17 Questions
What is the purpose of the iodine solution in Gram staining?
To attach crystal violet to the cell
What role does the decolorizing solution play in Gram staining?
To remove crystal violet from the cell
Which of the following is true regarding capsule staining?
Capsules may be removed if staining is not done gently
What distinguishes endospores from other cell structures?
Endospores are resistant to harsh conditions
Which staining technique is used to observe structures like capsules and endospores?
Special staining
Why is an alcohol solution applied after the iodine in Gram staining?
To remove the purple color from the cell
What is the main purpose of a simple stain?
To color the entire cells evenly and observe the shape of the cell
Which chemical is not commonly used in chemical fixation to stabilize and lock macromolecules in position?
Methylene blue
What staining technique allows identification of major bacterial groups by using more than one color dye?
Differential staining
What is the main disadvantage of heat fixation when preparing bacterial samples for staining?
It can destroy the internal structure and enzymes due to heat
Which type of stain was invented by Hans Christian Gram in 1884?
Gram stain
What is the main function of crystal violet in a Gram stain?
To bind to cells as the primary stain
What is the primary purpose of endospore staining in microbiology?
To observe the capsules surrounding bacterial cells
What is the significance of counterstaining with safranin in the described staining method?
To stain endospores pink while leaving cells green
Why is fixation crucial in preparing specimens for microscopy?
To kill and preserve the structure of cells and tissues
Which staining technique would be most suitable for observing the external structures of bacterial cells?
Flagella staining
What is the primary benefit of visualizing endospores using special staining techniques?
To detect highly resistant structures within bacterial cells
Learn about the steps involved in the Gram staining process, including the application of iodine solution, crystal violet, decolorizing solution, and safranin. Understand how each solution interacts with bacterial cells during the staining process.
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