Gram Stain Procedure
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Questions and Answers

What is the primary purpose of the Gram stain?

  • To determine the shape of bacterial cells
  • To distinguish bacteria as Gram-positive or Gram-negative (correct)
  • To identify the type of media used for bacterial growth
  • To classify bacteria as aerobic or anaerobic

    • What is the color of Gram-positive cells after the Gram stain procedure?

  • Green
  • Purple (correct)
  • Red to pink
  • Blue

    • What is the purpose of the decolorizer in the Gram stain procedure?

  • To add color to the bacterial cells
  • To fix the bacterial cells to the slide
  • To enhance the contrast between Gram-positive and Gram-negative cells
  • To remove excess stain from the bacterial cells (correct)

    • What is the total magnification used to view the Gram-stained slide?

    <p>1000X</p> Signup and view all the answers

    What is the recommended time to wait after applying the crystal violet to the slide?

    <p>15 seconds</p> Signup and view all the answers

    What is the purpose of the counterstain in the Gram stain procedure?

    <p>To add color to Gram-negative cells</p> Signup and view all the answers

    Why is it important to blot the slide with absorbent paper after staining?

    <p>To prevent the cells from coming off the slide</p> Signup and view all the answers

    What type of microscope is used to view the Gram-stained slide?

    <p>Brightfield microscope</p> Signup and view all the answers

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