Genomics and Applications Quiz
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Questions and Answers

What methods are used in genomic analysis?

  • Immunohistochemistry, flow cytometry, ELISA
  • High-throughput sequencing, microarray hybridization, bioinformatics (correct)
  • Western blotting, chromatography, mass spectrometry
  • Sanger sequencing, PCR amplification, gel electrophoresis
  • What is a characteristic of Next Generation Sequencing (NGS)?

  • Reliance on Sanger sequencing
  • Low computational intensity
  • Massive parallel sequencing method (correct)
  • Limited data output
  • What are some techniques used in Next Generation Sequencing (NGS)?

  • Gel electrophoresis, Western blotting, PCR amplification
  • Mass spectrometry, chromatography, ELISA
  • Flow cytometry, immunohistochemistry, microarray hybridization
  • 454 Sequencing, Illumina/Solexa, ABI SOLiD Sequencing (correct)
  • What is an amplification approach used in NGS?

    <p>Emulsion PCR</p> Signup and view all the answers

    What is the time per Paired End (PED) run for ABI SOLiD sequencing?

    <p>3 Mb per run</p> Signup and view all the answers

    Which sequencing method is subject to error if a single region is not read multiple times?

    <p>454 Sequencing</p> Signup and view all the answers

    What is the characteristic of high-throughput sequencing (NGS) mentioned in the text?

    <p>Huge amount of data (terabytes)</p> Signup and view all the answers

    Which approach is used for amplification in Illumina/Solexa sequencing?

    <p>Bridge amplification</p> Signup and view all the answers

    What is the approximate number of samples per batch in Sanger sequencing?

    <p>384 samples/batch</p> Signup and view all the answers

    Which method is based on massive parallel sequencing?

    <p>454 Sequencing</p> Signup and view all the answers

    What is the primary role of bioinformatics in genomic analysis?

    <p>All of the above</p> Signup and view all the answers

    What is the main challenge associated with high-throughput sequencing (NGS)?

    <p>Handling huge amounts of data</p> Signup and view all the answers

    What is a distinguishing feature of next generation sequencing (NGS) compared to Sanger sequencing?

    <p>Higher sample throughput per batch</p> Signup and view all the answers

    Which method is used in next generation sequencing (NGS) to line up the reads and is subject to error if a single region is not read multiple times?

    <p>Emulsion PCR</p> Signup and view all the answers

    Which sequencing technique is known for its use of polymerase-based sequence-by-synthesis?

    <p>454 Sequencing</p> Signup and view all the answers

    What is the typical size of paired end (PED) separation in next generation sequencing (NGS)?

    <p>3 kb</p> Signup and view all the answers

    Which approach is used in next generation sequencing (NGS) to amplify DNA fragments in a massively parallel manner?

    <p>Emulsion PCR</p> Signup and view all the answers

    What is a key advantage of next generation sequencing (NGS) over traditional Sanger sequencing?

    <p>Higher throughput</p> Signup and view all the answers

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