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Questions and Answers
What is the purpose of the iodine solution in Gram staining?
What is the purpose of the iodine solution in Gram staining?
- To remove purple color from the cell
- To apply a pink counterstain
- To rinse away excess crystal violet
- To attach crystal violet to the cell (correct)
What role does the decolorizing solution play in Gram staining?
What role does the decolorizing solution play in Gram staining?
- To remove crystal violet from the cell (correct)
- To rinse away the iodine solution
- To attach safranin to the cell
- To produce large crystal violet iodine complexes
Which of the following is true regarding capsule staining?
Which of the following is true regarding capsule staining?
- Capsules may be removed if staining is not done gently (correct)
- Dye sticks to capsules and cells with equal affinity
- Capsules are water-resistant structures
- Capsules are easily observable under a microscope
What distinguishes endospores from other cell structures?
What distinguishes endospores from other cell structures?
Which staining technique is used to observe structures like capsules and endospores?
Which staining technique is used to observe structures like capsules and endospores?
Why is an alcohol solution applied after the iodine in Gram staining?
Why is an alcohol solution applied after the iodine in Gram staining?
What is the main purpose of a simple stain?
What is the main purpose of a simple stain?
Which chemical is not commonly used in chemical fixation to stabilize and lock macromolecules in position?
Which chemical is not commonly used in chemical fixation to stabilize and lock macromolecules in position?
What staining technique allows identification of major bacterial groups by using more than one color dye?
What staining technique allows identification of major bacterial groups by using more than one color dye?
What is the main disadvantage of heat fixation when preparing bacterial samples for staining?
What is the main disadvantage of heat fixation when preparing bacterial samples for staining?
Which type of stain was invented by Hans Christian Gram in 1884?
Which type of stain was invented by Hans Christian Gram in 1884?
What is the main function of crystal violet in a Gram stain?
What is the main function of crystal violet in a Gram stain?
What is the primary purpose of endospore staining in microbiology?
What is the primary purpose of endospore staining in microbiology?
What is the significance of counterstaining with safranin in the described staining method?
What is the significance of counterstaining with safranin in the described staining method?
Why is fixation crucial in preparing specimens for microscopy?
Why is fixation crucial in preparing specimens for microscopy?
Which staining technique would be most suitable for observing the external structures of bacterial cells?
Which staining technique would be most suitable for observing the external structures of bacterial cells?
What is the primary benefit of visualizing endospores using special staining techniques?
What is the primary benefit of visualizing endospores using special staining techniques?
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