Podcast
Questions and Answers
What is the consensus sequence of the 9 bp DnaA boxes in oriC?
What is the consensus sequence of the 9 bp DnaA boxes in oriC?
5’-TTATCCACA-3'
What is the sequence of the 13-bp AT-rich region where initiation of replication commences?
What is the sequence of the 13-bp AT-rich region where initiation of replication commences?
5’-GATCTNTTTNTTT-3'
Which protein binds to the unwound region in DNA replication and is facilitated by DnaC?
Which protein binds to the unwound region in DNA replication and is facilitated by DnaC?
DnaB
What is the process called that denatures the DNA in the 13-mer sequences during replication?
What is the process called that denatures the DNA in the 13-mer sequences during replication?
Which protein prevents the renaturation of single-stranded DNA?
Which protein prevents the renaturation of single-stranded DNA?
What relieves the torsional stress caused by the unwinding of DNA during replication?
What relieves the torsional stress caused by the unwinding of DNA during replication?
What are the three models of DNA replication?
What are the three models of DNA replication?
Who showed that DNA replication is semiconservative?
Who showed that DNA replication is semiconservative?
What are the four steps involved in the general mechanism of DNA replication?
What are the four steps involved in the general mechanism of DNA replication?
How does DNA replication occur in terms of continuity and discontinuity?
How does DNA replication occur in terms of continuity and discontinuity?
What happens during Elongation in DNA replication?
What happens during Elongation in DNA replication?
What is the junction of the unwound molecules called in a replication bubble?
What is the junction of the unwound molecules called in a replication bubble?
How does leading-strand synthesis proceed?
How does leading-strand synthesis proceed?
What is the nature of lagging-strand synthesis?
What is the nature of lagging-strand synthesis?
How are Okazaki fragments eventually linked together?
How are Okazaki fragments eventually linked together?
Explain the Watson-Crick base-pairing during DNA replication.
Explain the Watson-Crick base-pairing during DNA replication.
In which direction does the phosphodiester bond formation occur during DNA replication?
In which direction does the phosphodiester bond formation occur during DNA replication?
List the three processes involved in DNA replication on prokaryotic cells.
List the three processes involved in DNA replication on prokaryotic cells.
What is the function of DNA gyrase in DNA replication in prokaryotic cells?
What is the function of DNA gyrase in DNA replication in prokaryotic cells?
Which DNA polymerase in prokaryotic cells is involved in new strand elongation?
Which DNA polymerase in prokaryotic cells is involved in new strand elongation?
What is the role of the 𝜀 (epsilon) subunit in DNA polymerase III?
What is the role of the 𝜀 (epsilon) subunit in DNA polymerase III?
Name the protein responsible for binding to single-stranded DNA in prokaryotic DNA replication.
Name the protein responsible for binding to single-stranded DNA in prokaryotic DNA replication.
What is the function of DnaB (helicase) in prokaryotic DNA replication?
What is the function of DnaB (helicase) in prokaryotic DNA replication?
Which DNA polymerase in prokaryotic cells is involved in primer excision and filling the gaps?
Which DNA polymerase in prokaryotic cells is involved in primer excision and filling the gaps?
What cleaves the flap structure at the Okazaki fragments during DNA replication?
What cleaves the flap structure at the Okazaki fragments during DNA replication?
What seals the gap in DNA after removal of the flap structure during DNA replication?
What seals the gap in DNA after removal of the flap structure during DNA replication?
Why is the RNA primer sequence at the 3′-hydroxyl end not replaceable by DNA polymerase during DNA replication?
Why is the RNA primer sequence at the 3′-hydroxyl end not replaceable by DNA polymerase during DNA replication?
What is the repetitive non-coding sequence of DNA at the 3′ end, crucial for preventing loss of genetic information during replication?
What is the repetitive non-coding sequence of DNA at the 3′ end, crucial for preventing loss of genetic information during replication?
What is the structure called where telomeric DNA is packed into to hide the chromosome ends?
What is the structure called where telomeric DNA is packed into to hide the chromosome ends?
What kind of proteins coat the telomeric DNA to protect it?
What kind of proteins coat the telomeric DNA to protect it?
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