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Questions and Answers
What is the product of the reaction catalyzed by hexokinase?
What is the product of the reaction catalyzed by hexokinase?
Which enzyme catalyzes the conversion of Fructose-6-phosphate to Fructose-1,6-bisphosphate?
Which enzyme catalyzes the conversion of Fructose-6-phosphate to Fructose-1,6-bisphosphate?
What products are formed from the cleavage of Fructose-1,6-bisphosphate?
What products are formed from the cleavage of Fructose-1,6-bisphosphate?
Which reaction is catalyzed by Enolase?
Which reaction is catalyzed by Enolase?
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What type of reaction does Phosphoglycerate kinase perform?
What type of reaction does Phosphoglycerate kinase perform?
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Study Notes
Glycolysis: Key Steps and Enzymes
- Glucose is phosphorylated to Glucose-6-phosphate (G6P) by the enzyme Hexokinase. This is an irreversible step.
- Glucose-6-phosphate (G6P) is isomerized to Fructose-6-phosphate (F6P) by the enzyme Phosphoglucoisomerase.
- Fructose-6-phosphate (F6P) is phosphorylated to Fructose-1,6-bisphosphate (F1,6BP) using ATP by Phosphofructokinase-1 (PFK-1). This is another irreversible step.
- Fructose-1,6-bisphosphate (F1,6BP) is cleaved into Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde-3-phosphate (G3P) by the enzyme Aldolase.
- Dihydroxyacetone phosphate (DHAP) is isomerized to Glyceraldehyde-3-phosphate (G3P) by Triosephosphate isomerase (TIM).
- Glyceraldehyde-3-phosphate (G3P) is oxidized and phosphorylated to 1,3-Bisphosphoglycerate (1,3BPG) by Glyceraldehyde-3-phosphate dehydrogenase.
- 1,3-Bisphosphoglycerate (1,3BPG) undergoes substrate-level phosphorylation, producing 3-Phosphoglycerate (3PG) and ATP using Phosphoglycerate kinase.
- 3-Phosphoglycerate (3PG) is isomerized to 2-Phosphoglycerate (2PG) by Phosphoglyceratemutase.
- 2-Phosphoglycerate (2PG) loses a water molecule (dehydration) forming Phosphoenolpyruvate (PEP) with Enolase.
- Phosphoenolpyruvate (PEP) produces Pyruvate and ATP through substrate-level phosphorylation by Pyruvate kinase.
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Description
Explore the crucial steps of glycolysis and the enzymes involved in each stage. This quiz covers the conversion of glucose to pyruvate, emphasizing irreversible steps and key intermediates. Test your knowledge on this fundamental metabolic pathway.
