cells. Learn about the steps involved like heat fixing, staining, and microscopy techniques used. Understand the differential (Gram) staining procedure and its results.
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