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Questions and Answers
Which of the following is NOT a risk factor for invasive Neisseria meningitidis disease?
Which of the following is NOT a risk factor for invasive Neisseria meningitidis disease?
What is the primary mode of transmission for Neisseria meningitidis?
What is the primary mode of transmission for Neisseria meningitidis?
Which of the following is a characteristic of Neisseria meningitidis bacteria?
Which of the following is a characteristic of Neisseria meningitidis bacteria?
What is the typical incubation period for Neisseria meningitidis infection?
What is the typical incubation period for Neisseria meningitidis infection?
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What is a possible consequence of Neisseria meningitidis infection?
What is a possible consequence of Neisseria meningitidis infection?
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Which age group is most susceptible to invasive Neisseria meningitidis disease?
Which age group is most susceptible to invasive Neisseria meningitidis disease?
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What is the name given to the invasive form of Neisseria meningitidis infection that affects the bloodstream?
What is the name given to the invasive form of Neisseria meningitidis infection that affects the bloodstream?
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How can Neisseria meningitidis spread in crowded environments?
How can Neisseria meningitidis spread in crowded environments?
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What is a distinctive characteristic of Neisseria species that helps differentiate them from other Gram-negative bacteria?
What is a distinctive characteristic of Neisseria species that helps differentiate them from other Gram-negative bacteria?
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Which of the following is NOT a general characteristic of the clinically significant Gram-negative cocci Neisseria and Moraxella?
Which of the following is NOT a general characteristic of the clinically significant Gram-negative cocci Neisseria and Moraxella?
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What is true about the growth conditions of Neisseria species?
What is true about the growth conditions of Neisseria species?
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How are Neisseria species often identified?
How are Neisseria species often identified?
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What is the significance of pili in Neisseria species?
What is the significance of pili in Neisseria species?
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Which of the following is a true statement regarding the growth of Neisseria species?
Which of the following is a true statement regarding the growth of Neisseria species?
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What is the relationship between the morphology of Neisseria species and their capsule production?
What is the relationship between the morphology of Neisseria species and their capsule production?
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Some Neisseria species are described as capnophilic. What does this mean?
Some Neisseria species are described as capnophilic. What does this mean?
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Which antibiotic is a recommended treatment option for patients allergic to penicillin?
Which antibiotic is a recommended treatment option for patients allergic to penicillin?
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What are the two types of quadrivalent polysaccharide-protein conjugated vaccines available for Neisseria meningitidis?
What are the two types of quadrivalent polysaccharide-protein conjugated vaccines available for Neisseria meningitidis?
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Who are the recommended recipients for the Neisseria meningitidis vaccine?
Who are the recommended recipients for the Neisseria meningitidis vaccine?
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What is the reason for using Azithromycin as a treatment option for Neisseria meningitidis?
What is the reason for using Azithromycin as a treatment option for Neisseria meningitidis?
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Which of the following serogroups are NOT included in the quadrivalent polysaccharide-protein conjugated vaccines?
Which of the following serogroups are NOT included in the quadrivalent polysaccharide-protein conjugated vaccines?
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What are conjugated vaccines of proteins from other bacteria bound to Neisseria meningitides group B outer membrane proteins referred to as?
What are conjugated vaccines of proteins from other bacteria bound to Neisseria meningitides group B outer membrane proteins referred to as?
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What is the recommended treatment option for confirmed meningitis?
What is the recommended treatment option for confirmed meningitis?
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What is the primary reason for recommending vaccination against Neisseria meningitidis for laboratory scientists?
What is the primary reason for recommending vaccination against Neisseria meningitidis for laboratory scientists?
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How do Neisseria species aid in exchange of genetic material from cell to cell?
How do Neisseria species aid in exchange of genetic material from cell to cell?
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Which of the following virulence factors of Neisseria species helps them evade the host's immune system by preventing phagocytosis?
Which of the following virulence factors of Neisseria species helps them evade the host's immune system by preventing phagocytosis?
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What is the primary function of lipooligosaccharides (LOS) in Neisseria species?
What is the primary function of lipooligosaccharides (LOS) in Neisseria species?
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Which virulence factor of Neisseria species plays a role in evading the host's immune response by preventing the binding of effective antibodies?
Which virulence factor of Neisseria species plays a role in evading the host's immune response by preventing the binding of effective antibodies?
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How does Protein II (Opa) contribute to the virulence of Neisseria species?
How does Protein II (Opa) contribute to the virulence of Neisseria species?
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Which of the following Neisseria species is always pathogenic when present?
Which of the following Neisseria species is always pathogenic when present?
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Which of the following is a known reservoir for Neisseria meningitidis?
Which of the following is a known reservoir for Neisseria meningitidis?
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What is the role of Protein I (Por) in Neisseria species?
What is the role of Protein I (Por) in Neisseria species?
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What is the primary function of receptors for human transferrin in Neisseria species?
What is the primary function of receptors for human transferrin in Neisseria species?
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Which of the following is NOT a known virulence factor of Neisseria species?
Which of the following is NOT a known virulence factor of Neisseria species?
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What is the primary human pathogen caused by Neisseria species?
What is the primary human pathogen caused by Neisseria species?
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Which of the following Neisseria species is also known as gonococci?
Which of the following Neisseria species is also known as gonococci?
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What is the primary role of IgA protease in Neisseria species?
What is the primary role of IgA protease in Neisseria species?
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Neisseria species can be found as a commensal inhabitant of which of the following biological locations in humans?
Neisseria species can be found as a commensal inhabitant of which of the following biological locations in humans?
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What is the primary factor that determines whether a person infected with Neisseria meningitidis will develop meningococcal disease?
What is the primary factor that determines whether a person infected with Neisseria meningitidis will develop meningococcal disease?
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Which population is at greater risk for developing serious meningococcal disease?
Which population is at greater risk for developing serious meningococcal disease?
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Which of the following symptoms is NOT commonly associated with meningitis?
Which of the following symptoms is NOT commonly associated with meningitis?
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Which condition can increase the risk of developing meningococcal disease?
Which condition can increase the risk of developing meningococcal disease?
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What is a characteristic symptom of purulent meningitis?
What is a characteristic symptom of purulent meningitis?
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Which of the following statements about meningococcus disease is true?
Which of the following statements about meningococcus disease is true?
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What is the primary route of transmission for Neisseria gonorrhoeae infections?
What is the primary route of transmission for Neisseria gonorrhoeae infections?
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Which of the following is a common site of infection in women infected with Neisseria gonorrhoeae?
Which of the following is a common site of infection in women infected with Neisseria gonorrhoeae?
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What percentage of meningococcal B strains does chemoprophylaxis provide short-term protection against?
What percentage of meningococcal B strains does chemoprophylaxis provide short-term protection against?
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Which group is not recommended for chemoprophylaxis during outbreaks?
Which group is not recommended for chemoprophylaxis during outbreaks?
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Which symptom is commonly associated with infections in women caused by Neisseria gonorrhoeae?
Which symptom is commonly associated with infections in women caused by Neisseria gonorrhoeae?
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What is the primary reservoir for Neisseria gonorrhoeae?
What is the primary reservoir for Neisseria gonorrhoeae?
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What form of delivery is used for administering chemoprophylaxis against meningococcal B strains?
What form of delivery is used for administering chemoprophylaxis against meningococcal B strains?
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What is the common type of discharge in women with Neisseria gonorrhoeae infections?
What is the common type of discharge in women with Neisseria gonorrhoeae infections?
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Which of the following is a possible symptom of Neisseria gonorrhoeae infection in women?
Which of the following is a possible symptom of Neisseria gonorrhoeae infection in women?
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What percentage of United States meningococcal B strains does chemoprophylaxis NOT provide protection against?
What percentage of United States meningococcal B strains does chemoprophylaxis NOT provide protection against?
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Flashcards
Neisseria
Neisseria
A genus of Gram-negative cocci known for causing infections.
Moraxella
Moraxella
A genus of Gram-negative cocci associated with respiratory infections.
Gram-negative cocci
Gram-negative cocci
Bacteria that are spherical and do not retain the crystal violet stain used in Gram staining.
Catalase positive
Catalase positive
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Oxidase positive
Oxidase positive
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Capnophilic
Capnophilic
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Pili
Pili
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Colony types T1-T5
Colony types T1-T5
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Populations at risk for meningitis
Populations at risk for meningitis
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Symptoms of meningitis
Symptoms of meningitis
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Septicemia
Septicemia
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Meningococcus disease rarity
Meningococcus disease rarity
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Conditions increasing meningitis risk
Conditions increasing meningitis risk
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Fastidious organism
Fastidious organism
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Epidemic meningitis
Epidemic meningitis
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Meningococcemia
Meningococcemia
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At-risk populations
At-risk populations
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Asplenic patients
Asplenic patients
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Complement deficiencies
Complement deficiencies
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Incubation period
Incubation period
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Crowded living conditions
Crowded living conditions
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Neisseria meningitidis treatment
Neisseria meningitidis treatment
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Chloramphenicol
Chloramphenicol
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Third-generation cephalosporins
Third-generation cephalosporins
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Chemoprophylaxis
Chemoprophylaxis
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Quadrivalent meningococcal vaccines
Quadrivalent meningococcal vaccines
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Serogroup B vaccines
Serogroup B vaccines
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Vaccine recommendations
Vaccine recommendations
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Close contacts prophylaxis
Close contacts prophylaxis
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Virulence Factors
Virulence Factors
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Capsule
Capsule
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Phagocytosis
Phagocytosis
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Mucous Membranes
Mucous Membranes
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Neisseria meningitidis
Neisseria meningitidis
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Neisseria gonorrhea
Neisseria gonorrhea
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Antigenic Variation
Antigenic Variation
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Outer Membrane Proteins
Outer Membrane Proteins
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Sex Pili
Sex Pili
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IgA Protease
IgA Protease
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Commensal Inhabitant
Commensal Inhabitant
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Transmission
Transmission
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Tissue Damage
Tissue Damage
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Iron Competition
Iron Competition
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Meningococcal B strains
Meningococcal B strains
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Dose series
Dose series
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Primary reservoir for gonorrhea
Primary reservoir for gonorrhea
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Transmission of gonorrhea
Transmission of gonorrhea
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Infection site in women
Infection site in women
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Symptoms of gonorrhea in women
Symptoms of gonorrhea in women
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Cervical/vaginal discharge
Cervical/vaginal discharge
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Dysuria
Dysuria
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Study Notes
Gram-Negative Cocci
- Gram-negative cocci are usually diplococci
- Catalase positive
- Oxidase positive
- Most grow on sheep and chocolate agar
- Identified by CTA sugar fermentation patterns
- Aerobic
- Nonmotile
- Non-spore-forming
Pathogenicity and Host Range
- Table 17-1 shows pathogenicity and host range for Neisseria and Moraxella species.
- Table 17-5 details characteristics of significant species
- N. gonorrhoeae: primary pathogen, humans only
- N. meningitidis: primary pathogen, humans only
- N. lactamica, N. cinerea, N. sicca, N. flavescens: opportunistic pathogens, warm-blooded animals; N. subflava, N. mucosa: opportunistic pathogens, warm-blooded animals
- Moraxella catarrhalis: opportunistic pathogen, humans only
Cellular Structure of Pathogenic Neisseria
- The cell wall of pathogenic Neisseria has components like LOS and pili
Virulence Factors of Neisseria
- Pili aid in attachment and prevent phagocytosis; exchange of genetic material
- Capsule prevents phagocytosis
- Cell-outer membrane proteins cause antigenic variation and prevent effective antibody binding
- Major outer membrane porin proteins (Por, Opa, Rmp) block host serum bactericidal action and facilitate adherence
- Lipooligosaccharides (LOS) lead to tissue damage
- IgA protease cleaves IgA at mucosal surfaces
- Receptors for human transferrin allow for iron competition
Neisseria meningitidis
- Found only in humans
- Normal flora of the upper respiratory tract and urogenital tracts
- May be a commensal or an invasive pathogen
- Infections include endemic and epidemic meningitis and meningococcemia
Neisseria meningitidis: Epidemiology
- Spread through crowded living conditions (military recruits, college students)
- Transmission via respiratory droplets from carriers to new hosts
- Highest incidence in infants and adolescents
- Populations at risk of invasive disease include asplenic patients and those with complement deficiencies
- Factors contributing to spread include crowded living conditions and military/college settings
Neisseria meningitidis: Clinical Infections
- Symptoms include abrupt onset of headaches, stiff neck, fever, etc.
- Death may occur within 12-48 hours after symptoms appear
- Meningococcemia is characterized by purpuric skin lesions and may or may not accompany meningitis.
- Complications may include arthritis, pericarditis, pneumonia, conjuntivitis, and urethritis
- Can lead to Waterhouse-Friderichsen syndrome (adrenal hemorrhage)
Laboratory Diagnosis of Neisseria meningitidis
- Specimens like CSF, blood, or nasopharyngeal swabs are used
- Need to grow on SBA and chocolate agar requiring CO2
- Identification is based on colony morphology, microscopic morphology, and oxidase test
Neisseria gonorrhoeae
- Humans are the only natural hosts for gonorrhea
- Urogenital tract, pharynx, and conjunctiva of the eye are common infection sites
- Infections in men: Acute urethritis, Prostatitis, epididymitis
- Infections in women: Endocervix (common), cervical or vaginal discharge, dysuria, lower abdominal pain, vaginal bleeding; untreated infections can lead to PID and sterility; complications may also include ectopic pregnancy and/or perihepatitis (Fitz-Hugh-Curtis syndrome)
- Disseminated infections can lead to purulent arthritis and septicemia
- Blood-borne disseminated infections can cause fever and rash on extremities.
Neisseria gonorrhoeae: Identification
- Direct microscopic examinations use Gram stains to look for gram-negative intracellular/extracellular diplococci
- Cultures are crucial for confirmation using enriched media (like Modified Thayer-Martin (MTM) agar), with 35-37°C incubation and 3-10% CO2
Neisseria gonorrhoeae: Treatment and Resistance
- Penicillin for confirmed meningitis use
- Chloramphenicol, third-generation cephalosporins, azithromycin, ciprofloxacin, or sulfonamides for penicillin-allergic patients.
- Chemoprophylaxis with rifampin, ciprofloxacin, or sulfonamides may be used for people in close contact.
- Antimicrobial-resistant strains exist (PPNG, CMRNG, TRNG)
- Fluoroquinolones are no longer recommended due to resistance.
- Ceftriaxone is currently the recommended treatment.
Nonpathogenic Neisseria Species
- Gram-negative cocci
- Normal parts of upper respiratory microbiota.
- Include N. bacilliformis, N. canis, N. cinerea, N. flavescens, N. elongata, N. mucosa, N. ovis, N. shayeganii, N. sicca, N. subflava, N. wadsworthii, N. zoodegmatis
- Clinical diagnosis often involves growth in culture
Moraxella Species
- Family Moraxellaceae
- 22 species (constantly revised), identified by being catalase-positive and oxidase-positive
- Found in the upper respiratory tract as normal microbiota
- One commercially important species is Moraxella catarrhalis, found in children and older adults and generally identified by being catalase positive and oxidase positive
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Description
This quiz covers the characteristics, pathogenicity, and virulence factors of Gram-negative cocci, specifically focusing on Neisseria species. It discusses their cellular structure, growth conditions, and host range. Test your knowledge on these important microorganisms and their roles in human health and disease.