Gluconeogenesis Enzymes Quiz
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Questions and Answers

What is the location of pyruvate carboxylase responsible for pyruvate carboxylation?

  • In the mitochondrial matrix (correct)
  • In the nucleus
  • In the endoplasmic reticulum
  • In the cytoplasm
  • What is the source of energy required for pyruvate carboxylation?

  • Hydrolysis of ATP (correct)
  • Hydrolysis of GTP
  • Hydrolysis of ADP
  • Hydrolysis of AMP
  • What is the product of C2 phosphorylation by GTP during pyruvate carboxylation?

  • Acetyl-CoA
  • Pyruvate
  • Phosphoenolpyruvate (correct)
  • Oxaloacetate
  • What is the name of the enzyme responsible for oxaloacetate decarboxylation during pyruvate carboxylation?

    <p>Oxaloacetate decarboxylase</p> Signup and view all the answers

    What is the name of the molecule that is produced by pyruvate carboxylation?

    <p>Oxaloacetate</p> Signup and view all the answers

    What is the name of the molecule that is required for C2 phosphorylation during pyruvate carboxylation?

    <p>GTP</p> Signup and view all the answers

    What is the name of the enzyme responsible for catalyzing the reaction of C2 phosphorylation during pyruvate carboxylation?

    <p>Phosphoenolpyruvate carboxykinase</p> Signup and view all the answers

    What is the name of the molecule that is produced by oxaloacetate decarboxylation during pyruvate carboxylation?

    <p>Phosphoenolpyruvate</p> Signup and view all the answers

    What is the name of the molecule that is produced by C2 phosphorylation during pyruvate carboxylation?

    <p>Phosphoenolpyruvate</p> Signup and view all the answers

    What is the name of the molecule that is required for pyruvate carboxylation?

    <p>ATP</p> Signup and view all the answers

    What is the location of pyruvate carboxylase responsible for pyruvate carboxylation?

    <p>In the mitochondrial matrix</p> Signup and view all the answers

    What is the source of energy required for pyruvate carboxylation?

    <p>Hydrolysis of ATP</p> Signup and view all the answers

    What is the product of C2 phosphorylation by GTP during pyruvate carboxylation?

    <p>Phosphoenolpyruvate</p> Signup and view all the answers

    What is the name of the enzyme responsible for oxaloacetate decarboxylation during pyruvate carboxylation?

    <p>Oxaloacetate decarboxylase</p> Signup and view all the answers

    What is the name of the molecule that is produced by pyruvate carboxylation?

    <p>Oxaloacetate</p> Signup and view all the answers

    What is the name of the molecule that is required for C2 phosphorylation during pyruvate carboxylation?

    <p>GTP</p> Signup and view all the answers

    What is the name of the enzyme responsible for catalyzing the reaction of C2 phosphorylation during pyruvate carboxylation?

    <p>Phosphoenolpyruvate carboxykinase</p> Signup and view all the answers

    What is the name of the molecule that is produced by oxaloacetate decarboxylation during pyruvate carboxylation?

    <p>Pyruvate</p> Signup and view all the answers

    What is the name of the molecule that is produced by C2 phosphorylation during pyruvate carboxylation?

    <p>Phosphoenolpyruvate</p> Signup and view all the answers

    What is the name of the molecule that is required for pyruvate carboxylation?

    <p>ATP</p> Signup and view all the answers

    What is the name of the enzyme responsible for pyruvate carboxylation, and where is it present?

    <p>Pyruvate carboxylase, present in the mitochondrial matrix</p> Signup and view all the answers

    What is the source of energy required for pyruvate carboxylation?

    <p>Hydrolysis of ATP</p> Signup and view all the answers

    What is the product of C2 phosphorylation by GTP during pyruvate carboxylation?

    <p>Phosphoenolpyruvate</p> Signup and view all the answers

    What is the name of the enzyme responsible for catalyzing the reaction that converts oxaloacetate to phosphoenolpyruvate?

    <p>PEPCK</p> Signup and view all the answers

    What molecule is released during the decarboxylation of oxaloacetate?

    <p>Carbon dioxide</p> Signup and view all the answers

    What is the role of PEPCK in pyruvate carboxylation?

    <p>To convert oxaloacetate to phosphoenolpyruvate</p> Signup and view all the answers

    Where does the hydrolysis of ATP occur during pyruvate carboxylation?

    <p>In the mitochondrial matrix</p> Signup and view all the answers

    What is the product of pyruvate carboxylation?

    <p>Oxaloacetate</p> Signup and view all the answers

    What is the source of energy for C2 phosphorylation during pyruvate carboxylation?

    <p>Hydrolysis of GTP</p> Signup and view all the answers

    What is the location of pyruvate carboxylase, the enzyme responsible for pyruvate carboxylation?

    <p>Mitochondrial matrix</p> Signup and view all the answers

    Study Notes

    Pyruvate Carboxylation

    • Pyruvate carboxylase, the enzyme responsible for pyruvate carboxylation, is located in the mitochondria.
    • The source of energy required for pyruvate carboxylation is ATP, which is hydrolyzed to ADP and Pi during the process.

    Phosphorylation and Decarboxylation

    • During pyruvate carboxylation, GTP is required for C2 phosphorylation, producing GDP and Pi as byproducts.
    • The product of C2 phosphorylation is phosphoenolpyruvate (PEP).
    • The enzyme responsible for oxaloacetate decarboxylation is phosphoenolpyruvate carboxykinase (PEPCK).
    • CO2 is released during the decarboxylation of oxaloacetate.

    Key Molecules Involved

    • Pyruvate is the molecule that undergoes carboxylation to produce oxaloacetate.
    • GTP is the molecule required for C2 phosphorylation during pyruvate carboxylation.
    • Phosphoenolpyruvate (PEP) is the product of pyruvate carboxylation.

    Enzymes and Their Roles

    • Pyruvate carboxylase catalyzes the reaction of pyruvate carboxylation.
    • Phosphoenolpyruvate carboxykinase (PEPCK) is the enzyme responsible for oxaloacetate decarboxylation.
    • PEPCK also catalyzes the reaction that converts oxaloacetate to phosphoenolpyruvate (PEP).

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    Description

    Test your knowledge on the two crucial steps in gluconeogenesis - pyruvate carboxylation and oxaloacetate decarboxylation. Explore the enzymes involved and the energy sources required to produce phosphoenolpyruvate. Challenge yourself with this quiz and enhance your understanding of the metabolic pathway of gluconeogenesis.

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