GPCR Activation and Cellular Signaling

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Questions and Answers

What is the primary function of GPCR activation in cellular signaling?

  • Production of second messengers (correct)
  • Conversion of ATP to cAMP
  • Degradation of second messengers
  • Opening of gated ion channels

Which of the following receptors leads to the activation of phosphoinositide second messenger system?

  • Insulin receptor
  • GABA receptor
  • a-adrenergic receptor (correct)
  • b-adrenergic receptor

Which G-protein is associated with adenylate cyclase activation?

  • Gqa
  • Gtg
  • Gti
  • Gsa (correct)

What is the resultant second messenger when phospholipase C is activated?

<p>IP3 and DAG (A)</p> Signup and view all the answers

Which protein kinase is activated by adenylate cyclase?

<p>PKA (D)</p> Signup and view all the answers

In the context of Gibbs free energy, what is ΔG°?

<p>Free energy change under standard conditions (D)</p> Signup and view all the answers

Which process contributes to the termination of signaling effects?

<p>GTPase activation (C)</p> Signup and view all the answers

According to the laws of thermodynamics, what is stated in the second law?

<p>Every energy transfer increases entropy (C)</p> Signup and view all the answers

Which intermediate is formed after isocitrate in the TCA cycle?

<p>A-ketoglutarate (D)</p> Signup and view all the answers

Which enzyme catalyzes the conversion of malate to oxaloacetate in the TCA cycle?

<p>Malate DH (D)</p> Signup and view all the answers

What stimulates gluconeogenesis during low glucose levels?

<p>Glucagon (D)</p> Signup and view all the answers

Which tissue is the primary site for gluconeogenesis?

<p>Liver (D)</p> Signup and view all the answers

What is produced during the conversion of succinate to fumarate in the TCA cycle?

<p>FADH2 (A)</p> Signup and view all the answers

Which of the following statements about the TCA cycle is NOT correct?

<p>NADH is not produced in the CO2 producing steps. (D)</p> Signup and view all the answers

Which precursor is involved in gluconeogenesis and primarily sourced from muscle tissue?

<p>Alanine (A)</p> Signup and view all the answers

Which of the following regulates the TCA cycle by inhibiting certain steps?

<p>ATP (A)</p> Signup and view all the answers

Which enzymes catalyze the irreversible steps of gluconeogenesis?

<p>PEP carboxykinase (A), F-1,6 bisphosphatase (B), Pyruvate carboxylase (C)</p> Signup and view all the answers

How many ATP equivalents are required to synthesize one molecule of glucose?

<p>4 ATP, 2 GTP, and 2 NADH (B)</p> Signup and view all the answers

Which of the following substances inhibits Fructose 1,6-bisphosphatase?

<p>AMP (D)</p> Signup and view all the answers

Which complex in the electron transport chain uses FMN as a cofactor?

<p>Complex I (B)</p> Signup and view all the answers

What is the role of CoQ in the electron transport chain?

<p>It carries electrons from Complexes I and II to Complex III (B)</p> Signup and view all the answers

What activates gluconeogenesis through allosteric regulation?

<p>Acetyl CoA (B)</p> Signup and view all the answers

Which electron carrier is hydrophilic and found in the intermembrane space?

<p>Cytochrome C (A)</p> Signup and view all the answers

What is primarily responsible for the generation of the electrochemical gradient during electron transport?

<p>Transfer of electrons through complexes (A)</p> Signup and view all the answers

What distinguishes an endergonic reaction from an exergonic reaction?

<p>Endergonic reactions have a positive ΔG, while exergonic reactions have a negative ΔG. (A)</p> Signup and view all the answers

How is the actual ΔG of a cellular reaction calculated using ΔG° and cellular concentrations?

<p>By using the equation ΔG = ΔG° + RT ln(Q) where Q is the reaction quotient. (B)</p> Signup and view all the answers

Which term describes compounds with free energy of hydrolysis equal to or higher than ATP?

<p>High energy compounds (D)</p> Signup and view all the answers

What is the role of the various phosphate groups in ATP?

<p>They contribute to the high energy bonds due to significant electrostatic repulsion. (A)</p> Signup and view all the answers

What characterizes substrate-level phosphorylation?

<p>It couples with the release of free energy to produce ATP or GTP. (A)</p> Signup and view all the answers

Which GLUT transporter is specifically insulin-dependent?

<p>GLUT-4 (D)</p> Signup and view all the answers

Which of the following is NOT a rate-limiting enzyme in glycolysis?

<p>Lactate dehydrogenase (B)</p> Signup and view all the answers

What is the total energy output of glycolysis under aerobic conditions?

<p>6-8 ATP molecules (B)</p> Signup and view all the answers

What is the action of Atractyloside on the adenine nucleotide transporter?

<p>It binds to the outward-facing portion of the transporter. (A)</p> Signup and view all the answers

Which of the following statements correctly differentiates inhibitors of the ETC from uncouplers of oxidative phosphorylation?

<p>Uncouplers increase oxygen consumption while inhibitors decrease it. (C), Both inhibitors and uncouplers lead to a decrease in ATP synthesis. (D)</p> Signup and view all the answers

How does thermogenin function in brown adipose tissue?

<p>It uncouples the H+ gradient from ATP synthesis to generate heat. (B)</p> Signup and view all the answers

What role do ionophores play in cellular respiration?

<p>They form channels or act as mobile carriers for ions. (B)</p> Signup and view all the answers

What is the P/O ratio, and what does it represent in oxidative phosphorylation?

<p>It measures the efficiency of ATP production relative to oxygen consumption. (C)</p> Signup and view all the answers

Which shuttle system synthesizes more ATP from cytoplasmic NADH?

<p>Glycerophosphate shuttle produces 2 ATP per NADH oxidized. (C), Malate-aspartate shuttle produces 3 ATP per NADH oxidized. (D)</p> Signup and view all the answers

What effect do inhibitors of the electron transport chain have on ATP synthesis?

<p>They decrease ATP synthesis by blocking electron transfer. (A)</p> Signup and view all the answers

What is characteristic of uncouplers compared to inhibitors of the ETC?

<p>Uncouplers disrupt membrane gradients, increasing electron transport. (C)</p> Signup and view all the answers

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