Genetics: DNA Sequences and Gene Expression
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Questions and Answers

What role does DNA gyrase play in DNA manipulation?

  • It interlocks circular DNA molecules during recombination.
  • It acts as a promoter for gene expression.
  • It introduces negative supercoils into DNA. (correct)
  • It binds to stop codons, terminating translation.
  • Which describes the Pribnow box?

  • Regulatory sequence that binds transcription factors
  • Region of bacterial promoter recognized by RNA polymerase (correct)
  • Sequence of nucleotides that can be translated to give a protein
  • Region of mRNA between the 5' end and the translation start site
  • What is an open reading frame (ORF)?

  • Sequence of bases that can be translated to give a protein (correct)
  • A regulatory sequence inside the promoter region
  • Region of mRNA between the 5' end and the translation start site
  • Original RNA molecule obtained by transcription from a DNA template
  • What happens at the termination stage of translation?

    <p>The polypeptide remains in the P site.</p> Signup and view all the answers

    What is the primary function of housekeeping genes?

    <p>They are needed for essential life functions.</p> Signup and view all the answers

    Which statement describes an enhancer?

    <p>It is a regulatory sequence that binds transcription factors.</p> Signup and view all the answers

    How can catenanes be described?

    <p>Circular DNA molecules that become interlocked during transcription or recombination</p> Signup and view all the answers

    What is a characteristic of a constitutive gene?

    <p>It has promoter sequences very close to consensus.</p> Signup and view all the answers

    Study Notes

    DNA Sequences and Gene Expression

    • Genetic code is used to convert the base sequence in nucleic acids, read in groups of three, into the sequence of a tRNA.
    • Termination occurs when no tRNA exists to bind to a stop codon, causing the tRNA holding the polypeptide to remain in the P site.
    • DNA gyrase is an enzyme that introduces negative supercoils into DNA, and is a member of the type II topoisomerase family.
    • Supercoiling refers to the higher-level coiling of DNA that is already a complementary strand.
    • Catenanes are circular molecules of DNA that can become interlocked during transcription or recombination.

    Gene Structure and Regulation

    • An open reading frame is a sequence of bases (either in DNA or RNA) that can be translated to give a protein.
    • Constitutive genes often have both their -10 and -35 region promoter sequences very close to consensus, which are repressed under rare conditions.
    • Enhancers are regulatory sequences inside, and often far away from, the promoter region that bind transcription factors.
    • The 5'-UTR region of an mRNA is located between the 5' end and the translation start site.
    • Primary transcript is the original RNA molecule obtained by transcription from a DNA template, before any processing or modification has occurred.

    Gene Expression and Regulation

    • Housekeeping genes are genes that are switched on some of the time because they are needed for essential life functions.
    • Pribnow box is a region of bacterial promoter 10 bases back from the start of transcription that is recognized by RNA polymerase.

    True or False

    • A: (F) - There is only one possible model for ordering cell-cycle events, the dependent pathway.

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    Description

    This quiz covers the genetic code, termination of gene expression, DNA gyrase, and supercoiling. Learn about the processes involved in converting DNA sequences into polypeptides

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