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Questions and Answers
Which of the following is a characteristic of gram-positive bacteria?
Which of the following is a characteristic of gram-positive bacteria?
Which of the following genera is classified as a gram-positive bacterium?
Which of the following genera is classified as a gram-positive bacterium?
What distinguishes the domains of Bacteria from Archaea?
What distinguishes the domains of Bacteria from Archaea?
Which of the following statements about gram-negative bacteria is correct?
Which of the following statements about gram-negative bacteria is correct?
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Which group of bacteria includes both pathogenic and non-pathogenic species?
Which group of bacteria includes both pathogenic and non-pathogenic species?
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What is a significant characteristic of Gram-negative bacteria?
What is a significant characteristic of Gram-negative bacteria?
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Which genus is known for its probiotic properties and can outcompete pathogens?
Which genus is known for its probiotic properties and can outcompete pathogens?
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What type of infections is Staphylococcus aureus commonly associated with?
What type of infections is Staphylococcus aureus commonly associated with?
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Which of the following is a characteristic of the Bacillota phylum?
Which of the following is a characteristic of the Bacillota phylum?
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Which lactic acid bacteria is commonly used in the production of Emmenthal cheese?
Which lactic acid bacteria is commonly used in the production of Emmenthal cheese?
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Which disease is NOT caused by Streptococcus pyogenes?
Which disease is NOT caused by Streptococcus pyogenes?
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Which of the following is a common habitat for Lactobacillus?
Which of the following is a common habitat for Lactobacillus?
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What is a common consequence of lactic acid production by Lactobacillus?
What is a common consequence of lactic acid production by Lactobacillus?
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What role does protein A play in Staphylococcus aureus's virulence?
What role does protein A play in Staphylococcus aureus's virulence?
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What is a characteristic feature of Bacillus thuringiensis?
What is a characteristic feature of Bacillus thuringiensis?
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Which species of Clostridium is known for its association with botulism?
Which species of Clostridium is known for its association with botulism?
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Which transmission route is associated with Bacillus anthracis?
Which transmission route is associated with Bacillus anthracis?
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How is antibiotic resistance typically represented in Staphylococcus aureus?
How is antibiotic resistance typically represented in Staphylococcus aureus?
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What is the primary effect of the botulinum toxin produced by Clostridium botulinum?
What is the primary effect of the botulinum toxin produced by Clostridium botulinum?
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What type of bacteria does the Phylum Actinomycetota primarily consist of?
What type of bacteria does the Phylum Actinomycetota primarily consist of?
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Which of the following diseases is not directly caused by mycobacterial pathogens?
Which of the following diseases is not directly caused by mycobacterial pathogens?
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Which statement is correct regarding Streptomyces?
Which statement is correct regarding Streptomyces?
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What unique characteristic does Mycobacterium have compared to other bacteria?
What unique characteristic does Mycobacterium have compared to other bacteria?
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Study Notes
Gram Positives
- Gram-positive bacteria have a thick layer of peptidoglycan in their cell walls
- They lack an outer membrane
- They often contain teichoic acids
- Examples include Bacillus, Streptococcus, and Staphylococcus
Classifying Prokaryotes
- Prokaryotes are categorized into two domains: Bacteria and Archaea
- Bacteria are further divided into phyla, such as Pseudomonadota, Bacteroidota, Chlamydiota, Cyanobacteriota, Spirochetota, Bacillota, and Actinomycetota
- Bacillota (Firmicutes) is a significant component of the human gut microbiome
- Actinomycetota (Actinobacteria) is associated with soil and nitrogen fixation
- Pseudomonadota and non-Pseudomonadota are related groups of Gram-negative bacteria.
Gram Positive vs. Gram Negative
- Gram-positive bacteria have a thick peptidoglycan layer and lack an outer membrane.
- Gram-negative bacteria have a thin peptidoglycan layer and an outer membrane containing lipopolysaccharides
- Gram-positive bacteria often have teichoic acid in their cell wall.
Phylum: Bacillota (Firmicutes)
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Low GC content
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Often obligate or facultative anaerobes
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Important component of the human intestinal microbiome
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Number may decrease with age and is possibly linked to obesity
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Order: Lactobacillales
- Low GC content
- Bacteria that tolerate or exist in acidic conditions
- Examples: Streptococcus, Lactobacillus,
- Used in food industry (yogurt, cheese, wine) & as probiotics
- Some species can cause disease
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Order: Bacillales
- Common in soil
- Anaerobic, facultative aerobic
- Spore-formers
Staphylococcus aureus
- Causes a wide range of diseases, including skin infections, food poisoning, and toxic shock syndrome
- Widely distributed in the environment and 25% of the population are carriers
- Causes hospital acquired infections
- Produces toxins, such as enterotoxins, leading to food poisoning and other diseases
- Resistant to some antibiotics (MRSA)
Bacillus thuringiensis
- Produces insecticidal toxins used as natural pesticides
- Pesticides based on these toxins are considered safe for the environment
- Used to control insect pests, particularly blackflies which act as vectors
- Used in genetically engineered crops (GM crops)
Bacillus anthracis
- Causes anthrax, a serious disease that can affect livestock and humans.
- Different forms of anthrax (cutaneous, pulmonary, gastrointestinal) have different levels of mortality
- Can be weaponized
Clostridium botulinum
- This bacterium creates botulism, especially in canned foods.
- Its toxin affects the neuromuscular system, causing muscle weakness and paralysis.
- Antitoxin is used to counteract it, and this bacterium also has medical applications, including treatment of muscle spasms and cosmetic uses.
Phylum: Actinomycetota (Actinobacteria)
- High GC content
- Mostly terrestrial or lives in aquatic environments.
- Important in the soil, nitrogen fixation
- Several are pathogens
Streptomyces
- Largest genus of actinobacteria
- A major source of antibiotics, including streptomycin, chloramphenicol, and tetracycline
- Soil dwelling, aerobic and spore-forming
- Produce geosmin, a compound that gives soil its earthy smell
Mycobacterium
- Cannot be stained using Gram staining technique
- Slow-growing aerobic bacteria
- Two notable pathogens: Mycobacterium tuberculosis (TB), a lung infection, and Mycobacterium leprae (leprosy), affecting the skin and peripheral nerves.
- TB & Leprosy are socially stigmatic infections due to the slow-growing nature of the bacteria
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Description
Test your knowledge on the characteristics of Gram-positive and Gram-negative bacteria. This quiz covers the structural differences, classification of prokaryotes, and examples of significant bacterial groups. Understand the role these bacteria play in various ecosystems and human health.