Gram Stain Procedure

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8 Questions

What is the primary purpose of the Gram stain?

To distinguish bacteria as Gram-positive or Gram-negative

What is the color of Gram-positive cells after the Gram stain procedure?

Purple

What is the purpose of the decolorizer in the Gram stain procedure?

To remove excess stain from the bacterial cells

What is the total magnification used to view the Gram-stained slide?

1000X

What is the recommended time to wait after applying the crystal violet to the slide?

15 seconds

What is the purpose of the counterstain in the Gram stain procedure?

To add color to Gram-negative cells

Why is it important to blot the slide with absorbent paper after staining?

To prevent the cells from coming off the slide

What type of microscope is used to view the Gram-stained slide?

Brightfield microscope

Test your knowledge of the Gram stain procedure, a differential staining technique used to categorize bacteria as Gram-positive or Gram-negative. Learn about the steps involved and the supplies needed.

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