30 Questions
What is the principle behind Over-Representation Analysis?
Statistically evaluating the fraction of genes in a particular pathway that shows changes in expression
What does Gene Set Enrichment Analysis (GSEA) fall under?
Functional Class Scoring
What is the purpose of evaluating the fraction of genes in a pathway that shows changes in expression?
To determine if there is enrichment or overrepresentation in specific biological pathways
What percentage of genes showing big enrichment would indicate overrepresentation of a pathway?
20%
In Over-Representation Analysis, what does a significant p-value indicate?
The genes in a particular pathway are significantly affected
What does a poor enrichment and no overrepresentation in a pathway correspond to?
1% of genes belonging to the pathway
What is the focus of evaluating the fraction of genes in a particular pathway?
Determining if there is an enrichment or overrepresentation in specific biological pathways
In Over-Representation Analysis, what does the term 'functional subfunction' refer to?
Genes belonging to the same biological pathway
What is the purpose of pathway analysis?
To put genes in a functional context and identify dysregulated pathways
What is the focus of Over-representation Analysis (ORA)?
Identifying whether a pathway is overrepresented in the gene list
What is the main purpose of Gene Set Enrichment Analysis (GSEA)?
To determine whether a predefined set of genes shows statistically significant, concordant differences between two biological states
What does it mean to achieve data reduction in pathway analysis?
Reducing tens of thousands of genes to tens of pathways
What does evaluating the fraction of genes in a pathway that shows changes in expression provide?
More explanatory power about the modified functions/pathways/biological processes
What is the principle behind Over-representation Analysis (ORA)?
To compare the number of genes in a pathway with the number of genes in a random gene set
What is the primary goal of using GEO2R and WebGestalt in Gene Set Enrichment Analysis (GSEA)?
To perform GSEA and analyze the enrichment of gene sets
What is the primary goal of performing pathway analysis in the context of a microarray/RNA sequencing experiment?
To put the genes in a functional context and understand perturbed biological processes and dysregulated pathways
In pathway analysis, what is the purpose of Overrepresentation Analysis (ORA)?
To determine if a particular biological process or pathway is overrepresented in the list of genes of interest
What is the main focus of Gene Set Enrichment Analysis (GSEA) in pathway analysis?
To determine whether a predefined set of genes shows statistically significant, concordant differences between two biological states
What is the purpose of using GEO2R and WebGestalt in Gene Set Enrichment Analysis (GSEA)?
To perform differential gene expression analysis and identify enriched pathways
What does it mean to achieve data reduction in pathway analysis?
To reduce the thousands of genes to a smaller number of pathways for better interpretability
What does evaluating the fraction of genes in a pathway that shows changes in expression provide in pathway analysis?
Insights into the perturbed biological processes and dysregulated pathways
What is the focus of evaluating the fraction of genes in a particular pathway in pathway analysis?
To understand the impact of gene expression changes on the pathway's biological function
What does Over-Representation Analysis (ORA) aim to statistically evaluate?
The fraction of genes in a particular pathway that shows changes in expression
In Over-Representation Analysis, what indicates pathway enrichment?
A significantly affected group of genes belonging to a function or subfunction
What does a significant p-value in Over-Representation Analysis suggest?
The observed results are unlikely to be due to random chance
What is the focus of evaluating the fraction of genes in a pathway that shows changes in expression?
Determining if there is an enrichment or not in specific biological pathways
What does 'functional subfunction' refer to in Over-Representation Analysis?
Genes belonging to the same biological pathway
What percentage of genes showing big enrichment would indicate overrepresentation of a pathway?
20%
What is the primary goal of using Gene Set Enrichment Analysis (GSEA)?
To identify whether a set of genes shows statistically significant, concordant differences between two biological states
What is the purpose of evaluating the fraction of genes in a pathway that shows changes in expression?
To determine if there is an enrichment or not in specific biological pathways
Test your knowledge of research approaches in health biosciences with this quiz. Explore topics such as integrative mitochondrial biology, cellular and molecular medicine, and interdisciplinary health sciences. Challenge yourself to understand key concepts in health research under the guidance of Dr. Yan Burelle, a renowned professor at the University of Ottawa.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
