Haematology Methodologies Quiz

Study Notes

Methodologies in Haematology: Stains, Microscopy, and Quality Management

Romanowski stain is commonly used in haematology for most work, providing a red and blue stain to identify targets for further stains.
Kleihaur-Betke stain is used to calculate the relative size of a transplacental haemorrhage, assessing the survival rate of the haemorrhage.
Perls Prussian Blue stain is an iron stain used to assess iron stores in bone marrow and ferritin levels in patients.
Field stain is an acidified version of the Romanowski stain and is used to denature the cytoplasm of the cell slightly, often used to identify malaria.
Giemsa stain is used to identify parasitic worms and high eosinophil concentration, and can determine the type of worm through counting dots.
Conventional Epi-Fluorescence Microscopy is used for confirmatory tests, such as diagnosing chronic myeloid leukaemia.
Electron microscopy is used for treatment-related identification and to show fine morphological operations in leukaemia cells more clearly.
Confocal microscopy is used to visualize slides in 3D, showing how platelets work based on anatomy.
Haemocytometer is used to measure WBC and RBC counts, but can be fiddly and inaccurate.
Coulter Principle is a highly accurate method for counting and sizing RBCs based on the electrical charge across a gate.
Flow Cytometry is used to measure disease and monitor patients, such as HIV patients, to ensure medications are working and they are not becoming immunocompromised.
Quality management and control are critical in haematology to maintain consistent standards and prevent errors that could lead to misdiagnosis and mistreatment. ISO 15189 is a standard used for quality management in haematology labs.

Description

Test your knowledge of haematology methodologies with this quiz! From stains to microscopy to quality management, this quiz covers a range of topics that are crucial to understanding haematological processes. Learn about the different types of stains used to identify targets for further stains, the various microscopy techniques used to visualize cells and diagnose diseases, and the importance of quality management and control in haematology labs. Keywords: haematology, stains, microscopy, quality management, ISO 15189.